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Chromosome Research

, Volume 23, Issue 3, pp 441–461 | Cite as

Evolutionary dynamics of two satellite DNA families in rock lizards of the genus Iberolacerta (Squamata, Lacertidae): different histories but common traits

  • Verónica Rojo
  • Andrés Martínez-Lage
  • Massimo Giovannotti
  • Ana M. González-Tizón
  • Paola Nisi Cerioni
  • Vincenzo Caputo Barucchi
  • Pedro Galán
  • Ettore Olmo
  • Horacio Naveira
Original Article

Abstract

Satellite DNAs compose a large portion of all higher eukaryotic genomes. The turnover of these highly repetitive sequences is an important element in genome organization and evolution. However, information about the structure and dynamics of reptilian satellite DNA is still scarce. Two satellite DNA families, HindIII and TaqI, have been previously characterized in four species of the genus Iberolacerta. These families showed different chromosomal locations, abundances, and evolutionary rates. Here, we extend the study of both satellite DNAs (satDNAs) to the remaining Iberolacerta species, with the aim to investigate the patterns of variability and factors influencing the evolution of these repetitive sequences. Our results revealed disparate patterns but also common traits in the evolutionary histories of these satellite families: (i) each satellite DNA is made up of a library of monomer variants or subfamilies shared by related species; (ii) species-specific profiles of satellite repeats are shaped by expansions and/or contractions of different variants from the library; (iii) different turnover rates, even among closely related species, result in great differences in overall sequence homogeneity and in concerted or non-concerted evolution patterns, which may not reflect the phylogenetic relationships among taxa. Contrasting turnover rates are possibly related to genomic constraints such as karyotype architecture and the interspersed organization of diverging repeat variants in satellite arrays. Moreover, rapid changes in copy number, especially in the centromeric HindIII satDNA, may have been associated with chromosomal rearrangements and even contributed to speciation within Iberolacerta.

Keywords

Concerted evolution FISH Iberolacerta Library model Satellite DNA Squamate reptiles 

Abbreviations

Cy3

Cyanine 3

dNTP

Deoxyribonucleotide triphosphate

FCA

Factorial correspondence analysis

FISH

Fluorescence in situ hybridization

FITC

Fluorescein iso-thyocianate

Mya

Million years ago

π

Nucleotide diversity

satDNA

Satellite DNA

Notes

Acknowledgments

This work was supported by grants REN2003-02931/GLO (Ministerio de Ciencia y Tecnología, Spain), PGIDIT03RFO10301PR and PGIDIT06RFO10301PR (Xunta de Galicia, Spain) awarded to Horacio Naveira, GRC2014/050 awarded to Ana González, and by grant PRIN2009/20093HYH97 (Ministry of Education, University and Research, Italy) awarded to Vincenzo Caputo Barucchi. Verónica Rojo has been supported by a “FPU” fellowship from Ministerio de Educación, Cultura y Deporte (Spain).

Ethical standards

Permissions for field work and experimental procedures were issued by the competent authorities: Xunta de Galicia (for I. monticola and I. galani), Junta de Castilla y León (for I. cyreni and I. martinezricai), Gobierno de Aragón (for I. bonnali), and Italian Environment Ministry (for I. horvathi). All institutional and national guidelines for the care and use of laboratory animals were followed.

Supplementary material

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Supplementary Fig. 1

Sequence alignment of consensus sequences of the different HindIII (a) and TaqI (b) sequence groups. The first line shows the general consensus for all the sequences of each satDNA. Diagnostic positions for each group are indicated by colored shading. (GIF 743 kb)

10577_2015_9489_Fig7_ESM.gif (1 mb)
Supplementary Fig. 1

Sequence alignment of consensus sequences of the different HindIII (a) and TaqI (b) sequence groups. The first line shows the general consensus for all the sequences of each satDNA. Diagnostic positions for each group are indicated by colored shading. (GIF 743 kb)

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Supplementary Table 1 (DOC 16 kb)
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Supplementary Table 2 (DOC 74 kb)
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Supplementary Table 3 (DOC 46 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Verónica Rojo
    • 1
  • Andrés Martínez-Lage
    • 1
  • Massimo Giovannotti
    • 2
  • Ana M. González-Tizón
    • 1
  • Paola Nisi Cerioni
    • 2
  • Vincenzo Caputo Barucchi
    • 2
    • 3
  • Pedro Galán
    • 4
  • Ettore Olmo
    • 2
  • Horacio Naveira
    • 1
  1. 1.Grupo de Investigación en Bioloxía Evolutiva, Departamento de Bioloxía Celular e MolecularUniversidade da CoruñaA CoruñaSpain
  2. 2.Dipartimento di Scienze della Vita e dell’AmbienteUniversità Politecnica delle MarcheAnconaItaly
  3. 3.Consiglio Nazionale delle RicercheIstituto di Scienze Marine Sezione Pesca MarittimaAnconaItaly
  4. 4.Grupo de Investigación en Bioloxía Evolutiva, Departamento de Bioloxía Animal, Bioloxía Vexetal e EcoloxíaUniversidade da CoruñaA CoruñaSpain

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