Interstitial telomeric repeats are enriched in the centromeres of chromosomes in Solanum species
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Interstitial telomeric repeats (ITRs) were reported in a number of animal and plant species. Most ITRs are organized as short tandem arrays and are likely evolutionary relics derived from chromosomal rearrangements and DNA repairs. However, megabase-sized ITR arrays were reported in Solanum species. Here, we report a fluorescence in situ hybridization (FISH) survey of ITRs in all representative diploid Solanum species, including potato, tomato, and eggplant. FISH revealed massive amplification of ITRs in the centromeric regions of chromosomes from the Solanum species containing the B and P genomes. A significant proportion of the ITR FISH signals was mapped within the primary constrictions of the pachytene chromosomes of Solanum pinnatisectum (B genome). In addition, some ITR sites overlapped with St49, a satellite repeat enriched in centromeric DNA sequences associated with CENH3 nucleosomes, in both A and B genome Solanum species. These results show that some ITR subfamilies have been amplified and invaded in the functional centromeres of chromosomes in Solanum species.
Keywordsinterstitial telomeric repeats centromere potato Solanum fluorescence in situ hybridization
Interstitial telomeric repeats
Fluorescence in situ hybridization
Bacterial artificial chromosome
We thank Dr. Christian Bachem, Wageningen University, the Netherlands, for providing the potato BAC clones. This work was supported by grant 2009CDA085 from the Natural Science Foundation of Hubei Province and grant 2006AA100107 from the National High-Tech Program of China to L.J., and by grant IOS1237969 from the National Science Foundation (USA) to J.J. G.A.T. was supported by a CAPES fellowship from the Brazilian Ministry of Education, Brazil.
- Gong ZY, Wu YF, Koblížková A, Torres GA, Wang K, Iovene M, Neumann P, Zhang WL, Novák P, Buell CR, Macas J, Jiang JM (2012) Repeatless and repeat-based centromeres in potato: implications for centromere evolution. Plant Cell 24:3559–3574Google Scholar
- Mendez-Lago M, Wild J, Whitehead SL, Tracey A, de Pablos B, Rogers J, Szybalski W, Villasante A (2009) Novel sequencing strategy for repetitive DNA in a Drosophila BAC clone reveals that the centromeric region of the Y chromosome evolved from a telomere. Nucleic Acids Res 37:2264–2273PubMedCrossRefGoogle Scholar
- Tang XM, Szinay D, Lang C, Ramanna MS, van der Vossen EAG, Datema E, Lankhorst RK, de Boer J, Peters SA, Bachem C, Stiekema W, Visser RGF, de Jong H, Bai YL (2008) Cross-species bacterial artificial chromosome–fluorescence in situ hybridization painting of the tomato and potato chromosome 6 reveals undescribed chromosomal rearrangements. Genetics 180:1319–1328PubMedCrossRefGoogle Scholar
- Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160PubMedGoogle Scholar