Conservation Genetics

, Volume 8, Issue 4, pp 1013–1016 | Cite as

Isolation and characterisation of polymorphic microsatellite loci in the vulnerable spectacled flying fox, Pteropus conspicillatus

Technical Note

Abstract

The spectacled flying fox, Pteropus conspicillatus, is listed as vulnerable in Australia and is under threat from numerous impacts. Primers to amplify eight co-dominant microsatellite loci were designed for Pteropus conspicillatus, based on an enriched genomic library. Four loci were monomorphic in this species while the remaining four loci were highly polymorphic with 16–23 alleles. Two of the four monomorphic loci were found to be polymorphic in Pteropus alecto, a closely related congener. All but one of the six polymorphic loci were in Hardy Weinberg equilibrium. Additionally, six microsatellite loci isolated for Pteropus rodricensis were tested against individuals of P. conspicillatus with all loci amplifying reliably. These loci will be used to investigate population genetic structure in the vulnerable spectacled flying fox.

Keywords

Microsatellites Flying fox Pteropodidae 

Notes

Acknowledgements

This work was funded by the Rainforest CRC, an ARC Linkage Grant, and Queensland Fruit and Vegetable Growers Association.

References

  1. Cobb BD, Clarkson JM (1994) A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods. Nucleic Acids Res 22(18):3801–3805PubMedCrossRefGoogle Scholar
  2. Cox PA, Elmqvist T, Pierson ED, Rainey WE (1992) Flying foxes as pollinators and seed dispersers in Pacific Island ecosystems. In: Wilson DE and Graham GL (eds) Pacific island flying-foxes: proceedings of an international conservation conference, Hawaii, U.S. Fish and Wildlife Service, pp 176Google Scholar
  3. Garnett S, Whybird O, Spencer H (1999) The conservation status of the Spectacled Flying fox Pteropus conspicillatus in Australia. Aust Zool 31:38–54Google Scholar
  4. Glenn TC, Cary T, Dust M, Hauswaldt S, Prince K, Ramsdell C, Shute I (2000) Microsatellite isolation 2000. In: Biology 656 summer class manual, University of South Caroline, Columbia, pp 1–28Google Scholar
  5. Hamilton MB, Pincus EL, Di Fiore A, Fleisher, RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. BioTechniques 27:500–507PubMedGoogle Scholar
  6. McConkey KR, Drake DR (2006) Flying foxes cease to function as seed dispersers long before they become rare. Ecology 87:271–276PubMedCrossRefGoogle Scholar
  7. Myers N, Mittermeier RA, Mittermeier CG, da Fonseca GAB, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858PubMedCrossRefGoogle Scholar
  8. Rainey WE, Pierson ED, Elmqvist T, Cox PA (1995) The role of flying foxes (Pteropodidae) in oceanic island ecosystems of the Pacific. Zool Symp 67:47–62Google Scholar
  9. Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Heredity 86:248–249Google Scholar
  10. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa NJ, pp 365–386 Source code available at http://fokker.wi.mit.edu/primer3/Google Scholar
  11. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, New YorkGoogle Scholar
  12. Shilton LA, Altringham JD, Compton SG, Whittaker RJ (1999) Old world fruit bats can be long-distance seed dispersers through extended retention of viable seeds in the gut. Proc R Soc Lond B 266:219–223CrossRefGoogle Scholar
  13. Sokal RR, Rohlf FJ (1995) Biometry: the principles and practice of statistics in biological research. WH Freeman and Company, New YorkGoogle Scholar
  14. Tidemann C, Kelson S, Jamieson G (1997) Flying fox damage to orchard fruit in Australia – incidence, extent and economic impact. Aust Biol 10:177–184Google Scholar
  15. Westcott DA, Dennis AJ, Bradford MG, Margules CR (2001) The Spectacled Flying fox, Pteropus conspicillatus, in the context of the world heritage values of the Wet Tropics World Heritage Area. Environment Australia, Atherton, pp 75Google Scholar
  16. Wiles GJ, Fujita MS (1992) Food plants and economic importance of flying-foxes on Pacific Islands. In: Wilson DE, Graham GL (eds) Pacific island flying-foxes: proceedings of an international conservation conference, Hawaii, U.S. Fish and Wildlife Service, pp 176Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Samantha Fox
    • 1
    • 2
  • Michelle Waycott
    • 1
  • Glenn Dunshea
    • 1
  1. 1.School of Marine and Tropical BiologyJames Cook UniversityTownsvilleAustralia
  2. 2.School of Tropical Environment Studies and GeographyJames Cook UniversityTownsvilleAustralia

Personalised recommendations