Clinical & Experimental Metastasis

, Volume 21, Issue 5, pp 445–451 | Cite as

Resveratrol inhibits hepatoma cell invasion by suppressing gene expression of hepatocyte growth factor via its reactive oxygen species-scavenging property



Resveratrol, present in grapes and other plants, is a polyphenolic compound with strong antioxidative activity. In our previous studies, we found that reactive oxygen species (ROS) accelerated the invasive capacity of a rat ascites hepatoma cell line of AH109A in culture and that resveratrol and resveratrol-loaded rat sera suppressed the ROS-potentiated invasion of the hepatoma cells. To study mechanisms by which resveratrol and itsin vivo metabolite(s) suppress the invasion, we estimated intracellular peroxide level and expression of hepatocyte growth factor (HGF), a known cell motility factor, in AH109A cells. Exogenously added ROS promoted the intracellular peroxide level and the expression of HGF. Resveratrol and resveratrol-loaded rat sera canceled the rise in the peroxide level and HGF expression in ROS-stimulated tumor cells. These results suggest an involvement of the antioxidative property of resveratrol and sera from rats orally given resveratrol in their suppressive effects on ROS-potentiated invasion of AH109A cells.


hepatocyte growth factor hepatoma invasion reactive oxygen species resveratrol 



a rat ascites hepatoma cell line of AH109A


carboxymethyl cellulose sodium salt


calf serum


2’,7’-dichlorofluorescin diacetate


dimethyl sulfoxide


enzyme-linked immunosorbent assay


hepatocyte growth factor




50% inhibitory concentration


rat mesentery-derived mesothelial cells


Eagle’s minimum essential medium


phosphate buffered saline


reactive oxygen species


rat serum


xanthine oxidase


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Department of Applied Biological ScienceTokyo Noko UniversityFuchu, TokyoJapan

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