Conservation Genetics

, Volume 8, Issue 5, pp 1263–1266

Isolation and characterization of microsatellites in Primula glaucescens Moretti and their cross-species amplification in P. spectabilis Tratt.

  • Davide Perini
  • Marilena Meloni
  • Kirsten Wolff
  • Giorgio Binelli
Technical note

Abstract

The hexaploid herbaceous species Primula glaucescens Moretti and Primula spectabilis Tratt. (2= 6= 66) are two endemics of the Italian Pre-Alps protected by national and international laws. In order to plan conservation strategies for natural populations and to study the influence of the latest glaciation on them we developed a set of microsatellites markers for the endangered Primula species: ten primer pairs allowed analysis of polymorphic disomic loci in P. glaucescens samples and seven of them also amplified polymorphic disomic markers in P. spectabilis.

Keywords

Conservation and repopulation Microsatellite enriched library Hexaploid alpine plant species Primula glaucescens Moretti Primula spectabilis Tratt. 

References

  1. Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (1996–2004) GENETIX 4.05, logiciel sous Windows TM pour la génétique des populations. Laboratoire Génome, Populations, Interactions, CNRS UMR 5000. Université de Montpellier II, Montpellier, FranceGoogle Scholar
  2. Conti F, Manzi A, Pedrotti F (1997) Liste Rosse Regionali delle Piante d’Italia. WWF Italia - Società Botanica Italiana, Camerino Google Scholar
  3. Hamilton MB, Pincus EL, Di Fiore A, Flescher RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellite. Biotechnique 27:500–507Google Scholar
  4. Kadereit JW, Griebeler EM, Comes HP (2004). Quaternary diversification in European alpine plants: pattern and process. Phil Trans R. Soc Lond. B 359:265–274Google Scholar
  5. Pawlowski B (1970) Remarques sur l’endémisme dans la flore des Alpes et des Carpates. Vegetatio 21:181–243CrossRefGoogle Scholar
  6. Perini D (2005) Conservation genetics of alpine plant species: development of genetic markers (SSRs) and their application to a case study, Primula glaucescens Moretti. PhD dissertation, Università degli Studi dell’InsubriaGoogle Scholar
  7. Ravazzi C (1999) Distribuzione ed ecologia di due primule endemiche delle prealpi calcaree meridionali, Primula glaucescens e Primula spectabilis, e considerazioni sulla loro corogenesi. Arch Geobot 3:125–148Google Scholar
  8. Richards J (2003) Primula. BT Batsford Ltd, LondonGoogle Scholar
  9. Rozen S, Skaletsky HJ (2000) PRIMER 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, New Jersey, pp 365–386. Source code available at http://fokker.wi.mit.edu/primer3/Google Scholar
  10. Schönswetter P, Stehlik I, Holderregger R, Tribsh A (2005) Molecular evidence for glacial refugia of mountain plants in the European Alps. Mol Ecol 14:3547–3555PubMedCrossRefGoogle Scholar
  11. Stehlik I (2000) Nunataks and peripheral refugia for alpine plants during Quaternary glaciation in the middle part of the Alps. Bot Helv 110:25–30Google Scholar
  12. Stehlik I (2003) Resistance or emigration? Response of alpine plants to the ice ages. Taxon 52:499–510CrossRefGoogle Scholar
  13. Stehlik I, Holderegger R, Schneller JJ, Abbott RJ, Bachmann K (2000) Molecular biogeography and population genetics of alpine plant species. Bull Geobot Inst ETH 66:47–59Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Davide Perini
    • 1
    • 2
    • 3
  • Marilena Meloni
    • 1
  • Kirsten Wolff
    • 2
  • Giorgio Binelli
    • 1
  1. 1.Dipartimento di Biotecnologie e Scienze Molecolari (DBSM)Università degli Studi dell’InsubriaVareseItaly
  2. 2.School of Biology & Psychology, Division of BiologyUniversity of Newcastle upon TyneNewcastle upon TyneUK
  3. 3.Dipartimento di Scienze Biomolecolari e Biotecnologie (DSBB)Università degli Studi di MilanoMilanoItaly

Personalised recommendations