Conservation Genetics

, Volume 6, Issue 4, pp 615–621

Spatial and temporal genetic differentiation and effective population size of brown trout (Salmo trutta, L.) in small Danish rivers

  • Lasse F. Jensen
  • Michael M. Hansen
  • Jens Carlsson
  • Volker Loeschcke
  • Karen-Lise D. Mensberg
Article

Abstract

The spatial and temporal genetic structure of brown trout populations from three small tributaries of Lake Hald, Denmark, was studied using analysis of variation at eight microsatellite loci. From two of the populations temporal samples were available, separated by up to 13 years (3.7 generations). Significant genetic differentiation was observed among all samples, however, hierarchical analysis of molecular variance (AMOVA) showed that differentiation among populations accounted for a non-significant amount of the genetic differentiation, whereas differentiation among temporal samples within populations was highly significant (0.0244, P<0.001). Estimates of effective population size (Ne) using a maximum-likelihood based implementation of the temporal method, yielded small values (Ne ranging from 33 to 79). When a model was applied that allows for migration among populations, Ne estimates were even lower (24–54), and migration rates were suggested to be high (0.13–0.36). All samples displayed a clear signal of a recent bottleneck, probably stemming from a period of unfavourable conditions due to organic pollution in the 1970–1980’s. By comparison to other estimates of Ne in brown trout, Lake Hald trout represent a system of small populations linked by extensive gene flow, whereas other populations in larger rivers exhibit much higher Ne values and experience lower levels of immigration. We suggest that management considerations for systems like Lake Hald brown trout should focus both on a regional scale and at the level of individual populations, as the future persistence of populations depends both on maintaining individual populations and ensuring sufficient migration links among these populations.

Key words

effective population size gene flow microsatellite DNA Salmo trutta spatio-temporal genetic structure 

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Lasse F. Jensen
    • 1
    • 2
  • Michael M. Hansen
    • 2
  • Jens Carlsson
    • 3
  • Volker Loeschcke
    • 1
  • Karen-Lise D. Mensberg
    • 2
  1. 1.Department of Genetics and EcologyUniversity of AarhusNy MunkegadeDenmark
  2. 2.Department of Inland FisheriesDanish Institute for Fisheries ResearchVejlsøvej 39Denmark
  3. 3.Virginia Institute of Marine Science, School of Marine Science, College of William and MaryGloucester PointUSA

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