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Clinical & Experimental Metastasis

, Volume 21, Issue 6, pp 553–561 | Cite as

Breast adenocarcinoma cell adhesion to the vascular subendothelium in whole blood and under flow conditions: Effects of α v β 3 and α IIb β 3 antagonists

  • Noëlia Gomes
  • Jany Vassy
  • Claude Lebos
  • Brigitte Arbeille
  • Chantal Legrand
  • Françoise Fauvel-Lafeve
Article

Abstract

Tumour cell adhesion to vascular extracellular matrix (ECM), an important step of metastatic progression, is promoted by platelets. The aim of our study was to investigate, in whole blood under venous and arterial shear conditions, the respective role of tumour cell αvβ3 and platelet αIIbβ3integrins in MDA-MB-231 breast adenocarcinoma cell adhesion to human umbilical vein endothelial cell ECM. For that purpose, blood containing MDA-MB-231 cells was incubated with non-peptide antagonists specific for platelet αIIbβ3 (lamifiban) or tumour cell αvβ3 (SB-273005). At 300 s−1, each antagonist used alone did not modify tumour cell adhesion, whereas, at 1500 s−1, tumour cell adhesion was decreased by 25% in presence of lamifiban indicating a role of platelet αIIbβ3 at higher shear rate. However, a combination of SB-273005 and lamifiban, or c7E3 Fab (a potent inhibitor of both αIIbβ3 and αvβ3) inhibited tumour cell adhesion by 40–45%, at either shear rate applied, indicating a cooperation between these two integrins in MDA-MB-231 cell adhesion to ECM, as well as the participation of other adhesive receptors on tumour cells and/or platelets. Thus, efficient anti-metastatic therapy should target multiple receptors on tumour cells and platelets.

adhesion extracellular matrix integrin integrin antagonists platelet shear rate tumour cell 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Noëlia Gomes
    • 1
  • Jany Vassy
    • 1
  • Claude Lebos
    • 1
  • Brigitte Arbeille
    • 1
  • Chantal Legrand
    • 1
  • Françoise Fauvel-Lafeve
    • 1
  1. 1.INSERM, U 553 Hémostase, Endothélium et Angiogénèse, IFR 105, Institut d'HématologieUniversité Paris VII Denis Diderot, Hôpital Saint-Louis 1ParisFrance

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