Conservation Genetics

, Volume 7, Issue 5, pp 799–802 | Cite as

Species and sex identification from faecal samples of sympatric carnivores, Amur leopard and Siberian tiger, in the Russian Far East

  • Taro Sugimoto
  • Junco Nagata
  • Vladimir V. Aramilev
  • Alexander Belozor
  • Seigo Higashi
  • Dale R. McCullough
Technical note

Keywords

Cytb DBY faeces sex identification species identification Zfx 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Eizirik E, Kim JH, Menotti-Raymond M, Crawshaw PG, O’Brien SJ, Johnson WE (2001) Phylogeography, population history and conservation genetics of jaguars (Panthera onca, Mammalia, Felidae). Mol. Ecol. 10: 65–79PubMedCrossRefGoogle Scholar
  2. Goossens B, Chikhi L, Utami SS, Ruiter JD, Bruford MW (2000) A multi-samples, multi-extracts approach for microsatellite analysis of faecal samples in an arboreal ape. Conserv. Genet. 1: 157–162CrossRefGoogle Scholar
  3. Griffiths R, Double MC, Orr K, Dawson RJG (1998) A DNA test to sex most birds. Mol. Ecol. 7: 1071–1075PubMedCrossRefGoogle Scholar
  4. Hellborg L, Ellegren H (2003) Y chromosome conserved anchored tagged sequences (YCATS) for the analysis of mammalian male-specific DNA. Mol. Ecol. 12: 283–291PubMedCrossRefGoogle Scholar
  5. Irwin DM, Kocher TD, Wilson AC (1991) Evolution of the cytochrome b gene of mammals. J. Mol. Evol. 32: 128–144PubMedGoogle Scholar
  6. Kocher TD, Thomas WK, Meyer A et al. (1989) Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers. Proc. Nat. Acad. Sci. USA 86: 6196–6200PubMedCrossRefGoogle Scholar
  7. Kohn MH, Wayne RK (1997) Facts from feces revisited. TREE 12: 223–227Google Scholar
  8. Miquelle DG, Pikunov DG (2003) Status of the Amur tiger and Far Eastern leopards. In: The Russian Far East: A Reference Guide for Conservation and Development (ed.␣Newell JP), pp.␣106–109. Daniel & Daniel, Publishers, Inc., McKinleyville, CAGoogle Scholar
  9. Nagata J, Aramilev VV, Belozor A, Sugimoto T, McCullough DR (2005) Fecal genetic analysis using PCR-RFLP of cytochrome b to identify sympatric carnivores, the tiger Panthera tigris and the leopard Panthera pardus, in far eastern Russia. Conserv. Genet., d.o.i 10.1007/s10592-005-9038-0Google Scholar
  10. Schliebe SL, Evans TJ, Fischbach AS, Cronin MA (1999) Using genetics to verify sex of harvested polar bears: Management implications. Wildl. Soc. Bull. 27: 592–597Google Scholar
  11. Slattery JP, O’Brien SJ (1998) Patterns of Y and X chromosome DNA sequence divergence during the felidae radiation. Genetics 148: 1245–1255Google Scholar
  12. Smith KL, Alberts SC, Bayes MK, Bruford MW, Altmann J, Ober C (2000) Cross-species amplification, non-invasive genotyping, and non-Mendelian inheritance of human STRPs in savannah baboons. Am. J. Primatol. 51: 219–227PubMedCrossRefGoogle Scholar
  13. Taberlet P, Griffin S, Goossens B, Questiau S, Manceau V, Escaravage N, Waits LP, Bouvet J (1996) Reliable genotyping of samples with very low DNA quantities using PCR. Nucl. Acids Res. 24: 3189–3194PubMedCrossRefGoogle Scholar
  14. Taberlet P, Luikart G (1999) Non-invasive genetic sampling and individual identification. Biol. J. Linnean Soc. 68: 41–55CrossRefGoogle Scholar
  15. Taberlet P, Waits LP, Luikart G (1999) Noninvasive genetic sampling: Look before you leap. TREE 14: 323–327PubMedGoogle Scholar
  16. Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10: 506–513PubMedGoogle Scholar
  17. Wehausen JD, Ramey RR, II, Epps CW (2004) Experiments in DNA extraction and PCR amplification from bighorn sheep feces: The importance of DNA extraction method. J. Hered. 95: 503–509PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Taro Sugimoto
    • 1
  • Junco Nagata
    • 2
    • 4
  • Vladimir V. Aramilev
    • 3
  • Alexander Belozor
    • 3
  • Seigo Higashi
    • 1
  • Dale R. McCullough
    • 4
  1. 1.Graduate School of Environmental Earth ScienceHokkaido UniversitySapporoJapan
  2. 2.Forestry and Forest Products Research InstituteTsukubaJapan
  3. 3.Institute of Sustainable Use of Natural ResourcesVladivostokRussia
  4. 4.Department of Environmental Science, Policy and ManagementUniversity of CaliforniaBerkeleyUSA

Personalised recommendations