Conservation Genetics

, Volume 9, Issue 5, pp 1357–1361

Development of 37 microsatellite loci for the great gray owl (Strix nebulosa) and other Strix spp. owls

  • Joshua M. Hull
  • John J. Keane
  • Lisa A. Tell
  • Holly B. Ernest
Technical Note


We developed 37 great gray owl (Strix nebulosa) microsatellite primers from CA and TAGA enriched genomic libraries. Primers were tested in 15 great gray owls from California, USA and Alberta, Canada as well as two other Strix species, spotted owl (S. occidentalis) and barred owl (S. varia). These markers will have broad application in investigations of Strix population structure and genetic diversity.


Microsatellite Great gray owl Owl Strix nebulosa Strigidae 


  1. Bull EL, Duncan JR (1997) Great gray owl (Strix nebulosa). In Poole A, Gill F (eds) The birds of North America, No. 41. The Academy of Natural Sciences, Philadelphia, and The American Ornithologists’ Union, WashingtonGoogle Scholar
  2. Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50Google Scholar
  3. Jones KC, Levine KF, Banks JD (2002) Characterization of 11 polymorphic tetranucleotide microsatellites for forensic applications in California elk (Cervus elaphus canadensis). Mol Ecol Notes 2(4):425–427CrossRefGoogle Scholar
  4. Hsu YC, Li SH, Lin YS, Severinghaus LL (2006) Microsatellite loci from Lanyu Scops Owl (Otus elegans botelensis) and their cross amplification in four species of Strigidae. Conserv Genet 7:161–165CrossRefGoogle Scholar
  5. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  6. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
  7. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar
  8. Toonen RJ, Hughes S (2001) Increased throughput for fragment analysis on an ABI Prism® 377 automated sequencer using a membrane comb and STRand software. Biotechniques 31(6):1320–1324PubMedGoogle Scholar
  9. Wilberg MJ, Dreher BP (2004) GENECAP: a program for analysis of multilocus genotype data for non-invasive sampling and capture-recapture population estimation. Mol Ecol Notes 4(4):783CrossRefGoogle Scholar
  10. Winter J (1986) Status, distribution, and ecology of the Great Gray Owl in California. M.A. Thesis, San Francisco State UniversityGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Joshua M. Hull
    • 1
  • John J. Keane
    • 2
  • Lisa A. Tell
    • 3
  • Holly B. Ernest
    • 1
    • 4
  1. 1.Wildlife and Ecology Unit, Veterinary Genetics LaboratoryUniversity of CaliforniaDavisUSA
  2. 2.Sierra Nevada Research Center, Pacific Southwest Research StationUnited States Department of Agriculture, Forest ServiceDavisUSA
  3. 3.Department of Medicine and Epidemiology, School of Veterinary MedicineUniversity of CaliforniaDavisUSA
  4. 4.Department of Population Health and Reproduction, School of Veterinary MedicineUniversity of CaliforniaDavisUSA

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