Chromosome Research

, Volume 23, Issue 4, pp 753–766 | Cite as

Distribution of histone H4 modifications as revealed by a panel of specific monoclonal antibodies

  • Yoko Hayashi-Takanaka
  • Kazumitsu Maehara
  • Akihito Harada
  • Takashi Umehara
  • Shigeyuki Yokoyama
  • Chikashi Obuse
  • Yasuyuki Ohkawa
  • Naohito Nozaki
  • Hiroshi KimuraEmail author
Open Access


Post-translational histone modifications play a critical role in genome functions such as epigenetic gene regulation and genome maintenance. The tail of the histone H4 N-terminus contains several amino acids that can be acetylated and methylated. Some of these modifications are known to undergo drastic changes during the cell cycle. In this study, we generated a panel of mouse monoclonal antibodies against histone H4 modifications, including acetylation at K5, K8, K12, and K16, and different levels of methylation at K20. Their specificity was evaluated by ELISA and immunoblotting using synthetic peptide and recombinant proteins that harbor specific modifications or amino acid substitutions. Immunofluorescence confirmed the characteristic distributions of target modifications. An H4K5 acetylation (H4K5ac)-specific antibody CMA405 reacted with K5ac only when the neighboring K8 was unacetylated. This unique feature allowed us to detect newly assembled H4, which is diacetylated at K5 and K12, and distinguish it from hyperacetylated H4, where K5 and K8 are both acetylated. Chromatin immunoprecipiation combined with deep sequencing (ChIP-seq) revealed that acetylation of both H4K8 and H4K16 were enriched around transcription start sites. These extensively characterized and highly specific antibodies will be useful for future epigenetics and epigenome studies.


Chromatin Epigenetics Histone modification Monoclonal antibody 



Histone acetyltransferase


Monoclonal antibodies


Phosphate-buffered saline




Transcription start sites


In eukaryotic nuclei, ∼150 bp of DNA is wrapped around the histone octamer, which consists of two copies of four core histones (i.e., H2A, H2B, H3, and H4) to form a nucleosome, the fundamental unit of chromatin. Post-translational modifications on these histones play a critical role in genome function, including the regulation of transcription and the maintenance of genome integrity (Bannister and Kouzarides 2011; Greer and Shi 2012; The ENCODE Project Consortium 2012; Jørgensen et al. 2013). Since H3 and H4 are more stably integrated into nucleosomes compared to H2A and H2B (Kimura and Cook 2001), the modifications on H3 and H4 can act as long-term memory of epigenetic regulation. Among various H3 modifications, trimethylation on H3 lysine 9 and 27 (H3K9me3 and H3K27me3) that is associated with silenced genes can be inherited over cell generations (Martin and Zhang 2005; Greer and Shi 2012; Kimura 2013). Other modifications, such as trimethylation on H3 lysine 4 (H3K4me3) and acetylation are associated with transcriptional activation (Heintzman et al. 2007; Shilatifard 2008; Stasevich et al. 2014). Modifications on histone H4 are also known to be involved in gene regulation and genome maintenance (Shahbazian and Grunstein 2007). Lysine residues in H4 N-terminal tail (i.e., H4K5, H4K8, H4K12, and H4K16) are major acetylation sites (Turner et al. 1989; Johnson et al. 1998; Lang et al. 2013; Taylor et al. 2013; Zheng et al. 2013). These acetylations are predominantly associated with euchromatin, contributing to chromatin decondensation and transcriptional regulation (Turner 1991; Dion et al. 2005; Wang et al. 2008; Bannister and Kouzarides 2011). H4K16ac is also known to be associated with DNA damage repair and cell senescence (Dang et al. 2009; Li et al. 2010; Sharma et al. 2010; Krishnan et al. 2011). In addition, H4K5ac and H4K12ac are associated with newly assembled chromatin since H4 in predeposition complexes is diacetylated at K5 and K12 by a histone acetyltransferase (HAT) (Sobel et al. 1995; Chang et al. 1997). Although the diacetylation of H4 is not a prerequisite for histone assembly (Ma et al. 1998), these modifications may stimulate nuclear import (Ejlassi-Lassallette et al. 2011) and contribute to the recovery from replication block-mediated DNA damage (Barman et al. 2006). After being assembled into chromatin, H4 becomes deacetylated in heterochromatin (Taddei et al. 1999). In contrast to these four lysines that are acetylated, H4 lysine 20 (H4K20) is subject to methylation (Jørgensen et al. 2013). Monomethylation of H4K20 (H4K20me1) may play multiple roles in genome regulation, including transcriptional control, DNA replication licensing, DNA damage response, and chromosome segregation (Wu and Rice 2011; Beck et al. 2012; Kapoor-Vazirani and Vertino 2014). Dimethylation of H4K20 (H4K20me2) is one of the most abundant modifications in mouse fibroblasts and HeLa cells (Pesavento et al. 2008; Schotta et al. 2008), and is involved in DNA damage repair signaling (Greeson et al. 2008).

Trimethylation on H4 lysine 20 (H4K20me3) is also involved in heterochromatin formation, correlated with H3K9me3 (Schotta et al. 2004; Sims et al. 2006). The levels of some histone H4 modifications, like H4K16ac and H4K20me3, are reported to be altered in cancer cells, suggesting these marks can possibly be diagnostic markers (Fraga et al. 2005; Ellis et al. 2009; Rodriguez-Paredes and Esteller 2011; Yokoyama et al. 2014).

To analyze histone modifications, specific antibodies have been valuable tools with a variety of applications, such as chromatin immunoprecipitation, immunoblotting detection, and immunofluorescence (Turner et al. 1992; Peters et al. 2003; Kimura et al. 2008). The quality and reproducibility of these immunochemical analyses rely on antibody specificity and affinity, but commercially available antibodies have not been validated extensively (Clayton et al. 2006; Kimura et al. 2008; Rothbart et al. 2012; Kimura 2013). For example, 20–25 % of histone modification-specific antibodies failed to pass the validation by the ENCODE project (Egelhofer et al. 2011). To facilitate chromatin and epigenetics studies, we have developed histone H3 modification-specific antibodies (Kimura et al 2008; Hayashi-Takanaka et al. 2009, 2011, 2014; Chandra et al. 2012). We here report the generation and validation of a panel of monoclonal antibodies (mAbs) directed against histone H4. These highly reliable and fully characterized mAbs will be useful for future work.

Materials and methods

Ethics statement

All institutional and national guidelines for the care and use of laboratory animals were followed. All animal care and experimental procedures in this study were approved by the Hokkaido University Animal Experiment Committee (approval number: 11-0109) and carried out according to guidelines for animal experimentation at Hokkaido University, where Mab Institute Inc is located. Animals were housed in a specific pathogen-free facility at Hokkaido University. Humane euthanasia of mice was performed by cervical dislocation by individuals with a demonstrated high degree of technical proficiency.

