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Clinical & Experimental Metastasis

, Volume 22, Issue 2, pp 99–106 | Cite as

Role of the β1-integrin subunit in the adhesion, extravasation and migration of T24 human bladder carcinoma cells

  • Christoph Heyder
  • Eva Gloria-Maercker
  • Wolfgang Hatzmann
  • Bernd Niggemann
  • Kurt S. Zänker
  • Thomas Dittmar
Article

Abstract

The abilities of tumor cells to extravasate from the blood vessel system and to migrate through the connective tissue are prerequisites in metastasis formation. Both processes are chiefly mediated by integrins, which mediate both cell–cell and cell–matrix interactions. We investigated the role of integrin subunits in the adhesion, extravasation and migration of the highly invasive human bladder carcinoma cell line T24. Here we show that inhibition of the β1-integrin subunit using the specific β1-integrin blocking antibody 4B4 significantly reduces the adhesion to HUVEC and transmigratory activity of T24 cells. The blockade of the β1-integrin subunit also resulted in a significantly reduced locomotory activity of T24 cells. A detailed cell migration analysis on a single cell level revealed that blockade of the β1-integrin subunit leads to an altered migration pattern of single cells but does not influence migration per se. Migration parameters such as time active, velocity and distance migrated were significantly reduced as compared to untreated control cells. Our observations strongly suggest a central role for the β1-integrin subunit in forming the cell–cell and cell–matrix bonds necessary for adhesion, extravasation and migration.

Keywords

adhesion β1-integrin cell migration extravasation metastasis 

Abbreviations

FCS

fetal calf serum

HUVEC

human umbilical vein endothelial cell

IL-8

Interleukin-8

XTT

(2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]2H-tretrazolium-5-carboxanilide) sodium salt

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Copyright information

© Springer 2005

Authors and Affiliations

  • Christoph Heyder
    • 1
  • Eva Gloria-Maercker
    • 1
  • Wolfgang Hatzmann
    • 2
  • Bernd Niggemann
    • 1
  • Kurt S. Zänker
    • 1
  • Thomas Dittmar
    • 1
  1. 1.Institute of ImmunologyWitten/Herdecke UniversityWittenGermany
  2. 2.Department of Gynecology, Marienhospital WittenUniversity of Witten/HerdeckeWittenGermany

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