Conservation Genetics

, Volume 7, Issue 5, pp 631–649 | Cite as

Restoration genetics of the vernal pool endemic Lasthenia conjugens (Asteraceae)

  • Jennifer M. Ramp
  • Sharon K. Collinge
  • Tom A. Ranker


Restoration of habitat for endangered species often involves translocation of seeds or individuals from source populations to an area targeted for revegetation. Long-term persistence of a species is dependent on the maintenance of sufficient genetic variation within and among populations. Thus, knowledge and maintenance of genetic variability within rare or endangered species is essential for developing effective conservation and restoration strategies. Genetic monitoring of both natural and restored populations can provide an assessment of restoration protocol success in establishing populations that maintain levels of genetic diversity similar to those in natural populations. California’s vernal pools are home to many endangered plants, thus conservation and restoration are large components of their management. Lasthenia conjugens (Asteraceae) is a federally endangered self-incompatible vernal pool annual with gravity- dispersed seeds. Using the molecular technique of intersimple sequence repeats (ISSRs), this study assessed levels and patterns of genetic variability present within natural and restored populations of L. conjugens. At Travis Air Force Base near Fairfield, California, a vernal pool restoration project is underway. Genetic success of the ecologically based seeding protocol was examined through genetic monitoring of natural and restored populations over a three-year period. Genetic diversity remained constant across the three sampled generations. Diversity was also widely distributed across all populations. We conclude that the protocol used to establish restored populations was successful in capturing similar levels and patterns of genetic diversity to those seen within natural pools. This study also demonstrates how genetic markers can be used to inform conservation and restoration decisions.


conservation ISSR Lasthenia restoration genetics vernal pools 


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We thank Yan Linhart, Andy Martin, and Susan Beatty for valuable comments on the manuscript. This study was funded by grants from the National Science Foundation (DEB-0206088), the Department of Ecology and Evolutionary Biology at the University of Colorado, the University of Colorado Graduate School, the University of Colorado Museum, and the California Native Plant Society. All plant samples were collected under a U.S. Fish and Wildlife permit to SK Collinge (permit TE828382-2). We thank the National Center for Genetic Resources Preservation in Fort Collins, Colorado for allowing J. M. Ramp to use their facilities for DNA extractions. In addition, we thank two undergraduates, Brian Lee and Henry Houghton, who volunteered their time to help with DNA extractions.


