Conservation Genetics

, Volume 9, Issue 3, pp 767–769 | Cite as

Isolation and characterization of microsatellite DNA primers in Juniperus przewalskii Kom (Cupressaceae)

  • Qian Zhang
  • Yan-Zhuo Yang
  • Gui-Li Wu
  • Dong-Yuan Zhang
  • Jian-Quan LiuEmail author
Technical Note


Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined. In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species. In addition, all markers have been crossly checked in the other four congeneric species.


Juniperus przewalskii Microsatellite markers Genetic diversity 



This research was supported by Natural Science Foundation of China (30430560) and International Foundation for Science (IFS), Stockholm, Sweden AD/17475) and the Education Ministry.


  1. Clarke KR, Gorley RN (2001) PRIMER v5: User Manual/Tutorial. PRIMER-E Ltd., Plymouth, pp 91Google Scholar
  2. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem Bullet 19:11–15Google Scholar
  3. Hauswaldt JS, Glenn TC (2003) Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin). Mol Ecol Notes 3:174–176CrossRefGoogle Scholar
  4. Korfanta NM, Schable NA, Glenn TC (2002) Isolation and characterization of microsatellite DNA primers in burrowing owl (Athene cunicularia). Mol Ecol Notes 2:584–585CrossRefGoogle Scholar
  5. Michalczyk IM, Sebastiani F, Buonamici A, Cremer E, Mengel C, Ziegenhagen B, Vendramin GG (2006) Characterization of highly polymorphic nuclear microsatellite loci in Juniperus communis L. Mol Ecol Notes 6:346–348CrossRefGoogle Scholar
  6. Primmer CR, Moller AP, Ellengren H (1996) A wide-range survey of cross species microsatellite amplifications in birds. Mol Ecol 5:365–378PubMedCrossRefGoogle Scholar
  7. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  8. Zhang Q, Chiang TY, Miehe G, Liu JQ, Abbott R (2005) Phylogeography of the Qinghai-Tibetan Plateau endemic Juniperus przewalskii (Cupressaceae) inferred from chloroplast DNA sequence variation. Mol Ecol 14:3513–3524PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Qian Zhang
    • 1
  • Yan-Zhuo Yang
    • 1
  • Gui-Li Wu
    • 1
  • Dong-Yuan Zhang
    • 1
  • Jian-Quan Liu
    • 1
    • 2
    Email author
  1. 1.MOE Key Laboratory of Arid and Grassland Ecology, School of Life SciencesLanzhou UniversityLanzhouChina
  2. 2.Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of BiologyChinese Academy of SciencesXiningChina

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