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Clinical & Experimental Metastasis

, Volume 27, Issue 3, pp 185–196 | Cite as

In vitro metastatic colonization of human ovarian cancer cells to the omentum

  • Shaheena M. Khan
  • Holly M. Funk
  • Sophie Thiolloy
  • Tamara L. Lotan
  • Jonathan Hickson
  • Gail S. Prins
  • Angela F. Drew
  • Carrie W. Rinker-SchaefferEmail author
Research Paper

Abstract

Despite the potentially crucial contributions of the omentum in the regulation of ovarian cancer metastatic growth, it remains a poorly understood organ. Due to its anatomic location and structural fragility, the omentum presents inherent challenges to mechanism-based in vivo studies. Thus, the availability of an ex vivo omental model would, in part, address some of these difficulties posed. Here we describe a technique for identifying, isolating and maintaining ex vivo cultures of omenta from immune-compromised and -competent mice. Ex vivo culture conditions were developed that maintain tissue viability, architecture, and function for up to 10 days. Further experiments demonstrate that the ex vivo culture conditions allow for the proliferation of ovarian cancer cells in vitro and support a similar pattern of microscopic lesions after either intraperitoneal injection of ovarian cancer cells or co-culture of ovarian cancer cells with the omentum. In agreement with previous studies from our laboratory, histologic evaluation of these specimens found that ovarian cancer cells, as well as other peritoneal cancer cells, preferentially accumulate in, and colonize, omental areas rich in immune cells. We now recognize that these are specific, functional structures referred to as milky spots. In sum, these are foundational studies of a readily accessible model, which is easily manipulated and can be immediately used to study the dynamic process of omental colonization. It is hoped that investigators will use the data herein as a starting point for refinements and modifications which will enable them to tailor the model to the specific needs of the experimental question(s) they wish to pursue.

Keywords

Omentum Ovarian cancer Metastatic colonization Milky spots Immune aggregates 

Abbreviations

H&E

Hematoxylin and eosin

PBS

Phosphate buffered saline

CO2

Carbon dioxide

FCS

Fetal calf serum

GFP

Green fluorescent protein

ITS

Insulin transferrin and selenium solution

dpi

Days post injection

Notes

Acknowledgments

We would like to thank Ms. Jennifer Taylor for her support and assistance in the conducting the intraperitoneal injections and for generously providing the SKOV3ip.1-GFP tagged cells for use in this study. We appreciate the supportive and enthusiastic input of Dr. Karl Matlin and Dr. Jerry Turner of the University of Chicago as we developed work reported herein. We appreciate the superb technical support and assistance of Dr. Lynnette Gerhold of the University of Chicago Optical Imaging Core Facility and Ms. Shirley Bond of the University of Chicago Biological Sciences Division Microscopy Core Facility. This work was made possible by the generous philanthropic support of The University of Chicago Section of Urology and grants from The Department of Defense Ovarian Cancer Research Program (W81XWH-09-0127) and the National Cancer Institute (2R01CA089569-06A2).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Shaheena M. Khan
    • 1
    • 2
  • Holly M. Funk
    • 3
  • Sophie Thiolloy
    • 1
  • Tamara L. Lotan
    • 4
  • Jonathan Hickson
    • 5
  • Gail S. Prins
    • 6
  • Angela F. Drew
    • 3
  • Carrie W. Rinker-Schaeffer
    • 1
    • 2
    • 7
    Email author
  1. 1.Department of Surgery, Section of Urology MC6038The University of ChicagoChicagoUSA
  2. 2.Department of PathologyThe University of ChicagoChicagoUSA
  3. 3.Department of Cancer and Cell BiologyUniversity of CincinnatiCincinnatiUSA
  4. 4.Department of PathologyThe Johns Hopkins University School of MedicineBaltimoreUSA
  5. 5.Abbot LaboratoriesAbbot ParkUSA
  6. 6.Department of UrologyThe University of Illinois at ChicagoChicagoUSA
  7. 7.Departments of Obstetrics and Gynecology and MedicineThe University of ChicagoChicagoUSA

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