Development of ten microsatellite markers for Quercus mongolica var. crispula by database mining
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Simple sequence repeats (SSRs) in the NCBI dbEST database were surveyed to identify potential SSR markers for Quercus mongolica. In total, 2,691 gene sequences, mainly from expressed sequence tags (ESTs) for Q. robur and Q. petraea had been registered. Twenty-two PCR primers were designed for SSRs in these sequences and screened for polymorphisms in 16 Q. mongolica trees. Ten loci were easily genotyped and showed polymorphism, with numbers of alleles and expected heterozygosity ranging from 3 to 15 and 0.28 to 0.94, respectively. These EST-SSR markers should be useful for studying the genetic diversity of Quercus species.
KeywordsExpressed sequence tag Fagaceae Quercus petraea Quercus robur SSR
The authors are grateful to M. Kanno, A. Kanazashi, H. Yoshimaru, K. Ishida, Y. Koyama and K. Koono for sample collection and to Y. Taguchi for laboratory work. This research was supported by a grant for Research on Genetic Guideline for Restoration Programs using Genetic Diversity Information from the Ministry of Environment, Japan.
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