Generation, selection, purification, and dye conjugation of monoclonal antibodies

Synthetic peptides (Sigma-Genosys; Fig. 1) were coupled to keyhole limpet hemocyanin and used to immunize mice (Kimura et al. 1994); after generating hybridomas, clones were screened by ELISA using plates coated with the modified or unmodified peptide conjugated with bovine serum albumin. After recloning, supernatants from clones reacting with the specifically modified peptide were used to probe blots prepared using HeLa total proteins, and the ones giving a single band at the size of histone H4 were selected for immunofluorescence examination to see if they exhibited nuclear staining. Clones that passed through these successive screens were further analyzed by ELISA against peptides listed in Fig. 1, and the ones showing the highest specificity to the authentic peptides were selected. The isotype of each mAb was determined using a kit (Serotec; MMT1; Table 1).
Fig. 1

Specificity of mAbs evaluated by ELISA. Microtiter plates coated with the indicated peptides conjugated with bovine serum albumin were incubated with threefold dilutions of each antibody, starting from 1:100 dilution of a hybridoma culture supernatant. After incubation with peroxidase-conjugated secondary antibody and washing, the colorimetric signal of tetramethylbenzidine was detected by measuring the absorbance at 405 nm using a plate reader. The peptides reacted with the individual mAb are indicated on the panel

Table 1

Summary of monoclonal antibodies








(200a 9C5)








(200Nb 4A7)







(200Nd 72A9)








(200Nf 50B3)








(200f 1B2)





K20- acetyl

K20- methyl



(200b 15F11)







(200c 2E2)







(200d 27F10)






Hybridoma cells were routinely grown in GIT medium (Wako). For antibody purification, cells were grown in CD Hybridoma medium (Life Technologies) and the supernatant (250 ml) was filtrated through a 0.45-μm pore filter, as previously described (Hayashi-Takanaka et al. 2011). For IgG1 subclass mAbs, NaCl was added at a final concentration of 4 M before applying to a HiTrap Protein A HP Sepharose column (1 ml; GE Healthcare), which were pre-equilibrated with Protein A IgG1 binding buffer (Thermo Fisher Scientific). After washing the column with Protein A IgG1 binding buffer, IgG1 was eluted using Mouse IgG1 Mild Elution Buffer (Thermo Fisher Scientific). For IgG2b mAbs, the filtrated supernatant was directly loaded to a HiTrap Protein A HP Sepharose column (1 ml; GE Healthcare) pre-equilibrated with phosphate-buffered saline (PBS). After washing with PBS, IgG was eluted using IgG Elution buffer (Thermo Fisher Scientific) and the buffer pH was immediately neutralized using 1.5 M Tris–HCl (pH 8.8). IgG was concentrated up to ∼2 mg/ml in PBS using an Amicon Ultra filter (50 k-cut off; Millipore).

Purified IgG was conjugated with a fluorescent dye (Hayashi-Takanaka et al. 2014). Dried Alexa Fluor 488 5-SDP ester (1 mg; Invitrogen), Cy3 N-hydroxysuccinimide ester (for labeling 1 mg protein; GE Healthcare), and Cy5 N-hydroxysuccinimide ester (for labeling 1 mg protein; GE Healthcare) were dissolved into 100 μl dimethyl sulfoxide (DMSO; Wako) and stored at −20 °C. IgG (100 μg) was diluted into 100 mM NaHCO3 (pH 8.3) in 100 μl. After addition of a dye solution (1, 1.5, and 4 μl for Alexa488, Cy3, and Cy5, respectively), the mixture was incubated for 1 h at room temperature with gentle rotation. The sample was passed through a PD-mini G-25 desalting column (GE Healthcare), which was pre-equilibrated with PBS, to remove unconjugated dyes, and dye-conjugated IgG was concentrated up to ∼1 mg/ml using an Ultrafree 0.5 filter (10 k-cut off; Millipore). The IgG concentration and dye:protein ratio were calculated from the absorbance at 280 and 494, 552, or 650 nm, using the extinction coefficient of IgG and correction factor at 280 nm provided by the manufacturers. Fluorescent dye-labeled IgG samples that yielded dye:protein ratio ∼3–9 were used for live imaging.


hTERT-RPE1 (purchased from Clontech; September 22, 1999; the pseudodiploid karyotype occasionally checked with most recently in February 2015) and HeLa cells (obtained from Peter Cook; ref. Pombo et al. 1999) were grown in Dulbecco’s Modified Eagle’s medium (DMEM; Nacalai Tesque) supplemented with l-glutamine/penicillin/streptomycin (2 mM l-glutamine, 100 U/ml penicillin, 0.1 mg/ml streptomycin; Sigma-Aldrich) and 10 % fetal calf serum (FCS), as previously described (Hayashi-Takanaka et al. 2009).

For ChIP experiments, mouse embryonic stem cells (ZHBTc4 cell line) were obtained from RIKEN BRC on January 11, 2011 (Niwa et al. 2000). Cells were maintained on culture dishes coated with 0.1 % type-A gelatin (Sigma-Aldrich) in Glasgow Minimum Essential Medium (Sigma-Aldrich) supplemented with 10 % fetal bovine serum (Equitech-Bio, Kerrville, TX), 1× nonessential amino acids (Nacalai Tesque), 1 mM sodium pyruvate (Nacalai Tesque), 0.1 mM 2-mercaptoethanol (Nacalai Tesque), and 2000 U/ml Murine LIF.


Acetylated H4 tagged with NHis was produced by an in vitro translation system and purified as described (Mukai et al. 2011). Methylation mimic histones were purchased (Active Motif). HeLa histones were purified using the Histone Purification mini kit (Active Motif). H4 proteins (100 and 10 ng for Coomassiee-staining and immunoblotting, respectively) were separated in 15 % SDS-polyacrylamide gels (Wako), and either stained with Coomassiee Brilliant Blue (Wako) or transferred onto PVDF membranes (Pall) for immunoblotting using a semi-dry system (ATTO; 192 mM glycine, 100 mM Tris, and 5 % methanol; 70 min). PVDF membranes were washed with TBST (20 mM Tris–HCl (pH 8.0), 150 mM NaCl, and 0.05 % Tween 20), blocked with Blocking One-P (Nacalai Tesque) for 20 min, and incubated in mAbs (0.1–0.2 μg/ml) in TBST containing 10 % Blocking One-P for 1 h. After washing with TBST four times over 30 min, membranes were incubated in peroxidase-conjugated anti-mouse IgG (GE Healthcare; 1:1,000 in TBST) for 1 h, washed with TBST four times over 30 min, and soaked in chemiluminescent reagent (Perkin-Elmer). Chemiluminescent signals were captured using a LAS-3000 (Fuji film).

Expression vectors for lysine-to-alanine substitution mutants were constructed based on pBOS-H4-N-GFP (Kimura and Cook 2001) using a Quickchange kit (Stratagene) with specific primers containing single mutations. The resulting constructs were verified by nucleotide sequencing. HeLa cells grown on a 60-mm dish were transfected with plasmid DNA (2 μg) using Lipofectamine 2000 (Life Technologies; 10 μl) and further grown for a day. Cells were lysed using 2× SDS-gel loading buffer (0.5 ml) and boiling for 5 min, and 5 μl of lysate was separated in 10–20 % SDS-polyacrylamide gels (Wako) for immunoblotting.


