Clinical & Experimental Metastasis

, Volume 21, Issue 6, pp 543–552 | Cite as

Common origins of MDA-MB-435 cells from various sources with those shown to have melonoma properties

  • James M. Rae
  • Susan J. Ramus
  • Mark Waltham
  • Jane E. Armes
  • Ian G. Campbell
  • Robert Clarke
  • Robert J. Barndt
  • Michael D. Johnson
  • Erik W. Thompson


Recently, the tissue origin of MDA-MB-435 cell line has been the subject of considerable debate. In this study, we set out to determine whether MDA-MB-435-DTP cells shown to express melanoma-specific genes were identical to various other MDA-MB-435 cell stocks worldwide. CGH-microarray, genetic polymorphism genotyping, microsatellite fingerprint analysis and/or chromosomal number confirmed that the MDA-MB-435 cells maintained at the Lombardi Comprehensive Cancer Center (MDA-MB-435-LCC) are almost identical to the MDA-MB-435-DTP cells, and showed a very similar profile to those obtained from the same original source (MD Anderson Cancer Center) but maintained independently (MDA-MB-435-PMCC). Gene expression profile analysis confirmed common expression of genes among different MDA-MB-435-LCC cell stocks, and identified some unique gene products in MDA-MB-435-PMCC cells. RT-PCR analysis confirmed the expression of the melanoma marker tyrosinase across multiple MDA-MB-435 cell stocks. Collectively, our results show that the MDA-MB-435 cells used widely have identical origins to those that exhibit a melanoma-like gene expression signature, but exhibit a small degree of genotypic and phenotypic drift.

MDA-MB-435 breast cancer cell lines melanoma microsatellite analysis chromosomal number tyrosinase 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • James M. Rae
    • 1
  • Susan J. Ramus
    • 1
  • Mark Waltham
    • 1
  • Jane E. Armes
    • 1
  • Ian G. Campbell
    • 1
  • Robert Clarke
    • 1
  • Robert J. Barndt
    • 1
  • Michael D. Johnson
    • 1
  • Erik W. Thompson
    • 1
  1. 1.Department of Internal MedicineUniversity of MichiganAnn ArborUSA

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