Isolation and characterisation of polymorphic microsatellite loci in the vulnerable spectacled flying fox, Pteropus conspicillatus
The spectacled flying fox, Pteropus conspicillatus, is listed as vulnerable in Australia and is under threat from numerous impacts. Primers to amplify eight co-dominant microsatellite loci were designed for Pteropus conspicillatus, based on an enriched genomic library. Four loci were monomorphic in this species while the remaining four loci were highly polymorphic with 16–23 alleles. Two of the four monomorphic loci were found to be polymorphic in Pteropus alecto, a closely related congener. All but one of the six polymorphic loci were in Hardy Weinberg equilibrium. Additionally, six microsatellite loci isolated for Pteropus rodricensis were tested against individuals of P. conspicillatus with all loci amplifying reliably. These loci will be used to investigate population genetic structure in the vulnerable spectacled flying fox.
KeywordsMicrosatellites Flying fox Pteropodidae
This work was funded by the Rainforest CRC, an ARC Linkage Grant, and Queensland Fruit and Vegetable Growers Association.
- Cox PA, Elmqvist T, Pierson ED, Rainey WE (1992) Flying foxes as pollinators and seed dispersers in Pacific Island ecosystems. In: Wilson DE and Graham GL (eds) Pacific island flying-foxes: proceedings of an international conservation conference, Hawaii, U.S. Fish and Wildlife Service, pp 176Google Scholar
- Garnett S, Whybird O, Spencer H (1999) The conservation status of the Spectacled Flying fox Pteropus conspicillatus in Australia. Aust Zool 31:38–54Google Scholar
- Glenn TC, Cary T, Dust M, Hauswaldt S, Prince K, Ramsdell C, Shute I (2000) Microsatellite isolation 2000. In: Biology 656 summer class manual, University of South Caroline, Columbia, pp 1–28Google Scholar
- Rainey WE, Pierson ED, Elmqvist T, Cox PA (1995) The role of flying foxes (Pteropodidae) in oceanic island ecosystems of the Pacific. Zool Symp 67:47–62Google Scholar
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Heredity 86:248–249Google Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa NJ, pp 365–386 Source code available at http://fokker.wi.mit.edu/primer3/Google Scholar
- Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, New YorkGoogle Scholar
- Sokal RR, Rohlf FJ (1995) Biometry: the principles and practice of statistics in biological research. WH Freeman and Company, New YorkGoogle Scholar
- Tidemann C, Kelson S, Jamieson G (1997) Flying fox damage to orchard fruit in Australia – incidence, extent and economic impact. Aust Biol 10:177–184Google Scholar
- Westcott DA, Dennis AJ, Bradford MG, Margules CR (2001) The Spectacled Flying fox, Pteropus conspicillatus, in the context of the world heritage values of the Wet Tropics World Heritage Area. Environment Australia, Atherton, pp 75Google Scholar
- Wiles GJ, Fujita MS (1992) Food plants and economic importance of flying-foxes on Pacific Islands. In: Wilson DE, Graham GL (eds) Pacific island flying-foxes: proceedings of an international conservation conference, Hawaii, U.S. Fish and Wildlife Service, pp 176Google Scholar