Conservation Genetics

, Volume 9, Issue 5, pp 1333–1335 | Cite as

Isolation and characterization of 11 polymorphic microsatellite loci from Fatsia polycarpa (Araliaceae), an element of evergreen forests in Taiwan

  • Chi-Chun Huang
  • Kuo-Hsiang Hung
  • Tsai-Wen Hsu
  • Kuo-Hsiung Wang
  • Chi-Yung Lin
  • Tzen-Yuh Chiang
Technical Note
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Abstract

Fatsia polycarpa Hayata (Araliaceae) is an element of evergreen forests at middle elevations in Taiwan, also representing the southern limitation in distribution of the genus. In this study, we described the development of 11 microsatellite loci from F. polycarpa in Taiwan for genetic studies. These new markers were tested in 20 individuals of 5 populations of the woody species. The number of alleles ranged from 9 to 18. The expected (H E ) and observed (H O ) heterozygosities were 0.80–0.95 and 0.10–0.75, respectively. Six of the 11 microsatellite loci are significantly deviated from Hardy–Weinberg expectations likely due to the population structure within samples. No linkage disequilibrium were observed from pairwise comparisons of loci.

Keywords

Fatsia polycarpa Microsatellite PIMA RAPD-PCR enrichment Taiwan 
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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Chi-Chun Huang
    • 1
  • Kuo-Hsiang Hung
    • 1
  • Tsai-Wen Hsu
    • 2
  • Kuo-Hsiung Wang
    • 1
  • Chi-Yung Lin
    • 1
  • Tzen-Yuh Chiang
    • 1
  1. 1.Department of Life SciencesCheng Kung UniversityTainanTaiwan
  2. 2.Taiwan Endemic Species Research InstituteNantouTaiwan

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