Cells were fixed, permeabilized, and blocked as described previously (Kimura et al. 2006; Hayashi-Takanaka et al. 2009). To label replication foci, cells were incubated in 10 μM EdU for 7.5 min before fixation, and the signal was detected with Alexa Fluor 647 azide using a Click-iT EdU Imaging Kit (Life Technologies).

Cells were then incubated in labeled mAbs (0.2–1 μg/ml) with Hoechst 33342 (100 ng/ml in PBS) in PBS containing 10 % Blocking One-P (Nacalai Tesque) for 2 h at room temperature. After washing with PBS, coverslips were mounted in Prolong Gold (Life Technologies). Fluorescence images were collected using a confocal microscope (FV-1000; Olympus) operated by the built-in software (ver 2.1-4.1) with a PlanSApo 60× (NA = 1.35) or a PlanApoN SC 60× (NA = 1.4) objective lens (512 × 512 pixels; 12.5 μs/pixel; 4 line Kalman; 12-bit; pinhole 110 μm). Hoechst 33342, Alexa488, Cy3, and Cy5/Alexa647 signals were acquired by sequential scanning using laser lines at 405, 488, 543, and 633 nm, combined with emission dichroic mirror/barrier filter SDM490/BA430-470, SDM560/BA505-525, SDM640/BA560-620, and none/BA650IF, respectively, with a main dichroic mirror DM405/488/543/633.


Cells (ZHBTc4) were cross-linked in 0.5 % formaldehyde and suspended in 250 μl ChIP buffer (5 mM PIPES (pH 8.0), 200 mM KCl, 1 mM CaCl2, 1.5 mM MgCl2, 5 % sucrose, 0.5 % NP-40, and protease inhibitor cocktail; Nacalai Tesque). Samples were incubated for 15 min on ice, sonicated for 5 s three times, and digested with micrococcal nuclease (1 μl; New England Biolabs) and Ribonuclease A (1 μl; Nacalai Tesque) at 37 °C for 40 min. The digested samples were centrifuged at 15,000×g for 10 min. Supernatant containing 4–8 μg DNA was incubated with a mouse monoclonal antibody against H4K5ac, H4K8ac, H4K12ac, or H4K16ac, pre-bound to magnetic beads at 4 °C overnight with rotation. The immune complexes were washed twice with ChIP buffer and twice with TE buffer. After, cross-links were reversed by incubating in TE containing 1 % SDS at 65 °C overnight with proteinase K, and DNA was purified using a QIAquick PCR purification kit (Qiagen).

ChIP-seq data analysis

Sequenced reads of chromatin immunoprecipiation combined with deep sequencing (ChIP-seq) data were mapped onto the mouse genome (mm9) with Bowtie (version 0.12.8) using the parameter “-n 3 -m 2”. Estimation of normalized ChIP-seq signal intensities were calculated as follows. First, we counted mapped reads at 10,000 bp (Fig. 4a, b), 200 bp (Fig. 4c), or 100,000 bp (Fig. 4d) intervals (bins) on the genome. The counts were normalized as RPKM (reads per kilobase per million mapped reads) (Mortazavi et al. 2008). Net ChIP-seq signal intensities were obtained by subtracting the input RPKM in each bin (Accession number, DRA002438 for H4 acetylation data; and SRX185820 for H3K7ac data).

mRNA-seq and data analysis

Sequencing was performed using a Genome Analyzer IIx (Illumina). PolyA+ RNA was enriched from 100 ng of total RNA by two successive rounds of oligo(dT) selection. The polyA+ RNA was fragmented and first-strand cDNA was synthesized by random hexamer priming. Following second-strand cDNA synthesis, dsDNA was repaired using T4 DNA polymerase, Klenow enzyme, and T4 polynucleotide kinase (PNK) (New England Biolabs), followed by treatment with Klenow exo- to add an A base to the 3′ end. After ligation of the adaptor using TaKaRa Ligation Mix (TaKaRa), the adaptor-ligated DNA was amplified using PCR primers for 12 cycles, and the amplified library was isolated using the E-gel Electrophoresis System (Life Technologies). The samples were purified using a QIAquick MinElute Kit (Qiagen). Sequenced reads were mapped onto the mouse genome (mm9) using Tophat (version 2.0.10). Gene expression levels (FPKM; Fragments per kilobase of exon per million mapped sequence reads) were estimated by Cufflinks (version 2.1.1) using the parameters “-u -b (on cuffdiff)”. We classified genes into 11 groups based on the expression levels: Silent (FPKM = 0) and 10 groups with different expression levels separated at 10 percentile intervals (q0–10 %, q10–20 %, … and q90–100 % of genes from the lowest to the highest FPKM).

For a close look at ChIP-seq results, IGV_2.0.34 was used, as in Fig.4a, b.

Results and discussion

Generation of mAbs directed against modified histone H4

We generated mAbs directed against unmodified and specifically modified forms of histone H4, including acetylation at K5, K8, K12, and K16, and three levels of methylation at K20 (Table 1). The specificity was evaluated by ELISA and immunoblotting using synthetic peptides and recombinant proteins, respectively.

Pan-H4 antibody for immunoblotting

mAb CMA400 reacted with all peptides containing amino acid 11–29 of H4, regardless of modifications at K16 and K20, but not with those containing amino acid 1-19 (Fig. 1). This indicates that CMA400 recognizes the peptide sequence between amino acid 21 and 29. Consistently, immunoblotting showed that CMA400 reacts with all recombinant H4 proteins harboring acetylation at K5, K8, K12, K16, and K20, and methylation mimic modifications at K20 as well as H4 prepared from HeLa cells (Fig. 2a). Therefore, CMA400 appears to be useful as a pan-H4 mAb by immunoblotting. CMA400, however, failed to stain cell nuclei by immunofluorescence, possibly because the epitope region is masked due to the direct interaction between the H4 tail in a nucleosome and H2A in another nucleosome (Luger et al. 1997; Sinha and Shogren-Knaak 2010).
Fig. 2

Specificity of mAbs evaluated by immunoblotting. a mAb reactivity to recombinant and HeLa histone H4. NHis-tagged H4, NHis-tagged acetylated H4 (monoacetylated or tetraacetylated at K5/K8/K12/K16 [tetra-ac]), H4, methyl-mimic H4, and HeLa histones were separated in 15 % SDS-polyacrylamide gels and stained with Coomassiee Brilliant Blue (top panel) or probed with the indicated mAbs (lower panels). The positions of size standards are indicated on the left of the top panel. b mAb reactivity to GFP-H4 mutants. GFP-H4 (wild-type; wt) and lysine-to-alanine (KA) mutants were expressed in HeLa cells. Total protein was separated in 10–20 % SDS-polyacrylamide gel and probed with the indicated mAbs. The top panel with anti-H4K16ac mAb CMA416 shows a typical example. Both the endogenous H4 and GFP-tagged H4 and KA mutants except K16A are detected with CMA416. The positions of size standards are indicated on the right. The membrane was reprobed using GFP-specific antibody (second panel). Relevant parts are shown for other mAbs (lower panels). c CMA405 reactivity to recombinant histone H4. NHis-tagged H4 proteins were separated in 15 % SDS-polyacrylamide gels and stained with Coomassiee Brilliant Blue (top panel) or probed with CMA405 (bottom panel). The positions of size standards are indicated on the right

