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Conservation Genetics

, Volume 9, Issue 3, pp 767–769 | Cite as

Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)

  • Qian Zhang
  • Yan-Zhuo Yang
  • Gui-Li Wu
  • Dong-Yuan Zhang
  • Jian-Quan LiuEmail author
Technical Note

Abstract

Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined. In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species. In addition, all markers have been crossly checked in the other four congeneric species.

Keywords

Juniperus przewalskii Microsatellite markers Genetic diversity 

Notes

Acknowledgements

This research was supported by Natural Science Foundation of China (30430560) and International Foundation for Science (IFS), Stockholm, Sweden AD/17475) and the Education Ministry.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Qian Zhang
    • 1
  • Yan-Zhuo Yang
    • 1
  • Gui-Li Wu
    • 1
  • Dong-Yuan Zhang
    • 1
  • Jian-Quan Liu
    • 1
    • 2
    Email author
  1. 1.MOE Key Laboratory of Arid and Grassland Ecology, School of Life SciencesLanzhou UniversityLanzhouChina
  2. 2.Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of BiologyChinese Academy of SciencesXiningChina

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