Development of EST-microsatellites from the cycad Cycas rumphii, and their use in the recently endangered Cycas micronesica
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We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform conservation efforts and disentangle the biogeographic history of the genus.
KeywordsCycad Cycas micronesica EST-STRs Invasives
We are grateful to D. Little and D. Stevenson for collecting all but Cycas micronesica samples, and the Florida Montgomery Botanical Center for kindly providing them. We also thank Venkatesh Alapati and Helen Ma for their contribution in the processing of the samples. This research was supported by the U.S. Department of Agriculture CSREES Award 2006-34135-17141.
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