Chromosome Research

, Volume 21, Issue 4, pp 361–374 | Cite as

Molecular cytogenetic map of the central bearded dragon, Pogona vitticeps (Squamata: Agamidae)

  • M. J. Young
  • D. O’Meally
  • S. D. Sarre
  • A. Georges
  • T. Ezaz
Article

Abstract

Reptiles, as the sister group to birds and mammals, are particularly valuable for comparative genomic studies among amniotes. The Australian central bearded dragon (Pogona vitticeps) is being developed as a reptilian model for such comparisons, with whole-genome sequencing near completion. The karyotype consists of 6 pairs of macrochromosomes and 10 pairs microchromosomes (2n = 32), including a female heterogametic ZW sex microchromosome pair. Here, we present a molecular cytogenetic map for P. vitticeps comprising 87 anchor bacterial artificial chromosome clones that together span each macro- and microchromosome. It is the first comprehensive cytogenetic map for any non-avian reptile. We identified an active nucleolus organizer region (NOR) on the sub-telomeric region of 2q by mapping 18S rDNA and Ag-NOR staining. We identified interstitial telomeric sequences in two microchromosome pairs and the W chromosome, indicating that microchromosome fusion has been a mechanism of karyotypic evolution in Australian agamids within the last 21 to 19 million years. Orthology searches against the chicken genome revealed an intrachromosomal rearrangement of P. vitticeps 1q, identified regions orthologous to chicken Z on P. vitticeps 2q, snake Z on P. vitticeps 6q and the autosomal microchromosome pair in P. vitticeps orthologous to turtle Pelodiscus sinensis ZW and lizard Anolis carolinensis XY. This cytogenetic map will be a valuable reference tool for future gene mapping studies and will provide the framework for the work currently underway to physically anchor genome sequences to chromosomes for this model Australian squamate.

Keywords

Reptile Karyotype Microchromosome FISH Sex chromosome 

Abbreviations

APTX

Aprataxin

ATP5A1

ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle

BAC

Bacterial artificial chromosome

BCL6

B cell CLL/lymphoma 6

BLAST

Basic Local Alignment Search Tool

BLAT

BLAST-like alignment tool

CA10

Carbonic anhydrase X

CHD1

Chromodomain helicase DNA-binding protein 1

CTBP2

C-terminal binding protein 2

CTNNB1

Catenin (cadherin-associated protein), beta 1, 88 kDa

DAPI

4′,6-Diamidino-2-phenylindole

DDX58

DEAD (Asp-Glu-Ala-Asp) box polypeptide 58

DMRT1

Doublesex and mab-3-related transcription factor 1

dUTP

2′-Deoxyuridine 5′-triphosphate

EIF3H

Eukaryotic translation initiation factor 3, subunit H

FAM83B

Family with sequence similarity 83, member B

FBRSL1

Fibrosin-like 1

FISH

Fluorescence in situ hybridization

GHR

Growth hormone receptor

GMPPA

GDP-mannose pyrophosphorylase A

HCRTR2

Hypocretin (orexin) receptor 2

HMGCLL1

3-Hydroxymethyl-3-methylglutaryl-CoA lyase-like 1

IBSP

Integrin-binding sialoprotein

IPO7

Importin 7

IQSEC3

IQ motif and Sec7 domain 3

KAT2B

K(lysine) acetyltransferase 2B

KAT7

K(lysine) acetyltransferase 7

KLF6

Kruppel-like factor 6

NAV2

Neuron navigator 2

NOR

Nucleolus organizer region

NPRL3

Nitrogen permease regulator-like 3

PSMA2

Proteasome (prosome, macropain) subunit, alpha type, 2

RAB5A

RAB5A, member RAS oncogene family

rDNA

Ribosomal DNA

RRM1

Ribonucleotide reductase M1

SRY

Sex-determining region Y

TAX1BP1

Tax1 (human T-cell leukemia virus type I) binding protein 1

TMEM41B

Transmembrane protein 41B

TNFRSF11B

Tumor necrosis factor receptor superfamily, member 11b

TTN

Titin

WAC

WW domain-containing adaptor with coiled coil

ZNF143

Zinc finger protein 143

Notes

Acknowledgments

This work was funded by an ARC DP awarded to SD, AG and Scott Edwards, as was the purchase of the P. vitticeps BAC Library. This work was undertaken by MY as a Bachelor of Applied Science Honours with the Institute of Applied Ecology at the University of Canberra. We would like to thank Jacqui Richardson and Alistair Zealey for their care of captive animals and Juliet Ward for laboratory assistance.

Supplementary material

10577_2013_9362_MOESM1_ESM.doc (117 kb)
ESM 1(DOC 117 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • M. J. Young
    • 1
  • D. O’Meally
    • 1
  • S. D. Sarre
    • 1
  • A. Georges
    • 1
  • T. Ezaz
    • 1
  1. 1.Institute for Applied EcologyUniversity of CanberraCanberraAustralia

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