Clinical & Experimental Metastasis

, Volume 21, Issue 8, pp 685–697 | Cite as

Ovarian carcinoma spheroids disaggregate on type I collagen and invade live human mesothelial cell monolayers

  • Kathryn M. Burleson
  • Linda K. Hansen
  • Amy P. N. Skubitz
Article

Abstract

Ovarian carcinoma patients frequently develop malignant ascites containing single and aggregated tumor cells, or spheroids. Spheroids have been shown to be resistant to many therapies, but their contribution to ovarian cancer dissemination remains undetermined. We have previously shown that ascites spheroids adhere to extracellular matrix (ECM) proteins and live human mesothelial cells via β1 integrin subunits. Here, we assessed the ability of spheroids that were generated from the human ovarian carcinoma cell line NIH: OVCAR5 to disseminate and invade in vitro. Spheroids were seeded on ECM proteins for 24 h. While laminin and type IV collagen stimulated some cell migration, spheroids completely disaggregated on type I collagen substrates. A monoclonal antibody against the β1 integrin subunit significantly inhibited disaggregation on all proteins tested. To test their invasive ability, spheroids were added to monolayers of live human LP9 mesothelial cells. Within 24 h, the spheroids adhered and disaggregated on top of the monolayers, and within a week had established foci of invasion encompassing a 200-fold larger surface area. Addition of a monoclonal antibody against the β1 integrin subunit drastically reduced spheroid invasion into the mesothelial cell monolayers. GM 6001, a broad-scale matrix metalloproteinase inhibitor, also significantly blocked spheroid invasion into the mesothelial cell monolayers. ɛ-amino-N-caproic acid, a serine protease inhibitor, partially inhibited spheroid invasion. Based on their ability to attach to, disaggregate on, and invade into live human mesothelial cell monolayers, spheroids should thus be regarded as potential contributors to the dissemination of ovarian cancer.

Keywords

cell adhesion molecules extracellular matrix integrins ovarian carcinoma spheroids 

Abbreviations

BSA

bovine serum albumin

DMSO

dimethyl sulfoxide

ECM

extracellular matrix

EDTA

ethylenediaminetetraacetic acid

EHS

Engelbreth–Holm–Swarm

FBS

fetal bovine serum

mAb

monoclonal antibody

mIgG

normal mouse immunoglobulin

MMP

matrix metalloproteinase

PBS

phosphate buffered saline

ɛACA

ɛ–amino-n-caproic acid

uPA

urinary-type plasminogen activator

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Copyright information

© Springer 2005

Authors and Affiliations

  • Kathryn M. Burleson
    • 1
  • Linda K. Hansen
    • 1
  • Amy P. N. Skubitz
    • 1
  1. 1.Department of Laboratory Medicine and PathologyUniversity of MinnesotaMinneapolisUSA

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