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Clinical & Experimental Metastasis

, Volume 21, Issue 7, pp 571–579 | Cite as

Constitutive expression of growth regulated oncogene (gro) in human colon carcinoma cells with different metastatic potential and its role in regulating their metastatic phenotype

Article

Abstract

The purpose of this study was to examine the expression and functional significance of the growth-regulated oncogene (gro) family in human colon carcinoma growth and metastasis. We examined constitutive expression of CXCL1 (gro-α), CXCL2 (gro-β), CXCL3 (gro-γ) and their receptor, CXCR2 in human colon carcinoma cells with different metastatic potentials. Non-metastatic and low metastatic cells expressed lower levels of CXCL1 and CXCR2 mRNA and protein as compared to high metastatic colon carcinoma cells. No difference in CXCL2 and CXCL3 mRNA expression levels was observed. Colon carcinoma cells expressing higher levels of CXCL1 exhibit increased proliferation and invasive potential. Furthermore, exogenous addition of recombinant human CXCL1 significantly enhanced the proliferation and invasiveness of colon carcinoma cells. Furthermore, treatment of KM12C cells with exogenous CXCL1 enhanced their invasiveness. Neutralizing antibody to CXCL1 in combination with antibody to CXCR2 inhibited highly metastatic KM12L4 (high CXCL1 expressor) cell proliferation. These data demonstrate that the constitutive expression of CXCL1 and its receptor CXCR2 is associated with metastatic potential and modulates colon cancer cell proliferation and an invasive phenotype.

Keywords

chemokines colon cancer CXCL1-3 CXCR1-2 

Abbreviations

ELISA

enzyme-linked immunosorbent assay

FBS

fetal bovine serum

gro

growth regulated oncogene

IHC

immunohistochemistry

RT-PCR

reverse transcriptase-polymerase chain reaction

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Copyright information

© Springer 2005

Authors and Affiliations

  • Aihua Li
    • 1
  • Michelle L. Varney
    • 1
  • Rakesh K. Singh
    • 1
  1. 1.Department of Pathology and MicrobiologyThe University of Nebraska Medical Center, Nebraska Medical CenterOmahaUSA

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