Buccal swabs allow efficient and reliable microsatellite genotyping in amphibians
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Buccal swabs have recently been used as a minimally invasive sampling method in genetic studies of wild populations, including amphibian species. Yet it is not known to date what is the level of reliability for microsatellite genotypes obtained using such samples. Allelic dropout and false alleles may affect the genotyping derived from buccal samples. Here we quantified the success of microsatellite amplification and the rates of genotyping errors using buccal swabs in two amphibian species, the Alpine newt Triturus alpestris and the Green tree frog Hyla arborea, and we estimated two important parameters for downstream analyses, namely the number of repetitions required to achieve typing reliability and the probability of identity among genotypes. Amplification success was high, and only one locus tested required two to three repetitions to achieve reliable genotypes, showing that buccal swabbing is a very efficient approach allowing good quality DNA retrieval. This sampling method which allows avoiding the controversial toe-clipping will likely prove very useful in the context of amphibian conservation.
KeywordsNon-destructive sampling Amplification success Genotyping errors Probability of identity Hyla arborea Triturus alpestris
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We are grateful to Nathaniel Valière who kindly answered our questions about the software Gemini, to Jérôme Pellet who provided H. arborea tissue samples, and to an anonymous referee who made useful suggestions. This work was supported by Swiss National Fund grant No. 3100A0-108100 to Nicolas Perrin.
- Miller HC (2006) Cloacal and buccal swabs are a reliable source of DNA for microsatellite genotyping of reptiles. Conserv Genet DOI 10.1007/s10592-006-9120-2Google Scholar
- Pidancier N, Miquel C, Miaud C (2003) Buccal swabs as a non-destructive tissue sampling method for DNA analysis in amphibians. Herpetolo J 13:175–178Google Scholar
- Valière N, Berthier P, Mouchiroud D, Pontier D (2002) GEMINI: software for testing the effects of genotyping errors and multitubes approach for individual identification. Mol Ecol Notes 2:83–86Google Scholar