Acetylation-specific antibodies

ELISA and immunoblotting also revealed that modification-specific mAbs reacted exclusively with the corresponding peptides and recombinant H4 harboring the target modifications (Figs. 1 and 2). For example, CMA416 reacted with all peptides containing K16ac, regardless of the neighboring modifications such as K12ac, K20ac, and K20me1-3 by ELISA (Fig. 1). The specific binding of CMA416 to H4K16ac was confirmed by immunoblotting using recombinant H4; it highlighted H4 proteins harboring K16 acetylation and K5/K8/K12/K16 tetra-acetylation (Fig. 2a). In addition to the endogenous H4, exogenously expressed GFP-tagged H4 and mutant proteins, except H4K16A, were detected by CMA416 (Fig. 2b), indicating that H4K16 is required for CMA416 binding. From these data, it is concluded that CMA416 specifically binds to H4K16ac. Similarly, CMA408 and CMA412 specifically reacted with H4K8ac and H4K12ac, respectively, regardless of the neighboring modifications (Figs. 1 and 2).

Interestingly, CMA405 was specific to H4K5ac combined with unmodified K8, distinguishable from diacetylated H4 at K5 and K8 (Figs. 1 and 2). In ELISA, CMA405 reacted with monoacetylated peptide at K5 but not with diacetylated peptide at K5 and K8 (Fig. 1). Immunoblotting also showed that CMA405 exclusively reacted with K5-acetylated H4, but not with tetraacetylated H4 at K5/K8/K12/K16 (Fig. 2a), or H4K8A and H4K5A mutants (Fig. 2b). These results indicate that both acetylated K5 and unacetylated K8 are required for CMA405 binding. In Fig. 2b, CMA405 reacted more with H4K12A and H4K20A mutants, suggesting that CMA405 reactivity depends on these residues. To examine whether K12 acetylation influences the antibody recognition, we used recombinant histone H4 that harbors both K5 and K12 acetylation. As shown in Fig. 2c, CMA405 reacted equally with K5-acetylated and K5/K12-diacetylated H4, but not with K5/K8-diacetylated and K12-acetylated H4, indicating that CMA405 reactivity is not affected by K12 modification state. The differential reactivity to transiently expressed H4K12A mutant can be explained by the interplay between the modifications of different residues in living cells, as also observed in H4K12 acetylation (Fig. 2b; CMA412 blot) and in a previous study (Sasaki et al. 2009).

The unique characteristic of CMA405 appeared to selectively detect newly assembled H4, which is diacetylated at K5 and K12 (with unacetylated K8) over the hyperacetylated H4, which harbors acetylation on K5, K8, K12, and K16. Indeed, immnofluorescence revealed that only a fraction of cells were intensely stained with CMA405, and the intra-nuclear distribution resembled replication foci in S phase cells (Fig. 3a). To compare the distribution of CMA405 with replication foci, cells were pulse-labeled with ethynyl-deoxyuridine (EdU) for 7.5 min, fixed, and stained with Alexa Fluor 647-azide and CMA405. As shown in Fig 3a, CMA405 overlapped with Alexa647-labeled EdU, confirming that CMA405 detected newly assembled histone H4 that harbor K5 acetylation. CMA412 was also enriched in replication foci but still remained in euchromatin where the signal did not overlap with Hoechst (Fig. 3b). This is explained by the specificity of CMA412, which reacted with H4K12ac regardless of the neighboring K8 and K16 acetylation (Fig. 1). CMA412 could detect both newly assembled H4, which harbors acetylated K12 with unacetylated K8 and K16, and euchromatin-associated H4, which harbors acetylation at K8 and/or K16 together with K12. Immunofluorescence further revealed that CMA408 and CMA416 showed a euchromatin distribution, consistent with a role of H4K8 and H4K16 acetylation in chromatin decondensation and transcriptional activation (Fig. 3c, d).
Fig. 3

Localization of acetylation-specific mAbs. HeLa cells were fixed and immunolabeled with mAbs conjugated with Alexa Fluor 488 and Cy3. DNA was counterstained with Hoechst 33342. Shown are single confocal sections. a To label replication foci, cells were incubated with EdU for 7.5 min before fixation. EdU was detected with Alexa Fluor 647-azide before immunolabeling with H4K5-specific CMA405 conjugated with Alexa488. CMA405 overlaps with EdU (replication) foci in S phase cells. b H4K12ac-specific CMA412 (Alexa488) generally distributes in euchromatin and also enriched in CMA405 (Cy3) foci. c H4K8ac-specific CMA408 (Alexa488) is enriched in euchromatin. d H4K16ac-specific CMA416 (Alexa488) is enriched in euchromatin. Bars, 10 μm

ChIP-seq with acetylation-specific antibodies

To reveal the localization of acetylation at different lysines in H4, we performed ChIP-seq analysis using mouse embryonic stem cells with the specific antibodies. In a genome-wide view, H4K5ac was not enriched in specific loci, but was rather depleted from transcriptionally active regions, complementary to H4K8ac and H4K16ac (Fig. 4a, b). Aggregation plots confirmed that H4K5ac with unacetylated K8 (detected by CMA405) was excluded from transcription start sites (TSSs) of actively transcribed genes (Fig. 4c). This depletion in highly expressed genes probably reflects the absence of nucleosomes at TSSs and the presence of hyperacetylated H4, in which K5 and K8 are both acetylated, around TSSs. H4K12ac (detected by CMA412) was distributed similarly to H4K5ac, but slightly enriched in upstream and downstream regions from TSSs in expressed genes. This is consistent with the property of the antibody, which binds to H4K12ac regardless of acetylation at K8 and K16, and detects both replication-associated and euchromatin-associated H4K12ac. H4K8ac and H4K16ac (detected by CMA408 and CMA416, respectively) were enriched around the TSS of highly expressed genes like H3K27ac, but their distributions were broader and slightly more distant from the TSS than H3K27ac. Correlation analysis also indicated the high similarity between H4K8ac and H4K16ac (Fig. 4d). H3K27ac and H4K8ac, or H4K16ac, were also correlated, but not as much as the correlation between H4K8ac and H4K16ac, in good agreement with the aggregation plots (Fig. 4c). Little correlation was observed between H4K5ac and H4K8ac, consistent with their apparently distinct distributions by genome browser and aggregation plots.
Fig. 4

