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Conservation Genetics

, Volume 9, Issue 6, pp 1467–1477 | Cite as

Phylogeography and population structure of the endangered Tehuantepec jackrabbit Lepus flavigularis: implications for conservation

  • Yessica Rico
  • Consuelo Lorenzo
  • Francisco X. González-Cózatl
  • Eduardo Espinoza
Research Article

Abstract

The Tehuantepec jackrabbit (Lepus flavigularis) is an endangered species restricted to a small area in the Isthmus of Tehuantepec, Oaxaca, Mexico. To evaluate its phylogeographic structure, population genetics, and demographic history we sequenced the mitochondrial Control Region hypervariable domain (CR-1) for 42 individuals representing the entire species range. Phylogenetic patterns indicated that this species is subdivided into two highly divergent clades, with an average nucleotide genetic distance of 3.7% (TrN) between them. Clades A and B are geographically distributed in non-overlapping areas to the west and to the east of the Isthmus of Tehuantepec, respectively. Genetic diversity indices showed reduced genetic variability in L. flavigularis when compared to other species of Lepus within main clades and within populations. This low genetic diversity coupled with the restricted distribution to very small areas of occurrence and limited gene flow suggest that genetic drift has played an important role in the evolution of this species. Historical demographic analysis also pointed out that these two clades underwent a recent population expansion that started about 9,000 years ago for clade A and 3,200 years ago for clade B during the Holocene. Consequently, from the conservation perspective our results suggest that populations included in clades A and B should be regarded as distinct evolutionary lineages.

Keywords

Lepus flavigularis Phylogeography Genetic diversity Population structure Control region Conservation 

Notes

Acknowledgments

We are grateful to Roberto Gutiérrez, Roberto Gutiérrez Jr., Leyberto Gutiérrez, Juan Antonio and family, Jorge Bolaños and the Municipality of Santa María del Mar for their valuable help during field work. We thank the families Gutiérrez-López and Gutiérrez-Vázquez for housing our crew. We are grateful to Fernando Cervantes, who provided tissue samples of L. callotis. Also, we thank Arturo Carrillo for his help on map editing. Constructive comments on the manuscript were provided by Duke S. Rogers and anonymous reviewers. This project was partially funded by the Consejo Nacional de Ciencia y Tecnología and Gobierno del Estado de Chiapas (project CHIS-2005-CO3-001) and El Colegio de la Frontera Sur (PATM project 11022). Instituto Nacional de Ecología, SEMARNAT, issued a special collecting permit (SGPA/DGVS/01468/06) that allowed us to catch specimens of L. flavigularis, take ear clips, and then release them.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Yessica Rico
    • 1
  • Consuelo Lorenzo
    • 1
  • Francisco X. González-Cózatl
    • 2
  • Eduardo Espinoza
    • 1
  1. 1.El Colegio de la Frontera SurSan Cristóbal de Las CasasMexico
  2. 2.Centro de Educación Ambiental e Investigación Sierra de HuautlaUniversidad Autónoma del Estado de MorelosCuernavacaMexico

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