  1. Baskin Y (1994) California’s ephemeral vernal pools may be a good model for speciation. BioScience 44: 384–388CrossRefGoogle Scholar
  2. Biosystems Analysis (1994). Vernal pool resources at Travis Air Force Base, Solano County, California: final report. Biosystems Analysis Inc., Tiburon, CAGoogle Scholar
  3. Black C, Zedler PH (1998) An overview of 15 years of vernal pool restoration and construction activities in San Diego County, California. In: Witham CW, Bauder ET, Belk D, Ferren WRJ, Ornduff R (eds) Ecology, Conservation, and Management of Vernal Pool Ecosystems - Proceedings from a 1996 conference. California Native Plant Society, Sacramento, CA, pp. 195–205Google Scholar
  4. Bussell JD (1999) The distribution of random amplified polymorphic DNA (RAPD) diversity amongst population of Isotoma petraea (Lobeliaceae). Mol. Ecol. 8: 775–789CrossRefGoogle Scholar
  5. Chan R (2001) A new section in the Goldfield genus Lasthenia (Compositae: Heliantheae sensu lato). Madrono 48: 38–39Google Scholar
  6. Culley TM, Wolfe AD (2001) Population genetic structure of the cleistogamous plant species Viola pubescens Aiton (Violaceae), as indicated by allozyme and ISSR molecular markers. Heredity 86: 545–556PubMedCrossRefGoogle Scholar
  7. Dawson IK, Simons AH, Waugh R, Powell W (1995) Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays. Heredity 75: 10–18Google Scholar
  8. Desrochers AM, Dodge B (2003) Phylogenetic relationships in Lasthenia (Heliantheae: Asteraceae) based on nuclear rDNA internal transcribed spacer (ITS) sequence data. Sys. Bot. 28: 208–215Google Scholar
  9. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phyto. Bul. 19: 11–15Google Scholar
  10. Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distance among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479–491PubMedGoogle Scholar
  11. Falk DA, Holsinger KE (1991) Genetics and Conservation of Rare Plants. Oxford University Press, New YorkGoogle Scholar
  12. Ferren WRJ, Hubbard DM, Wiseman S, Parikh AK, Gale N (1998) Review of ten years of vernal pool restoration and creation in Santa Barbara, California. In: Witham CW, Bauder ET, Belk D, Ferren WRJ, Ornduff R (eds) Ecology, conservation, and management of vernal pool ecosystems - Proceedings from a 1996 conference. California Native Plant Society, Sacramento, CA, pp. 206–216Google Scholar
  13. Fischer M, Hock M, Paschke M (2003) Low genetic variation reduces cross-compatibility and offspring fitness in populations of a narrow endemic plant with a self-incompatibility system. Conserv. Genet. 4: 325–336CrossRefGoogle Scholar
  14. Fisher RA (1930) The fundamental theorem of natural selection. In: The Genetical Theory of Natural Selection, pp. 22–47. Clarendon Press, Oxford.Google Scholar
  15. Fleishman E, Launer AE, Switky KR, Yandell U, Heywood J, Murphy DD (2001) Rules and exceptions in conservation genetics: genetic assessment of the endangered plant Cordylanthus palmatus and its implications for management planning. Biol. Conserv. 98: 45–53CrossRefGoogle Scholar
  16. Frankham R (1995) Conservation genetics. Ann. Rev. Genet. 29: 305–327PubMedCrossRefGoogle Scholar
  17. Gemmill CEC, Ranker TA, Ragone D, Perlman SP, Wood KR (1998) Conservation genetics of the endangered endemic Hawaiian genus Brighamia (Campanulaceae). Amer. J. Bot. 85: 528–539CrossRefGoogle Scholar
  18. Gerhardt F, Collinge SK (2003) Exotic plant invasions of vernal pools in the Central Valley of California, USA. J. Biogeo. 30:1043–1052Google Scholar
  19. Godwin ID, Aitken EAB, Smith LW (1997) Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis 18: 1524–1528PubMedCrossRefGoogle Scholar
  20. Gustafson DJ, Gibson DJ, Nickrent DL (2002) Genetic diversity and competitive abilities of Dalea purpurea (Fabaceae) from remnant and restored grasslands. Int. J. of Plant Sci. 163: 979–990CrossRefGoogle Scholar
  21. Gustafson DJ, Gibson DJ, Nickrent DL (2004) Conservation genetics of two co-dominant grass species in an endangered grassland ecosystem. J. Appl. Ecol. 41: 389–397CrossRefGoogle Scholar
  22. Helenurm K, Parsons LS (1997) Genetic variation and reintroduction of Cordylanthus maritimus ssp. maritimus to Sweetwater Marsh, California. Restor. Ecol. 5: 236–244CrossRefGoogle Scholar
  23. Hollingsworth PM, Tebbitt M, Watson KJ, Gornall RJ (1998) Conservation genetics of an arctic species, Saxifraga rivularis L., in Britain. Biol. J. Lin.n Soc. 123: 1–14Google Scholar
  24. Jain A, Apparanda C, Bhalla PL (1999) Evaluation of genetic diversity and genome fingerprinting of Pandorea (Bignoniaceae) by RAPD and inter-SSR PCR. Genome 42: 714–719CrossRefGoogle Scholar
  25. Jain SK (1976) Vernal pools: their ecology and conservation. University of California, Davis, CAGoogle Scholar
  26. Lai J-A, Yang W-C, Hsiao J-Y (2001) An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR markers. Bot. Bull. Acad. Sin. 42: 93–100Google Scholar
  27. Lewontin RC (1972) The apportionment of human diversity. Evol. Biol. 6: 381–398Google Scholar
  28. Li Y-Y, Chen X-Y, Zhang X, Wu T-Y, Lu H-P, Cai Y-W (2005) Genetic differences between wild and artificial populations of Metasequoia glyptostroboides: implications for species recovery. Conserv. Biol. 19: 224–231CrossRefGoogle Scholar
  29. Lopez-Pujol J, Bosch M, Simon J, Blanche C (2003) Population genetics and conservation priorities for the critically endangered island endemic Delphinium pentagynum subsp. formenteranum (Ranunculaceae). Biodivers. Conserv. 12: 1937–1951CrossRefGoogle Scholar
  30. Lynch M, Milligan BG (1994) Analysis of population genetic structure with RAPD markers. Mol. Ecol. 3: 91–99PubMedGoogle Scholar
  31. Mattner J, Zwako G, Rossetto M, Krauss SL, Dixon KW, Sivasithamparam K (2002) Conservation genetics and implications for restoration of Hemigenia exilis (Lamiaceae), a serpentine endemic from Western Australia. Biol. Conserv. 107: 37–45CrossRefGoogle Scholar