Localization of acetylated H4 in mouse ES cells by ChIP-seq. Genome-wide distribution of acetylated H4 was analyzed by ChIP-seq using CMA405, CMA408, CMA412, and CMA416. a Snapshot of the enrichment in whole chromosome 2. b Snapshot of the enrichment in chromosome 2 with a narrow view field. c Aggregation plots. Normalized peak counts of H4K5ac, H4K8ac, H4K12ac, H4K16ac, and H3K27ac signals surrounding the transcription start site (TSS) are indicated for 11 groups of genes which were divided based on their gene expression. d Correlation among histone H4K5, K8, K16, and H3K27ac modifications. H4K8ac, K16, and H3K27ac have positive correlation but not with H4K5ac

Methylation-specific antibodies

Among the H4K20 methylation-specific mAbs, H4K20me1- and H4K20me3-specific mAbs (CMA421 and CMA423) reacted with their targets in the presence of K16ac, and H4K20me2-specific CMA422 hardly reacted with the peptide containing K16ac with K20me2 (Fig. 1). Immunoblotting showed these antibodies reacted specifically with the corresponding methylation mimic H4 proteins (Fig. 2). The specificity of each mAb to the target modification was also confirmed by their characteristic immunofluorescence patterns. hTERT-RPE1 cells stained with CMA421 showed heterogeneity in signal intensity (Fig. 5a), in good agreement with the previous report showing that H4K20me1 level increases during G2 to M phase (Rice et al. 2002; Pesavento et al. 2008). Indeed, interphase cells positive in H3S10 phosphorylation, which begins to appear in late S through G2, were often heavily stained with CMA421 (Fig. 5a). Thus, double staining cells with CMA405 (to detect S phase) and CMA421 (to detect G2 phase) was able to identify the cell cycle stage of individual cells (Fig. 5a). Furthermore, inactive X chromosomes were highlighted with CMA421 in hTERT-RPE1 cells (Fig. 5b), as shown previously. CMA422 and CMA423 exhibited DAPI-like and heterochromatin patterns, respectively, again consistent with previous observations (Fig. 5c, d).
Fig. 5

Localization of methylation-specific mAbs. hTERT-RPE1 (a, b) and HeLa (c, d) cells were fixed, immunolabeled with the indicated mAbs conjugated with Alexa Fluor 488, Cy3, or Cy5. DNA was counterstained with Hoechst 33342. Shown are single confocal sections. a hTERT-RPE1 cells were stained with H4K20me1-specific CMA421 (Alexa488), H3S10ph-specific CMA313 (Cy3), and H4K5ac-specific CMA405 (Cy5). The cell cycle points, judged from the staining patterns of H3S10ph and H4K5ac, are indicated. CMA421 is enriched in G2 cells (H3S10ph-positive; H4K5ac-negative) than cells in G1 (H3S10ph-negative; H4K5ac-negative) and S (H3S10-negative; H4K5ac-positive). Under the condition to detect H3S10ph signals in G2 cells, those on mitotic chromosomes are saturated (asterisk) due to massive phosphorylation during M phase. b hTERT-RPE1 cells were stained with H4K20me1-specific CMA421 (Alexa488) and H3K27me3-specific CMA323 (Cy3). CMA421 is concentrated on inactive X chromosome indicated by H3K27me3. c HeLa cells were stained with H4K20me2-specific CMA422 (Alexa488). CMA422 is distributed similarly to Hoechst 33342. d HeLa cells were stained with H4K20me3-specific CMA423 (Alexa488). CMA423 is concentrated on Hoechst-dense heterochromatin. Bars, 10 μm


We showed that mouse mAbs directed against histone H4 and its modifications are highly specific to the targets and are applicable to immunoblotting, immunofluorescence, and ChIP-seq. While histone modification-specific antibodies are now widely available from different vendors, most of them are rabbit polyclonals and therefore associated with lot-to-lot variation of the specificity and affinity. Furthermore, a systematic study revealed that 20–25 % of histone modification-specific antibodies were disqualified for ChIP-seq application (Egelhofer et al. 2011). As the property of mAb remains constant over time, new H4-specific mAbs described here will be useful for future epigenetics and epigenome studies. In fact, some of these antibodies have already proven useful for immunofluorescence with mouse tissue sections (Eberhart et al 2012, 2013), histochemistry with human patient specimen (Yokoyama et al 2014; Shinchi et al 2015), and ChIP-seq using chicken DT40 cells (Hori et al 2014).

Among the mAbs described here, H4K5ac-specific mAb CMA405 has a unique specificity. It recognizes acetylated K5 with unacetylated K8. This combination is typically found in newly assembled H4, which is diacetylated at K5 and K12, and CMA405 indeed highlights structures that resemble DNA replication foci in S phase cells. Thus, CMA405 will be particularly useful for detecting newly assembled chromatin and also for highlighting cells in S phase by microscopy and flow cytometry.



We thank Hitoshi Niwa (RIKEN CDB) for ZHBTc4 mouse embryonic stem cells and Timothy Stasevich (Colorado State University) for proofreading the manuscript. This work was supported by the New Energy and Industrial Technology Development Organization of Japan, grants-in-aid from the Japan Society for the Promotion of Science (25116005 and 26291071), and the Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science and Technology of Japan. Y.H.-T. was supported by a Japan Society for the Promotion of Science fellowship and by the Naito Foundation.

Conflict of interest

N.N. is a founder of MAB Institute Inc. The other authors declare that they have no conflict of interests.


