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Chromosome Research

, Volume 21, Issue 4, pp 383–392 | Cite as

A phylogenetic analysis using multidirectional chromosome painting of three species (Uroderma magnirostrum, U. bilobatum and Artibeus obscurus) of subfamily Stenodermatinae (Chiroptera-Phyllostomidae)

  • J. C. PieczarkaEmail author
  • A. J. B. Gomes
  • C. Y. Nagamachi
  • D. C. C. Rocha
  • J. D. Rissino
  • P. C. M. O’Brien
  • F. Yang
  • M. A. Ferguson-Smith
Article

Abstract

The species of genera Uroderma and Artibeus are medium-sized bats belonging to the family Phyllostomidae and subfamily Stenodermatinae (Mammalia, Chiroptera) from South America. They have a wide distribution in the Neotropical region, with two currently recognized species in Uroderma and approximately 20 species in Artibeus. These two genera have different rates of chromosome evolution, with Artibeus probably having retained the ancestral karyotype for the subfamily. We used whole chromosome paint probe sets from Carollia brevicauda and Phyllostomus hastatus on Uroderma magnirostrum, Uroderma bilobatum, and Artibeus obscurus. With the aim of testing the previous phylogenies of these bats using cytogenetics, we compared these results with published painting maps on Phyllostomidae. The genome-wide comparative maps based on chromosome painting and chromosome banding reveal the chromosome forms that characterize each taxonomic level within the Phyllostomidae and show the chromosome evolution of this family. Based on this, we are able to suggest an ancestral karyotype for Phyllostomidae. Our cladistic analysis is an independent confirmation using multidirectional chromosome painting of the previous Phyllostomidae phylogenies.

Key words

Uroderma Artibeus Chromosomal evolution Biodiversity FISH cladistic 

Abbreviations

ACI

Artibeus cinereus

AOB

Artibeus obscurus

CBR

Carollia brevicauda

DEC

Diphylla ecaudata

DRO

Desmodus rotundus

DYO

Diaemus youngi

FISH

Fluorescence in situ hybridization

PAUP

Phylogenetic analysis using parsimony

PHA

Phyllostomus hastatus

SSC

Saline Sodium Citrate

TBR

Tree bisection reconnection branch swapping

UBI

Uroderma bilobatum

UMA

Uroderma magnirostrum

Notes

Acknowledgments

The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico and the Fundação de Amparo à Pesquisa do Estado do Pará for financial support (Edital BIONORTE—Proc 552032/20120-7 and Edital VALE—Proc 2010/110447) and the Wellcome Trust for a grant to M.A. Ferguson-Smith. Sample collection was authorized by Instituto Brasileiro de Meio Ambiente e dos Recursos Nacionais Renováveis. We also thank the reviewers of the suggestions, especially the one related to the heterochromatin and the non-silencing of the autosome part of the Neo-X.

Supplementary material

10577_2013_9365_MOESM1_ESM.doc (99 kb)
ESM 1 (DOC 99 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • J. C. Pieczarka
    • 1
    • 2
    • 7
    Email author
  • A. J. B. Gomes
    • 1
    • 3
  • C. Y. Nagamachi
    • 1
    • 2
  • D. C. C. Rocha
    • 4
  • J. D. Rissino
    • 1
  • P. C. M. O’Brien
    • 5
  • F. Yang
    • 6
  • M. A. Ferguson-Smith
    • 5
  1. 1.Laboratório de Citogenética, ICBUFPABelemBrazil
  2. 2.CNPqBrasiliaBrazil
  3. 3.CNPq Doctoral Scholarship in Genetics and Molecular BiologyBrasiliaBrazil
  4. 4.Instituto Evandro ChagasBelémBrazil
  5. 5.Cambridge Resource Centre for Comparative GenomicsUniversity of CambridgeCambridgeUK
  6. 6.Cytogenetics FacilityWellcome Trust Sanger InstituteHinxtonUK
  7. 7.Instituto de Ciências BiológicasUniversidade Federal do ParáBelémBrazil

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