Clinical & Experimental Metastasis

, Volume 22, Issue 1, pp 85–93 | Cite as

Frequent loss of membranous E-cadherin in gastric cancers: A cross-talk with Wnt in determining the fate of β-catenin

  • Xiao-Xin Cheng
  • Zi-Chuang Wang
  • Xiao-Yan Chen
  • Yuan Sun
  • Qing-You Kong
  • Jia Liu
  • Xue Gao
  • Hong-Wei Guan
  • Hong Li
Article

Abstract

The potential correlation of E-cadherin reduction and Wnt2 up-regulation in determining the intracellular distribution of β-catenin in gastric cancers was investigated by the methods of frozen tissue array-based immunohistochemistry, Western blot and RT-PCR analysis. It was revealed that membranous E-cadherin was reduced frequently in the two major subtypes of gastric cancer (intestinal gastric cancer, i-GC and diffuse gastric cancer, d-GC) and closely correlated with the risk of lymphoid node metastasis (P < 0.05). The reduction of membranous E-cadherin was paralleled with cytosolic and nuclear accumulation of β-catenin and the increased Wnt2 expression. These results indicate that the reduced E-cadherin is a common genetic phenotype of GCs and plays beneficial roles in tumor metastasis. Altered β-catenin distribution may result from the imbalance of E-cadherin production and Wnt expression, which confers on gastric cancer cells more aggressive behaviors.

Keywords

β-catenin E-cadherin gastric cancer metastasis Wnt2 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Xiao-Xin Cheng
    • 1
  • Zi-Chuang Wang
    • 1
  • Xiao-Yan Chen
    • 1
  • Yuan Sun
    • 1
  • Qing-You Kong
    • 1
  • Jia Liu
    • 1
  • Xue Gao
    • 1
    • 2
  • Hong-Wei Guan
    • 2
  • Hong Li
    • 1
  1. 1.Cancer Institute and The Liaoning Provincial Key Laboratory of Cancer Genomics, College of Basic Medical SciencesDalian Medical UniversityDalianP. R. China
  2. 2.Division of Pathology, First Affiliated HospitalDalian Medical UniversityDalianP. R. China

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