  1. Bannister AJ, Kouzarides T (2011) Regulation of chromatin by histone modifications. Cell Res 21:381–395. doi: 10.1038/cr.2011.22 PubMedCentralCrossRefPubMedGoogle Scholar
  2. Barman HK, Takami Y, Ono T, Nishijima H, Sanematsu F, Shibahara K, Nakayama T (2006) Histone acetyltransferase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells. Biochem Biophys Res Commun 345:1547–1557. doi: 10.1016/j.bbrc.2006.05.079 CrossRefPubMedGoogle Scholar
  3. Beck DB, Oda H, Shen SS, Reinberg D (2012) PR-Set7 and H4K20me1: at the crossroads of genome integrity, cell cycle, chromosome condensation, and transcription. Genes Dev 26:325–337. doi: 10.1101/gad.177444.111 PubMedCentralCrossRefPubMedGoogle Scholar
  4. Chandra T, Kirschner K, Thuret JY, Pope BD, Ryba T, Newman S, Ahmed K, Samarajiwa SA, Salama R, Carroll T, Stark R, Janky R, Narita M, Xue L, Chicas A, Nũnez S, Janknecht R, Hayashi-Takanaka Y, Wilson MD, Marshall A, Odom DT, Babu MM, Bazett-Jones DP, Tavaré S, Edwards PA, Lowe SW, Kimura H, Gilbert DM, Narita M (2012) Independence of repressive histone marks and chromatin compaction during senescent heterochromatic layer formation. Mol Cell 47:203–214. doi: 10.1016/j.molcel.2012.06.010 PubMedCentralCrossRefPubMedGoogle Scholar
  5. Chang L, Loranger SS, Mizzen C, Ernst SG, Allis CD, Annunziato AT (1997) Histones in transit: cytosolic histone complexes and diacetylation of H4 during nucleosome assembly in human cells. Biochemistry 36:469–480. doi: 10.1021/bi962069i CrossRefPubMedGoogle Scholar
  6. Clayton AL, Hazzalin CA, Mahadevan LC (2006) Enhanced histone acetylation and transcription: a dynamic perspective. Mol Cell 23:289–296. doi: 10.1016/j.molcel.2006.06.017 CrossRefPubMedGoogle Scholar
  7. Dang W, Steffen KK, Perry R, Dorsey JA, Johnson FB, Shilatifard A, Kaeberlein M, Kennedy BK, Berger SL (2009) Histone H4 lysine 16 acetylation regulates cellular lifespan. Nature 459:802–807. doi: 10.1038/nature08085 PubMedCentralCrossRefPubMedGoogle Scholar
  8. Dion MF, Altschuler SJ, Wu LF, Rando OJ (2005) Genomic characterization reveals a simple histone H4 acetylation code. Proc Natl Acad Sci U S A 102:5501–5506. doi: 10.1073/pnas.0500136102 PubMedCentralCrossRefPubMedGoogle Scholar
  9. Eberhart A, Kimura H, Leonhardt H, Joffe B, Solovei I (2012) Reliable detection of epigenetic histone marks and nuclear proteins in tissue cryosections. Chromosom Res 20:849–858. doi: 10.1007/s10577-012-9318-8 CrossRefGoogle Scholar
  10. Eberhart A, Feodorova Y, Song C, Wanner G, Kiseleva E, Furukawa T, Kimura H, Schotta G, Leonhardt H, Joffe B, Solovei I (2013) Epigenetics of eu- and heterochromatin in inverted and conventional nuclei from mouse retina. Chromosom Res 21:535–554. doi: 10.1007/s10577-013-9375-7 CrossRefGoogle Scholar
  11. Egelhofer TA, Minoda A, Klugman S, Lee K, Kolasinska-Zwierz P, Alekseyenko AA, Cheung MS, Day DS, Gadel S, Gorchakov AA, Gu T, Kharchenko PV, Kuan S, Latorre I, Linder-Basso D, Luu Y, Ngo Q, Perry M, Rechtsteiner A, Riddle NC, Schwartz YB, Shanower GA, Vielle A, Ahringer J, Elgin SC, Kuroda MI, Pirrotta V, Ren B, Strome S, Park PJ, Karpen GH, Hawkins RD, Lieb JD (2011) An assessment of histone-modification antibody quality. Nat Struct Mol Biol 18:91–93. doi: 10.1038/nsmb.1972 PubMedCentralCrossRefPubMedGoogle Scholar
  12. Ejlassi-Lassallette A, Mocquard E, Arnaud MC, Thiriet C (2011) H4 replication-dependent diacetylation and Hat1 promote S-phase chromatin assembly in vivo. Mol Biol Cell 22:245–255. doi: 10.1091/mbc.E10-07-0633 PubMedCentralCrossRefPubMedGoogle Scholar
  13. Ellis L, Atadja PW, Johnstone RW (2009) Epigenetics in cancer: targeting chromatin modifications. Mol Cancer Ther 8:1409–1420. doi: 10.1158/1535-7163.MCT-08-0860 CrossRefPubMedGoogle Scholar
  14. Fraga MF, Ballestar E, Villar-Garea A, Boix-Chornet M, Espada J, Schotta G, Bonaldi T, Haydon C, Ropero S, Petrie K, Iyer NG, Pérez-Rosado A, Calvo E, Lopez JA, Cano A, Calasanz MJ, Colomer D, Piris MA, Ahn N, Imhof A, Caldas C, Jenuwein T, Esteller M (2005) Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer. Nat Genet 37:391–400. doi: 10.1038/ng1531 CrossRefPubMedGoogle Scholar
  15. Greer EL, Shi Y (2012) Histone methylation: a dynamic mark in health, disease and inheritance. Nat Rev Genet 13:343–357. doi: 10.1038/nrg3173 PubMedCentralCrossRefPubMedGoogle Scholar
  16. Greeson NT, Sengupta R, Arida AR, Jenuwein T, Sanders SL (2008) Di-methyl H4 lysine 20 targets the checkpoint protein Crb2 to sites of DNA damage. J Biol Chem 283:33168–33174. doi: 10.1074/jbc.M806857200 PubMedCentralCrossRefPubMedGoogle Scholar
  17. Hayashi-Takanaka Y, Yamagata K, Nozaki N, Kimura H (2009) Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase. J Cell Biol 187:781–790. doi: 10.1083/jcb.200904137 PubMedCentralCrossRefPubMedGoogle Scholar
  18. Hayashi-Takanaka Y, Yamagata K, Wakayama T, Stasevich TJ, Kainuma T, Tsurimoto T, Tachibana M, Shinkai Y, Kurumizaka H, Nozaki N, Kimura H (2011) Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling. Nucleic Acids Res 39:6475–6488. doi: 10.1093/nar/gkr343 PubMedCentralCrossRefPubMedGoogle Scholar
  19. Hayashi-Takanaka Y, Stasevich TJ, Kurumizaka H, Nozaki N, Kimura H (2014) Evaluation of chemical fluorescent dyes as a protein conjugation partner for live cell imaging. PLoS One 9:e106271. doi: 10.1371/journal.pone.0106271 PubMedCentralCrossRefPubMedGoogle Scholar
  20. Heintzman ND, Stuart RK, Hon G, Fu Y, Ching CW, Hawkins RD, Barrera LO, Van Calcar S, Qu C, Ching KA, Wang W, Weng Z, Green RD, Crawford GE, Ren B (2007) Distinct and predictive chromatin signatures of transcriptional promoters and enhancers in the human genome. Nat Genet 39:311–318. doi: 10.1038/ng1966 CrossRefPubMedGoogle Scholar
  21. Hori T, Shang WH, Toyoda A, Misu S, Monma N, Ikeo K, Molina O, Vargiu G, Fujiyama A, Kimura H, Earnshaw WC, Fukagawa T (2014) Histone H4 Lys 20 monomethylation of the CENP-A nucleosome is essential for kinetochore assembly. Dev Cell 29:740–749. doi: 10.1016/j.devcel.2014.05.001 PubMedCentralCrossRefPubMedGoogle Scholar
