Clinical & Experimental Metastasis

, Volume 29, Issue 8, pp 939–948 | Cite as

CD44+/CD24− ovarian cancer cells demonstrate cancer stem cell properties and correlate to survival

  • Erhong Meng
  • Beverely Long
  • Paula Sullivan
  • Steve McClellan
  • Michael A. Finan
  • Eddie Reed
  • Lalita Shevde
  • Rodney P. Rocconi
Research Paper

Abstract

Cancer cells with the surface marker profile CD44+/CD24− have previously been described to possess cancer stem cell-like properties. This manuscript evaluates those properties in ovarian cancer cell lines. The proportion of CD44+/CD24− cells corresponded to the clinical aggressiveness of each ovarian cancer cell line histologic subtype. CD44+/CD24− cells demonstrated enhanced progressive differentiation as well as showing a 60-fold increase in Matrigel invasion in both SKOV3 and OV90 cell lines (p < 0.001 each) compared to other phenotypes. CD44+/CD24− demonstrated significant resistance to all chemotherapy agents used in all cell lines, with a 71–93 % increase in resistance compared with baseline. Using a threshold of 25 % CD44+/CD24– ovarian cancer cells found in ascites, patients with >25 % CD44+/CD24− were significantly more likely to recur (83 vs. 14 %, p = 0.003) and had shorter median progression-free survival (6 vs. 18 months, p = 0.01). In conclusion, the CD44+/CD24− phenotype in ovarian cancer cells demonstrate cancer stem cell-like properties of enhanced differentiation, invasion, and resistance to chemotherapy. This CD44+/CD24− phenotype correlates to clinical endpoints with increased risk of recurrence and shorter progression-free survival in patients with ovarian cancer.

Keywords

CD44+/CD24− Ovarian cancer Cancer stem-like cells Chemoresistance Survival 

Abbreviations

bFGF

Basic fibroblast growth factor

BSA

Bovine serum albumin

CK7

Cytokeratin 7

EGF

Epidermal growth factor

FACS

Fluorescence-activated cell sorting

FCM

Flow cytometry

FITC

Fluorescein isothiocyanate

PFS

Progression free survival

Notes

Acknowledgments

This work was supported by the Gynecologic Cancer Foundation Ovarian Cancer Research Award: Sherri’s From a Whisper to a Roar, Women’s Motorcycle Foundation Award.

Conflict of interest

The authors declare that they have no conflict of interests to disclose.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Erhong Meng
    • 1
  • Beverely Long
    • 1
  • Paula Sullivan
    • 1
  • Steve McClellan
    • 1
  • Michael A. Finan
    • 1
  • Eddie Reed
    • 1
  • Lalita Shevde
    • 1
  • Rodney P. Rocconi
    • 1
  1. 1.University of South Alabama, Mitchell Cancer InstituteMobileUSA

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