Conservation Genetics

, Volume 8, Issue 5, pp 1219–1224

The impact of time and field conditions on brown bear (Ursus arctos) faecal DNA amplification

  • Melanie A. Murphy
  • Katherine C. Kendall
  • Andrew Robinson
  • Lisette P. Waits
Short Communications

Abstract

To establish longevity of faecal DNA samples under varying summer field conditions, we collected 53 faeces from captive brown bears (Ursus arctos) on a restricted vegetation diet. Each faeces was divided, and one half was placed on a warm, dry field site while the other half was placed on a cool, wet field site on Moscow Mountain, Idaho, USA. Temperature, relative humidity, and dew point data were collected on each site, and faeces were sampled for DNA extraction at <1, 3, 6, 14, 30, 45, and 60 days. Faecal DNA sample viability was assessed by attempting PCR amplification of a mitochondrial DNA (mtDNA) locus (∼150 bp) and a nuclear DNA (nDNA) microsatellite locus (180–200 bp). Time in the field, temperature, and dew point impacted mtDNA and nDNA amplification success with the greatest drop in success rates occurring between 1 and 3 days. In addition, genotyping errors significantly increased over time at both field sites. Based on these results, we recommend collecting samples at frequent transect intervals and focusing sampling efforts during drier portions of the year when possible.

Keywords

DNA preservation Faecal DNA Noninvasive genetic sampling Ursus arctos 

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Melanie A. Murphy
    • 1
    • 2
  • Katherine C. Kendall
    • 3
  • Andrew Robinson
    • 4
    • 5
  • Lisette P. Waits
    • 1
  1. 1.Department of Fish and Wildlife ResourcesUniversity of IdahoMoscowUSA
  2. 2.School of Biological SciencesWashington State UniversityPullmanUSA
  3. 3.USGS Northern Rocky Mountain Science CenterGlacier Field StationWest GlacierUSA
  4. 4.Department of Forest ResourcesUniversity of IdahoMoscowUSA
  5. 5.Department of Mathematics and StatisticsUniversity of MelbourneVICAustralia

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