  22. Johnson CA, O'Neill LP, Mitchell A, Turner BM (1998) Distinctive patterns of histone H4 acetylation are associated with defined sequence elements within both heterochromatic and euchromatic regions of the human genome. Nucleic Acids Res 26:994–1001. doi: 10.1093/nar/26.4.994 PubMedCentralCrossRefPubMedGoogle Scholar
  23. Jørgensen S, Schotta G, Sørensen CS (2013) Histone H4 lysine 20 methylation: key player in epigenetic regulation of genomic integrity. Nucleic Acids Res 41:2797–2806. doi: 10.1093/nar/gkt012 PubMedCentralCrossRefPubMedGoogle Scholar
  24. Kapoor-Vazirani P, Vertino PM (2014) A dual role of PR-SET7-mediated H4K20me1 in the local regulation of RNA polymerase II promoter-proximal pausing. J Biol Chem 289:7425–7437. doi: 10.1074/jbc.M113.520783 PubMedCentralCrossRefPubMedGoogle Scholar
  25. Kimura H (2013) Histone modifications for human epigenome analysis. J Hum Genet 58:439–445. doi: 10.1038/jhg.2013.66 CrossRefPubMedGoogle Scholar
  26. Kimura H, Cook PR (2001) Kinetics of core histones in living human cells: little exchange of H3 and H4 and some rapid exchange of H2B. J Cell Biol 153:1341–1353. doi: 10.1083/jcb.153.7.1341 PubMedCentralCrossRefPubMedGoogle Scholar
  27. Kimura H, Nozaki N, Saijo M, Kikuchi A, Ui M, Enomoto T (1994) Identification of the nature of modification that causes the shift of DNA topoisomerase II beta to apparent higher molecular weight forms in the M phase. J Biol Chem 269:21439–21445. doi: 10.1074/jbc.271.35.21439 Google Scholar
  28. Kimura H, Takizawa N, Allemand E, Hori T, Iborra FJ, Nozaki N, Muraki M, Hagiwara M, Krainer AR, Fukagawa T, Okawa K (2006) A novel histone-exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A-H2B. J Cell Biol 175:389–400. doi: 10.1083/jcb.200608001 PubMedCentralCrossRefPubMedGoogle Scholar
  29. Kimura H, Hayashi-Takanaka Y, Goto Y, Takizawa N, Nozaki N (2008) The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies. Cell Struct Funct 33:61–73. doi: 10.1247/csf.07035 CrossRefPubMedGoogle Scholar
  30. Krishnan V, Chow MZ, Wang Z, Zhang L, Liu B, Liu X, Zhou Z (2011) Histone H4 lysine 16 hypoacetylation is associated with defective DNA repair and premature senescence in Zmpste24-deficient mice. Proc Natl Acad Sci U S A 108:12325–12330. doi: 10.1073/pnas.1102789108 PubMedCentralCrossRefPubMedGoogle Scholar
  31. Lang D, Schümann M, Gelato K, Fischle W, Schwarzer D, Krause E (2013) Probing the acetylation code of histone H4. Proteomics 13:2989–2997. doi: 10.1002/pmic.201200568 PubMedGoogle Scholar
  32. Li X, Corsa CA, Pan PW, Wu L, Ferguson D, Yu X, Min J, Dou Y (2010) MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1. Mol Cell Biol 30:5335–5347. doi: 10.1128/MCB.00350-10 PubMedCentralCrossRefPubMedGoogle Scholar
  33. Luger K, Mäder AW, Richmond RK, Sargent DF, Richmond TJ (1997) Crystal structure of the nucleosome core particle at 2.8 A resolution. Nature 389:251–260. doi: 10.1038/38444 CrossRefPubMedGoogle Scholar
  34. Ma XJ, Wu J, Altheim BA, Schultz MC, Grunstein M (1998) Deposition-related sites K5/K12 in histone H4 are not required for nucleosome deposition in yeast. Proc Natl Acad Sci U S A 95:6693–6698PubMedCentralCrossRefPubMedGoogle Scholar
  35. Martin C, Zhang Y (2005) The diverse functions of histone lysine methylation. Nat Rev Mol Cell Biol 6:838–849. doi: 10.1038/nrm1761 CrossRefPubMedGoogle Scholar
  36. Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-seq. Nat Methods 5:621–628. doi: 10.1038/nmeth.1226 CrossRefPubMedGoogle Scholar
  37. Mukai T, Yanagisawa T, Ohtake K, Wakamori M, Adachi J, Hino N, Sato A, Kobayashi T, Hayashi A, Shirouzu M, Umehara T, Yokoyama S, Sakamoto K (2011) Genetic-code evolution for protein synthesis with non-natural amino acids. Biochem Biophys Res Commun 411:757–761. doi: 10.1016/j.bbrc.2011.07.020 CrossRefPubMedGoogle Scholar
  38. Niwa H, Miyazaki J, Smith AG (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 24:372–376. doi: 10.1016/j.bbrc.2011.07.020 CrossRefPubMedGoogle Scholar
  39. Pesavento JJ, Yang H, Kelleher NL, Mizzen CA (2008) Certain and progressive methylation of histone H4 at lysine 20 during the cell cycle. Mol Cell Biol 28:468–486. doi: 10.1128/MCB.01517-07 PubMedCentralCrossRefPubMedGoogle Scholar
  40. Peters AH, Kubicek S, Mechtler K, O'Sullivan RJ, Derijck AA, Perez-Burgos L, Kohlmaier A, Opravil S, Tachibana M, Shinkai Y, Martens JH, Jenuwein T (2003) Partitioning and plasticity of repressive histone methylation states in mammalian chromatin. Mol Cell 12:1577–1589. doi: 10.1016/S1097-2765(03)00477-5 CrossRefPubMedGoogle Scholar
  41. Pombo A, Jackson DA, Hollinshead M, Wang Z, Roeder RG, Cook PR (1999) Regional specialization in human nuclei: visualization of discrete sites of transcription by RNA polymerase III. EMBO J 18:2241–2253PubMedCentralCrossRefPubMedGoogle Scholar
  42. Rice JC, Nishioka K, Sarma K, Steward R, Reinberg D, Allis CD (2002) Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes. Genes Dev 16:2225–2230. doi: 10.1101/gad.1014902 PubMedCentralCrossRefPubMedGoogle Scholar
  43. Rodriguez-Paredes M, Esteller M (2011) Cancer epigenetics reaches mainstream oncology. Nat Med 17:330–339. doi: 10.1038/nm.2305 CrossRefPubMedGoogle Scholar
  44. Rothbart SB, Lin S, Britton LM, Krajewski K, Keogh MC, Garcia BA, Strahl BD (2012) Poly-acetylated chromatin signatures are preferred epitopes for site-specific histone H4 acetyl antibodies. Sci Rep 2:489. doi: 10.1038/srep00489 PubMedCentralCrossRefPubMedGoogle Scholar
  45. Sasaki K, Ito T, Nishino N, Khochbin S, Yoshida M (2009) Real-time imaging of histone H4 hyperacetylation in living cells. Proc Natl Acad Sci U S A 106:16257–16262. doi: 10.1073/pnas.0902150106 PubMedCentralCrossRefPubMedGoogle Scholar
