Conservation Genetics

, Volume 9, Issue 4, pp 973–979

Development of new microsatellite primers for green and white sturgeon

  • Karrigan Börk
  • A. Drauch
  • J. A. Israel
  • J. Pedroia
  • J. Rodzen
  • B. May
Technical Note

DOI: 10.1007/s10592-007-9417-9

Cite this article as:
Börk, K., Drauch, A., Israel, J.A. et al. Conserv Genet (2008) 9: 973. doi:10.1007/s10592-007-9417-9

Abstract

Sixty-eight primer sets for microsatellite loci were developed from microsatellite motif enriched genomic libraries of pooled DNA from the polyploid green and white sturgeon (Acipenser medirostris and A. transmontanus). Four individuals from each species were screened for polymorphism at these loci. Forty-eight loci amplified in both species, and some exhibited species-specific amplification for white or green sturgeon (8 and 12 loci, respectively). The number of alleles per locus ranged from one to 12. At least 68% of the green and 65% of the white sturgeon loci we developed are polysomic.

Keywords

Microsatellite Sturgeon Acipenser Primer 

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Karrigan Börk
    • 1
    • 2
  • A. Drauch
    • 1
  • J. A. Israel
    • 1
  • J. Pedroia
    • 1
  • J. Rodzen
    • 3
  • B. May
    • 1
  1. 1.Genomic Variation Lab, Animal Science DepartmentUC DavisDavisUSA
  2. 2.Redwood CityUSA
  3. 3.Wildlife Forensic Laboratory, California Department of Fish & GameRancho CordovaUSA

Personalised recommendations