Clinical & Experimental Metastasis

, Volume 21, Issue 5, pp 445–451 | Cite as

Resveratrol inhibits hepatoma cell invasion by suppressing gene expression of hepatocyte growth factor via its reactive oxygen species-scavenging property

Article

Abstract

Resveratrol, present in grapes and other plants, is a polyphenolic compound with strong antioxidative activity. In our previous studies, we found that reactive oxygen species (ROS) accelerated the invasive capacity of a rat ascites hepatoma cell line of AH109A in culture and that resveratrol and resveratrol-loaded rat sera suppressed the ROS-potentiated invasion of the hepatoma cells. To study mechanisms by which resveratrol and itsin vivo metabolite(s) suppress the invasion, we estimated intracellular peroxide level and expression of hepatocyte growth factor (HGF), a known cell motility factor, in AH109A cells. Exogenously added ROS promoted the intracellular peroxide level and the expression of HGF. Resveratrol and resveratrol-loaded rat sera canceled the rise in the peroxide level and HGF expression in ROS-stimulated tumor cells. These results suggest an involvement of the antioxidative property of resveratrol and sera from rats orally given resveratrol in their suppressive effects on ROS-potentiated invasion of AH109A cells.

Keywords

hepatocyte growth factor hepatoma invasion reactive oxygen species resveratrol 

Abbreviations

AH109A

a rat ascites hepatoma cell line of AH109A

CMC

carboxymethyl cellulose sodium salt

CS

calf serum

DCFH-DA

2’,7’-dichlorofluorescin diacetate

DMSO

dimethyl sulfoxide

ELISA

enzyme-linked immunosorbent assay

HGF

hepatocyte growth factor

HX

hypoxanthine

IC50

50% inhibitory concentration

M-cells

rat mesentery-derived mesothelial cells

MEM

Eagle’s minimum essential medium

PBS

phosphate buffered saline

ROS

reactive oxygen species

RS

rat serum

XO

xanthine oxidase

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Department of Applied Biological ScienceTokyo Noko UniversityFuchu, TokyoJapan

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