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Chromosome Research

, Volume 22, Issue 4, pp 495–503 | Cite as

Distribution of TTAGG-specific telomerase activity in insects

  • Michala Korandová
  • Tomáš Krůček
  • Kristýna Vrbová
  • Radmila Čapková FrydrychováEmail author
Article

Abstract

In most eukaryotes, telomeres consist of tandem arrays of a short repetitive DNA sequence. Insect telomeres are generally constituted by a (TTAGG)n repeat motif. Usually, telomeres are maintained by telomerase, a specialized reverse transcriptase that adds this sequence to chromosome ends. We examined telomerase activity in 15 species across Insecta. Telomerase activity was revealed in Isoptera, Blattaria, Lepidoptera, Hymenoptera, Trichoptera, Coleoptera, and Sternorrhyncha. In contrast, we were not able to detect telomerase activity in Orthoptera, Zygentoma, and Phasmida. Because we found telomerase activity in phylogenetically distant species, we conclude that a distribution pattern of (TTAGG)n sequence in Insecta is generally consistent with that of telomerase activity. Thus, the TTAGG-telomerase system is functional across the Insecta. Using real-time quantitative telomeric repeat amplification protocol (RTQ-TRAP) system, we quantified telomerase activity in different developmental stages and different tissues of a cockroach, Periplaneta americana. We show that telomerase is upregulated in young instars and gradually declines during development. In adults, it is most active in testes and ovaries. Thus, the telomerase activity of hemimetabolous insects seems to be associated with cell proliferation and organismal development.

Keywords

Insects telomere telomerase 

Abbreviations

TERT

Telomerase reverse transcriptase (TERT)

TRAP

Telomeric repeat amplification protocol

RTQ-TRAP

Real-time quantitative TRAP

Notes

Acknowledgments

We are grateful to James Mason for critical review of the manuscript, Aleš Bezděk, Jan Šobotník, Petr Doležal, and David Boukal, who provided us with live materials. This work was supported by the Grant No. 14-07172S from the Grant Agency of the Czech Republic. We acknowledge the use of research infrastructure that has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement No. 316304. Additional support was provided from Grant 052/2013/P of the Grant Agency of the University of South Bohemia.

Ethical standards

The authors declare that all experiments performed in this study comply with the current laws of the Czech Republic. All institutional and national guidelines for the care and use of laboratory animals were followed.

Conflict of interest

Michala Korandová, Tomáš Krůček, Kristýna Vrbová, and Radmila Čapková Frydrychová declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Michala Korandová
    • 1
  • Tomáš Krůček
    • 1
    • 2
  • Kristýna Vrbová
    • 1
  • Radmila Čapková Frydrychová
    • 2
    Email author
  1. 1.Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic
  2. 2.Biology Centre AS CRInstitute of EntomologyČeské BudějoviceCzech Republic

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