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Chromosome Research

, Volume 21, Issue 4, pp 419–432 | Cite as

Molecular cytogenetic characterization of a new wheat–rye 4R chromosome translocation line resistant to powdery mildew

  • Diaoguo AnEmail author
  • Qi Zheng
  • Yilin Zhou
  • Pengtao Ma
  • Zhenling Lv
  • Lihui Li
  • Bin Li
  • Qiaoling Luo
  • Hongxing Xu
  • Yunfeng Xu
Article

Abstract

Rye is an important and valuable gene resource for wheat improvement. However, due to extensive growing of cultivars with disease resistance genes from short arm of rye chromosome 1R and coevolution of pathogen virulence and host resistance, these cultivars successively lost resistance to pathogens. Identification and deployment of new resistance gene sources in rye are, therefore, of especial importance and urgency. A new wheat–rye line, designated as WR41-1, was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White. It was proved to be a new wheat–rye T4BL·4RL and T7AS·4RS translocation line using sequential genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), and expressed sequence tag-simple sequence repeat (EST-SSR) marker analysis. WR41-1 showed high levels of resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) pathogens prevalent in China at the adult growth stage and 13 of 23 Bgt isolates tested at the seedling stage. According to its resistant pattern to 23 different Bgt isolates, WR41-1 may possess new gene(s) for resistance to powdery mildew, which differed from previously identified and known powdery mildew genes from rye (Pm7, Pm8, Pm17, and Pm20). In addition, WR41-1 was cytologically stable, had a desirable fertility, and is expected to be useful in wheat improvement.

Keywords

Secale cereale chromosome translocation Blumeria graminis GISH and FISH EST-based marker wheat 

Abbreviations

Bgt

Blumeria graminis f. sp. tritici

CS

Chinese Spring wheat

DAPI

4,6-Diamidino-2-phenylindole

EST-SSR

Expressed sequence tag-simple sequence repeat

FISH

Fluorescence in situ hybridization

FITC

Fluorescence isothiocyanate

GISH

Genomic in situ hybridization

IT

Infection type

mc-FISH

Multicolor FISH

PCR

Polymerase chain reaction

TKW

Thousand-kernel weight

Notes

Acknowledgments

This research was supported by the National High-Tech Research and Development Program of China No. 2011AA1001, the National Natural Science Foundation of China No. 31171550, and the National Scientific and Technological Supporting Program of China No. 2013BAD01B02.

Conflict of interest

The authors (Diaoguo An, Qi Zheng, Yilin Zhou, Pengtao Ma, Zhenling Lv, Lihui Li, Bin Li, Qiaoling Luo, Hongxing Xu and Yunfeng Xu) declare that our experiments comply with the current laws of China and we have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Diaoguo An
    • 1
    Email author
  • Qi Zheng
    • 2
  • Yilin Zhou
    • 4
  • Pengtao Ma
    • 1
  • Zhenling Lv
    • 2
  • Lihui Li
    • 3
  • Bin Li
    • 2
  • Qiaoling Luo
    • 1
  • Hongxing Xu
    • 1
  • Yunfeng Xu
    • 1
  1. 1.Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology (IGDB), Chinese Academy of Sciences (CAS)ShijiazhuangChina
  2. 2.The State Key Laboratory of Plant Cell and Chromosome Engineering, IGDB, CASBeijingChina
  3. 3.The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)BeijingChina
  4. 4.The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, CAASBeijingChina

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