Development and characterization of microsatellite markers for an endangered shrub, Ammopiptanthus mongolicus (Leguminosae) and cross-species amplification in Ammopiptanthus nanus
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We developed 11 polymorphic microsatellite markers for an endangered shrub Ammopiptanthus mongolicus using the Fast Isolation by AFLP of Sequences Containing repeats protocol. Polymorphism of each locus was assessed in 24 individuals from five natural populations of A. mongolicus. The average allele number of these markers was 4.2, ranging from 2 to 7. The observed (H O) and expected (H E) heterozygosities were 0.044–0.956 and 0.044–0.827, respectively. Further assessment in the congeneric species A. nanus revealed that nine of eleven loci were successfully amplified, but only two loci showed polymorphism. These markers provide powerful tools for the conservation genetics studies of A. mongolicus.
KeywordsAmmopiptanthus mongolicus Endangered species FIASCO Genetic diversity Microsatellite markers
We are grateful to YF Liu, YQ Xu for their technical assistance. This work was financially supported by the National Natural Foundation of China (Grant No. 30370282) and the Key Project of the Chinese Academy of Sciences (CAS) (KSCX2-SW-104).
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