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Chromosome Research

, Volume 27, Issue 4, pp 333–344 | Cite as

Comparative meiosis and cytogenomic analysis in euploid and aneuploid hybrids of Urochloa P. Beauv

  • Mara Jane da Rocha
  • Raquel Bezerra Chiavegatto
  • Ana Gabriela Damasceno
  • Laiane Corsini Rocha
  • Fausto Souza Sobrinho
  • Vânia Helena TechioEmail author
Original Article

Abstract

The genus Urochloa includes most of the important grasses and hybrids currently used as pastures in the tropical regions. Cytogenetic analyzes have identified some aneuploid hybrids that provide new perspectives for genetic breeding. The objective was to analyze the meiotic behavior in euploid (2n = 4x = 36) and aneuploid (2n = 4x = 36 + 2) hybrids of U. ruziziensis x U. decumbens and U. ruziziensis x U. brizantha. Later, the chromosomes and respective genomes involved in pairing configurations and abnormalities were identified through GISH, with an emphasis on tracking the behavior of the additional chromosomes in the aneuploid hybrid U. ruziziensis x U. decumbens (B1B2B2B2 genomes). The aneuploid U. ruziziensis x U. decumbens shows a higher frequency of univalents, reduction of bivalents, and higher index of irregularities compared with the euploid hybrid. For the aneuploid U. ruziziensis x U. brizantha, there was a reduction in the frequency of univalents, an increase in bivalent and trivalent rates and a lower frequency of abnormalities when compared with the euploid hybrid. The rates of meiotic abnormalities and pairing configurations are parental genotype-dependent and influenced by trisomy. The chromosomes of the B1 and B2 genomes of the aneuploid hybrid (U. ruziziensis x U. decumbens) are involved in the formation of univalents, bivalents, and multivalents in inter-, intra- and inter–intragenomic pairings. In general, the segregation times of chromosomes of the genomes are different, since the chromosomes of the B1 genome segregate more slowly.

Keywords

Microsporogenesis Genomic analysis Interspecific hybrids Trisomy Brachiaria 

Abbreviations

GISH

Genomic in situ hybridization

FISH

Fluorescent in situ hybridization

gDNA

Genomic DNA

CTAB

Cetyltrimethylammonium bromide

SSC

2′ saline sodium citrate

DAPI

4′,6-diamidino-2-phenylindole

rDNA

Ribosomal DNA

Notes

Funding information

The authors thank the support of the Foundation for Research Support of the State of Minas Gerais (FAPEMIG), the Coordination for the Improvement of Higher Education Personnel (CAPES), the National Council for Scientific and Technological Development (CNPq) for financial support for the development of this study.

Supplementary material

10577_2019_9616_Fig5_ESM.png (503 kb)
Fig. S1

Mitotic metaphases of the EGL RxD#01 hybrid between U. ruziziensis x U. decumbens with 36 chromosomes (a) and the hybrid 10.6-168 between U. ruziziensis x U. brizantha with 36 + 2 chromosomes (b). The bar represents 10 μm. (PNG 503 kb)

10577_2019_9616_MOESM1_ESM.tif (8.8 mb)
High Resolution Image (TIF 9041 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biology/DBI – Plant Cytogenetics LaboratoryFederal University of Lavras (UFLA)LavrasBrazil
  2. 2.Embrapa Gado de LeiteJuiz de ForaBrazil

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