  32. McGlaughlin M, Karoly K, Kaye T (2002) Genetic variation and its relationship to population size in reintroduced populations of pink sand verbena, Abronia umbellata subsp. breviflora (Nyctaginaceae). Conserv. Genet. 3: 411–420CrossRefGoogle Scholar
  33. Nei M (1973) Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. 70: 3321–3323PubMedCrossRefGoogle Scholar
  34. Newman D, Pilson D (1997) Increased probability of extinction due to decreased genetic effective populations size: experimental populations of Clarkia pulchella. Evolution 51: 354–362CrossRefGoogle Scholar
  35. Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol. Ecol. 13: 1143–1155PubMedCrossRefGoogle Scholar
  36. Nybom H, Bartish IV (2000) Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Persp. Plant Ecol., Evol. Sys. 3: 93–114CrossRefGoogle Scholar
  37. Ornduff R (1966) A biosystematic survey of the Goldfield genus␣Lasthenia. In: University of California Publications in BotanyGoogle Scholar
  38. Pavlik BM, Nickrent DL, Howald AM (1993) The recovery of an endangered plant. I. Creating a new population of Amsinckia grandiflora. Conserv. Biol. 7: 510–526CrossRefGoogle Scholar
  39. Qian W, Ge S, Hong D-Y (2001) Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers. Theor. Appl. Genet. 102: 440–449CrossRefGoogle Scholar
  40. Ramp JM (2004) Restoration genetics and pollination of the endangered vernal pool endemic Lasthenia conjugens (Asteraceae). University of Colorado, Boulder, USA, PhD thesisGoogle Scholar
  41. Schneider S, Roessli D, Excoffier L (2000) Arlequin ver. 2.000: A software program for population genetic data analysis. Genetics and Biometry Laboratory, GenevaGoogle Scholar
  42. Society for Ecological Restoration Working Group (2002) The SER primer on ecological restorationGoogle Scholar
  43. Stewart CN, Jr., Excoffier L (1996) Assessing population genetic structure and variability with RAPD data: application to Vaccinium macrocarpon (American Cranberry). J. Evol. Biol. 9: 153–171CrossRefGoogle Scholar
  44. Stone DR (1990) California’s endemic vernal pool plants: some factors influencing their rarity and endangerment. In: Ikeda DH, Schlising RA (eds) Vernal Pool plants - their habitat and biology. California State University, Studies from the Herbarium No. 8, California State University, Chico, pp. 89–107Google Scholar
  45. Thorp RW (1976) Insect pollination of vernal pool flowers. In: Jain SK (ed) Vernal Pools: Their Ecology and Conservation. Institute of Ecology, University of California, Davis, pp. 36–40Google Scholar
  46. Thorp RW (1990) Vernal pool flowers and host-specific bees. In: Ikeda DH, Schlising RA (eds) Vernal Pool plants - their habitat and biology. California State University, Studies from the Herbarium No. 8, California State University, Chico, pp. 109–122Google Scholar
  47. Travis SE, Proffitt CE, Lowenfeld RC, Mitchell TW (2002) A comparative assessment of genetic diversity among differently-aged populations of Spartina alterniflora on restored versus natural wetlands. Restor. Ecol. 10: 37–42CrossRefGoogle Scholar
  48. United States Fish and Wildlife Service (1997) Endangered and threatened wildlife and plants; Endangered status for four plants from vernal pools and mesic areas in Northern California. Federal Register 62: 33029–33038Google Scholar
  49. United States Fish and Wildlife Service (2003) Endangered and threatened wildlife and plants; Final designation of critical habitat for four vernal pool crustaceans and eleven vernal pool plants in California and Southern Oregon. Federal Register 68: 46732–46782Google Scholar
  50. Williams SL, Davis CA (1996) Population genetic analyses of transplanted Eelgrass (Zostera marina) beds reveal reduced genetic diversity in southern California. Restor. Ecol. 4: 163–180CrossRefGoogle Scholar
  51. Williams SL, Orth RJ (1998) Genetic diversity and structure of natural and transplanted Eelgrass populations in the Chesapeake and Chincoteague Bays. Estuaries 21: 118–128CrossRefGoogle Scholar
  52. Wise CA, Ranker TA, Linhart YB (2002) Modeling problems in conservation genetics with Brassica rapa: Genetic variation and fitness in plants under mild, stable conditions. Conserv. Biol. 16: 1542–1554CrossRefGoogle Scholar
  53. Wolfe AD, Liston A (1998) Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis DE, Soltis PS, Doyle JJ, (eds) Molecular Systematics of Plants II DNA Sequencing. Kluwer Academic Pub., Boston, pp. 43–86Google Scholar
  54. Wolfe AD, Randle CP (2001) Relationships within and among species of the holoparasitic genus Hyobanche (Orobanchaceae) inferred from ISSR banding patterns and nucleotide sequences. Sys. Bot. 26: 120–130Google Scholar
  55. Yeh FC, Boyle T, Rongcai Y, Ye Z, Xiyan JM (1999). POPGENE Version 1.31. Edmonton, AlbertaGoogle Scholar
  56. Zedler PH (1990). Life histories of vernal pool vascular plants. In: Ikeda DH, Schlising RA (eds) Vernal pool plants - their habitat and biology. California State University, Studies from the Herbarium No. 8, California State University,Chico, pp. 123–146Google Scholar
  57. Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-Anchored polymerase chain reaction amplification. Genomics 20: 176–183PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Jennifer M. Ramp
    • 1
    • 3
  • Sharon K. Collinge
    • 1
    • 2
  • Tom A. Ranker
    • 1
    • 3
  1. 1.Department of Ecology and Evolutionary BiologyUniversity of Colorado BoulderBoulderUSA
  2. 2.Environmental Studies ProgramUniversity of Colorado at BoulderBoulderUSA
  3. 3.University of Colorado Museum, University of Colorado at BoulderBoulderUSA

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