Conservation Genetics

, Volume 9, Issue 4, pp 1051–1054 | Cite as

Development of EST-microsatellites from the cycad Cycas rumphii, and their use in the recently endangered Cycas micronesica

  • Angélica Cibrián-Jaramillo
  • Thomas E. Marler
  • Rob DeSalle
  • Eric D. Brenner
Technical Note


We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform conservation efforts and disentangle the biogeographic history of the genus.


Cycad Cycas micronesica EST-STRs Invasives 



We are grateful to D. Little and D. Stevenson for collecting all but Cycas micronesica samples, and the Florida Montgomery Botanical Center for kindly providing them. We also thank Venkatesh Alapati and Helen Ma for their contribution in the processing of the samples. This research was supported by the U.S. Department of Agriculture CSREES Award 2006-34135-17141.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Angélica Cibrián-Jaramillo
    • 1
    • 2
  • Thomas E. Marler
    • 3
  • Rob DeSalle
    • 2
  • Eric D. Brenner
    • 1
  1. 1.GenomicsThe New York Botanical Garden BronxUSA
  2. 2.Sackler Institute for Comparative GenomicsAmerican Museum of Natural HistoryNew YorkUSA
  3. 3.Western Pacific Tropical Research Center, College of Natural and Applied Sciences University of GuamGuamUSA

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