Czechoslovak Journal of Physics

, Volume 56, Supplement 4, pp D705–D710 | Cite as

The determination of the rate of conjugation immunoglobuline with bifunctional chelator

  • Z. Málek
  • V. Miler
  • F. Budský
Article
  • 21 Downloads

Abstract

The work was performed under the GACR project: „Technology of preparation of radionuclides and their labelled compounds for nuclear medicine and pharmacy with the use of the reactor LVR-15“ reg. no. 104/03/0499. Imaging of cell’s antigens with the use of labelled immunoglobulines allows imaging of specific receptors on cell membrane and specific tumours. It is necessary to carry out the labelling of the immunoglobulines with radionuclides of suitable physical properties, which form cations (e.g., 111In, 90Y, 177Lu) that form very strong chelates of sufficiently high stability constant preventing the dissociation of complexes or the radionuclide under “ in-vivo” conditions. The immunoglobuline must be conjugated with the bifunctional chelator (BCH), which contains both chelating unit and reactive group for binding to the immunoglobuline. In our laboratory we have conjugated human IgG and monoclonal antibody CD20 with diethylenetriamine pentaacetic acid dianhydride (cDTPAA). Radionuclides 90Y and 177Lu prepared on the LVR-15 reactor in NRI Rez were used for labelling. After conjugation and labelling the yields in relation to the amount of isotopic carrier have been determined.

Keywords

Radionuclide DTPA Diethylenetriamine Pentaacetic Acid Labelling Yield Immunoglobuline 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. [1]
    Pressman D.: Ann N.Y. Acad. Sci. 69 (1957) 644.CrossRefGoogle Scholar
  2. [2]
    Cotton F.A. and Wilkinson G. The group element: F, Cl, I, At. In: Advanced Inorganic Chemistry, N.Y. J. Willey, Ed. 3, (1972) p. 458.Google Scholar
  3. [3]
    Hnatowich D.J., Childs R.L., Lanteigne D., and Najafi A.: J. Immunol. Methods 65 (1983) 147.CrossRefGoogle Scholar
  4. [4]
    Budský F., Prokop J., and Hradil M.: Jaderná energie 35 (1988) 142.Google Scholar
  5. [5]
    Budský F., Prokop J., Raban P. and Šeblová J.: Způsob přípravy cyklických anhydridů aminopolykarbonových kyselin, PV 4329/88/1988, AO 272584.Google Scholar
  6. [6]
    Málek Z., Miler V., Budský F., Reková M., and Kronrád L.: Report ÚJV Řež No. 12240 (2005).Google Scholar
  7. [7]
    Miler V., Budský F., and Málek Z.: Report ÚJV Řež No. 11939 (2003).Google Scholar
  8. [8]
    Kysela J., Málek Z., Budský F., and Miler V.: Report ÚJV Řež No. 11774 (2002).Google Scholar

Copyright information

© Institute of Physics, Academy of Sciences of Czech Republic 2006

Authors and Affiliations

  • Z. Málek
    • 1
  • V. Miler
    • 1
  • F. Budský
    • 1
  1. 1.Nuclear Research Institute ŘežŘež near PragueCzech Republic

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