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Clinical & Experimental Metastasis

, Volume 30, Issue 5, pp 651–658 | Cite as

Decreased expression of miR-126 correlates with metastatic recurrence of hepatocellular carcinoma

  • Hongyuan Chen
  • Ruizheng Miao
  • Junwei Fan
  • Zhongbo Han
  • Junyi Wu
  • Guoqiang Qiu
  • Huamei Tang
  • Zhihai PengEmail author
Research Paper

Abstract

The liver transplant (LT) situation represents an attractive model for studying hepatocellular carcinoma (HCC) metastasis. Based on microarray data, we previously found that miR-126 expression was lower in tumor tissues of patients with post-LT HCC recurrence compared with non-recurrence. In this study, we examined the expression of miR-126 in HCC samples from 68 patients who had undergone LT using quantitative real-time PCR and analyzed its correlation with clinicopathological features and prognosis of patients. Furthermore, we performed experimental analyses to explore the involvement of miR-126 in HCC metastasis. We found that miR-126 levels were lower in tumor tissues of patients with post-LT HCC recurrence in comparison to patients with no-recurrence (p = 0.009). Lower expression of miR-126 in HCC was associated significantly with tumor recurrence (p = 0.011) and poor survival (p = 0.009) of patients. Functional studies indicated that ectopic expression of miR-126 significantly inhibits HCC cells migration, invasion, proliferation and colony formation in vitro, and suppresses experimental lung colonization in vivo. Our study revealed that down-regulation of miR-126 plays an important role in HCC metastasis, and suggest a potential application of miR-126 in prognosis prediction and HCC treatment.

Keywords

miR-126 Hepatocellular carcinoma Liver transplantation Metastasis 

Abbreviations

HCC

Hepatocellular carcinoma

LT

Liver transplant

miR(s)

MicroRNA(s)

qRT-PCR

Quantitative real time-polymerase chain reaction

FFPE

Formalin-fixed paraffin embedded

MET

Mesenchymal epithelial transition

OS

Overall survival

Notes

Acknowledgments

This study was supported by National Key Technology R&D Program (2008BAI60B03).

Conflict of interest

The authors disclosed no commercial or financial interest in the subject of study.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Hongyuan Chen
    • 1
  • Ruizheng Miao
    • 2
  • Junwei Fan
    • 1
  • Zhongbo Han
    • 3
  • Junyi Wu
    • 1
  • Guoqiang Qiu
    • 1
  • Huamei Tang
    • 4
  • Zhihai Peng
    • 1
    Email author
  1. 1.Department of General SurgeryShanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong UniversityShanghaiChina
  2. 2.Department of General SurgeryProvincial Hospital Affiliated To Shandong UniversityJinanChina
  3. 3.Department of General SurgeryCentral Hospital of Zi BoZi BoChina
  4. 4.Department of PathologyShanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong UniversityShanghaiChina

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