Conservation Genetics

, Volume 9, Issue 1, pp 225–228

PCR-CTPP: a rapid and reliable genotyping technique based on ZFX/ZFY alleles for sex identification of tiger (Panthera tigris) and four other endangered felids

  • Kun Wei
  • Zhihe Zhang
  • Wenping Zhang
  • Xiao Xu
  • Xu Liang
  • Guangxin He
  • Fujun Shen
  • Liang Zhang
  • Rong Hou
  • Bisong Yue
Short Communication

Abstract

An inexpensive, time-saving and reliable method, polymerase chain reaction with confronting two-pair primers (PCR-CTPP), was developed for sex identification in tiger (Panthera tigris) based on zinc finger alleles (ZFX/ZFY). A site of “C/G” transversion representing fixed differences that discriminated between ZFX and ZFY exons among felids was identified for primers designing. This primer set was successfully tested on samples including blood, shed hairs, dried skin, and stool which contained potential contamination caused by prey DNA. Cross species tests shown that this primer set was also useful for sex identification in four other endangered felids.

Keywords

Felids PCR-CTPP Sex identification Tiger ZFX ZFY 

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Kun Wei
    • 1
  • Zhihe Zhang
    • 2
  • Wenping Zhang
    • 1
    • 3
  • Xiao Xu
    • 1
  • Xu Liang
    • 1
  • Guangxin He
    • 2
  • Fujun Shen
    • 1
    • 2
  • Liang Zhang
    • 2
  • Rong Hou
    • 2
  • Bisong Yue
    • 1
  1. 1.Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life SciencesSichuan UniversityChengduP.R. China
  2. 2.Key Laboratory of Endangered Wildlife Breeding and Conservation Genetics of Sichuan ProvinceChengdu Research Base of Giant Panda BreedingChengduP.R. China
  3. 3.College of ScienceHonghe UniversityMengziP.R. China

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