  46. Schotta G, Lachner M, Sarmam K, Ebertm A, Sengupta R, Reuter G, Reinberg D, Jenuwein T (2004) A silenced pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin. Genes Dev 18:1251–1262. doi: 10.1101/gad.300704 PubMedCentralCrossRefPubMedGoogle Scholar
  47. Schotta G, Sengupta R, Kubicek S, Malin S, Kauer M, Callén E, Celeste A, Pagani M, Opravil S, De La Rosa-Velazquez IA, Espejo A, Bedford MT, Nussenzweig A, Busslinger M, Jenuwein T (2008) A chromatin-wide transition to H4K20 mono-methylation impairs genome integrity and programmed DNA rearrangements in the mouse. Genes Dev 22:2048–2061. doi: 10.1101/gad.476008 PubMedCentralCrossRefPubMedGoogle Scholar
  48. Shahbazian MD, Grunstein M (2007) Functions of site-specific histone acetylation and deacetylation. Annu Rev Biochem 76:75–100CrossRefPubMedGoogle Scholar
  49. Sharma GG, So S, Gupta A, Kumar R, Cayrou C, Avvakumov N, Bhadra U, Pandita RK, Porteus MH, Chen DJ, Cote J, Pandita TK (2010) MOF and histone H4 acetylation at lysine 16 are critical for DNA damage response and double-strand break repair. Mol Cell Biol 30:3582–3595. doi: 10.1128/MCB.01476-09 PubMedCentralCrossRefPubMedGoogle Scholar
  50. Shilatifard A (2008) Molecular implementation and physiological roles for histone H3 lysine 4 (H3K4) methylation. Curr Opin Cell Biol 20:341–348. doi: 10.1016/ PubMedCentralCrossRefPubMedGoogle Scholar
  51. Shinchi Y, Hieda M, Nishioka Y, Matsumoto A, Yokoyama Y, Kimura H, Matsuura S, Matsuura N (2015) SUV420H2 suppresses breast cancer cell invasion through downregulation of the SH2 domain-containing focal adhesion protein tensin-3. Exp Cell Res 334:90–99. doi: 10.1016/j.yexcr.2015.03.010 CrossRefPubMedGoogle Scholar
  52. Sims JK, Houston SI, Magazinnik T, Rice JC (2006) A trans-tail histone code defined by monomethylated H4 Lys-20 and H3 Lys-9 demarcates distinct regions of silent chromatin. J Biol Chem 281:12760–12766. doi: 10.1074/jbc.M513462200 CrossRefPubMedGoogle Scholar
  53. Sinha D, Shogren-Knaak MA (2010) Role of direct interactions between the histone H4 Tail and the H2A core in long range nucleosome contacts. J Biol Chem 285:16572–16581. doi: 10.1074/jbc.M109.091298 PubMedCentralCrossRefPubMedGoogle Scholar
  54. Sobel RE, Cook RG, Perry CA, Annunziato AT, Allis CD (1995) Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4. Proc Natl Acad Sci U S A 92:1237–1241PubMedCentralCrossRefPubMedGoogle Scholar
  55. Stasevich TJ, Hayashi-Takanaka Y, Sato Y, Maehara K, Ohkawa Y, Sakata-Sogawa K, Tokunaga M, Nagase T, Nozaki N, McNally JG, Kimura H (2014) Regulation of RNA polymerase II activation by histone acetylation in single living cells. Nature 516:272–275. doi: 10.1038/nature13714 CrossRefPubMedGoogle Scholar
  56. Taddei A, Roche D, Sibarita JB, Turner BM, Almouzni G (1999) Duplication and maintenance of heterochromatin domains. J Cell Biol 147:1153–1166. doi: 10.1083/jcb.147.6.1153 PubMedCentralCrossRefPubMedGoogle Scholar
  57. Taylor GC, Eskeland R, Hekimoglu-Balkan B, Pradeepa MM, Bickmore WA (2013) H4K16 acetylation marks active genes and enhancers of embryonic stem cells, but does not alter chromatin compaction. Genome Res 23:2053–2065. doi: 10.1101/gr.155028.113 PubMedCentralCrossRefPubMedGoogle Scholar
  58. The ENCODE Project Consortium (2012) An integrated encyclopedia of DNA elements in the human genome. Nature 489:57–74. doi: 10.1038/nature11247 PubMedCentralCrossRefGoogle Scholar
  59. Turner BM (1991) Histone acetylation and control of gene expression. J Cell Sci 99:13–20PubMedGoogle Scholar
  60. Turner BM, O'Neill LP, Allan IM (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies. FEBS Lett 253:141–145CrossRefPubMedGoogle Scholar
  61. Turner BM, Birley AJ, Lavender J (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei. Cell 69:375–384. doi: 10.1016/0092-8674(92)90417-B CrossRefPubMedGoogle Scholar
  62. Wang Z, Zang C, Rosenfeld JA, Schones DE, Barski A, Cuddapah S, Cui K, Roh TY, Peng W, Zhang MQ, Zhao K (2008) Combinatorial patterns of histone acetylations and methylations in the human genome. Nat Genet 40:897–903. doi: 10.1038/ng.154 PubMedCentralCrossRefPubMedGoogle Scholar
  63. Wu S, Rice JC (2011) A new regulator of the cell cycle: the PR-Set7 histone methyltransferase. Cell Cycle 10:68–72. doi: 10.4161/cc.10.1.14363 PubMedCentralCrossRefPubMedGoogle Scholar
  64. Yokoyama Y, Matsumoto A, Hieda M, Shinchi Y, Ogihara E, Hamada M, Nishioka Y, Kimura H, Yoshidome K, Tsujimoto M, Matsuura N (2014) Loss of histone H4K20 trimethylation predicts poor prognosis in breast cancer and is associated with invasive activity. Breast Cancer Res 16:R66. doi: 10.1186/bcr3681 PubMedCentralCrossRefPubMedGoogle Scholar
  65. Zheng Y, Thomas PM, Kelleher NL (2013) Measurement of acetylation turnover at distinct lysines in human histones identifies long-lived acetylation sites. Nat Commun 4:2203. doi: 10.1038/ncomms3203 PubMedCentralPubMedGoogle Scholar

Copyright information

© The Author(s) 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Yoko Hayashi-Takanaka
    • 1
    • 2
    • 3
  • Kazumitsu Maehara
    • 4
  • Akihito Harada
    • 4
  • Takashi Umehara
    • 5
  • Shigeyuki Yokoyama
    • 6
  • Chikashi Obuse
    • 7
  • Yasuyuki Ohkawa
    • 3
    • 4
  • Naohito Nozaki
    • 8
  • Hiroshi Kimura
    • 1
    • 2
    • 3
    Email author
  1. 1.Graduate School of Bioscience and BiotechnologyTokyo Institute of TechnologyYokohamaJapan
  2. 2.Graduate School of Frontier BiosciencesOsaka UniversitySuitaJapan
  3. 3.CRESTJSTKawaguchiJapan
  4. 4.Department of Advanced Medical Initiatives, Faculty of MedicineKyushu UniversityFukuokaJapan
  5. 5.RIKEN Center for Life Science TechnologiesYokohamaJapan
  6. 6.RIKEN Structural Biology LaboratoryYokohamaJapan
  7. 7.School of Life SciencesHokkaido UniversitySapporoJapan
  8. 8.MAB Institute IncSapporoJapan

Personalised recommendations