Genome-wide comparative chromosome maps of Arvicola amphibius, Dicrostonyx torquatus, and Myodes rutilus
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The subfamily Arvicolinae consists of a great number of species with highly diversified karyotypes. In spite of the wide use of arvicolines in biological and medicine studies, the data on their karyotype structures are limited. Here, we made a set of painting probes from flow-sorted chromosomes of a male Palearctic collared lemming (Dicrostonyx torquatus, DTO). Together with the sets of painting probes made previously from the field vole (Microtus agrestis, MAG) and golden hamster (Mesocricetus auratus, MAU), we carried out a reciprocal chromosome painting between these three species. The three sets of probes were further hybridized onto the chromosomes of the Eurasian water vole (Arvicola amphibius) and northern red-backed vole (Myodes rutilus). We defined the diploid chromosome number in D. torquatus karyotype as 2n = 45 + Bs and showed that the system of sex chromosomes is X1X2Y1. The probes developed here provide a genomic tool-kit, which will help to investigate the evolutionary biology of the Arvicolinae rodents. Our results show that the syntenic association MAG1/17 is present not only in Arvicolinae but also in some species of Cricetinae; and thus, should not be considered as a cytogenetic signature for Arvicolinae. Although cytogenetic signature markers for the genera have not yet been found, our data provides insight into the likely ancestral karyotype of Arvicolinae. We conclude that the karyotypes of modern voles could have evolved from a common ancestral arvicoline karyotype (AAK) with 2n = 56 mainly by centric fusions and fissions.
KeywordsComparative cytogenetics Karyotype evolution Voles Lemming
Alexandromys oeconomus (here and further in the text, the species nomenclature is used in accordance with the latest checklist “The mammals of Russia: a taxonomic and geographic reference” (Pavlinov and Lissovsky 2012))
Evolutionary new centromeres
Fluorescence in situ hybridization
G-banding by trypsin using Giemsa
Interstitial telomeric sequences
This study was funded in part by the MCB and SB RAS Programs, research grants of Russian Fund for Basic Research (No. 11-04-00673, No. 14-04-00451 (SAR); No. 14-04-31555 (NAL); No. 15-29-02384, No. 15-04-00962 (ASG)) and ZIN RAS (project No. 01201351185).
Compliance with ethical standards
All institutional and national guidelines for the care and use of laboratory animals were followed.
Conflict of interest
The authors declare that they have no competing of interests.
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