Chromosome Research

, Volume 18, Issue 6, pp 655–666 | Cite as

Genome structure affects the rate of autosyndesis and allosyndesis in AABC, BBAC and CCAB Brassica interspecific hybrids

  • Annaliese S. Mason
  • Virginie Huteau
  • Frédérique Eber
  • Olivier Coriton
  • Guijun Yan
  • Matthew N. Nelson
  • Wallace A. Cowling
  • Anne-Marie Chèvre
Article

Abstract

Gene introgression into allopolyploid crop species from diploid or polyploid ancestors can be accomplished through homologous or homoeologous chromosome pairing during meiosis. We produced trigenomic Brassica interspecific hybrids (genome complements AABC, BBAC and CCAB) from the amphidiploid species Brassica napus (AACC), Brassica juncea (AABB) and Brassica carinata (BBCC) in order to test whether the structure of each genome affects frequencies of homologous and homoeologous (both allosyndetic and autosyndetic) pairing during meiosis. AABC hybrids produced from three genotypes of B. napus were included to assess the genetic control of homoeologous pairing. Multi-colour fluorescent in situ hybridisation was used to quantify homologous pairing (e.g. A-genome bivalents in AABC), allosyndetic associations (e.g. B–C in AABC) and autosyndetic associations (e.g. B–B in AABC) at meiosis. A high percentage of homologous chromosomes formed pairs (97.5–99.3%), although many pairs were also involved in autosyndetic and allosyndetic associations. Allosyndesis was observed most frequently as A–C genome associations (mean 4.0 per cell) and less frequently as A–B genome associations (0.8 per cell) and B–C genome associations (0.3 per cell). Autosyndesis occurred most frequently in the haploid A genome (0.75 A–A per cell) and least frequently in the haploid B genome (0.13 B–B per cell). The frequency of C–C autosyndesis was greater in BBAC hybrids (0.75 per cell) than in any other hybrid. The frequency of A–B, A–C and B–C allosyndesis was affected by the genomic structure of the trigenomic hybrids. Frequency of allosyndesis was also influenced by the genotype of the B. napus paternal parent for the three AABC (B. juncea × B. napus) hybrid types. Homoeologous pairing between the Brassica A, B and C genomes in interspecific hybrids may be influenced by complex interactions between genome structure and allelic composition.

Keywords

allosyndesis autosyndesis meiosis Brassica interspecific hybrid fluorescent in situ hybridisation 

Abbreviations

BAC

Bacterial artificial chromosome; a DNA construct inserted into a bacterial plasmid

DAPI

4′,6-Diamidino-2-phenylindole; a blue fluorescent stain that binds strongly to DNA

FISH

Fluorescent in situ hybridisation; a cytogenetic technique used to detect the presence or absence of specific DNA sequences on chromosomes

GISH

Genomic in situ hybridisation; a specific type of FISH whereby genomic DNA is used as a probe

PMC/s

Pollen mother cell/s. Cells produced by the male generative organs in plants which subsequently undergo meiosis and cell division to develop into (usually) four haploid pollen grains

Notes

Acknowledgements

Travel of the first author was sponsored by the Mike Carroll Travelling Fellowship and a Convocation Postgraduate Research Travel Award from The University of Western Australia (Perth, Australia). This research was funded by INRA (Institut National de la Recherche Agronomique, Le Rheu, France) and Australian Research Council Linkage Project LP0667805 with industry partners Norddeutsche Pflanzenzucht Hans-Georg Lembke KG (Germany) and the Council of Grain Grower Organisations Ltd (Australia).

Supplementary material

10577_2010_9140_MOESM1_ESM.doc (78 kb)
Supplementary Table 4Frequency of observations (mean number per cell and range) for various chromosome configurations at meiosis I in 40 pollen mother cells (PMCs) of trigenomic Brassica interspecific hybrids created from crosses between B. juncea, B. napus and B. carinata. Multi-colour FISH was used to label chromosomes in the Brassica A, B and C genomes (n = 10, n = 8 and n = 9 chromosomes, respectively). AABC-1, AABC-2 and AABC-3 result from crosses between three different B. napus cultivars (doubled haploid derivatives of ‘Surpass400’, ‘Monty’ and ‘Westar’, respectively) and a single B. juncea genotype. (DOC 78 kb)

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Annaliese S. Mason
    • 1
  • Virginie Huteau
    • 2
  • Frédérique Eber
    • 2
  • Olivier Coriton
    • 2
  • Guijun Yan
    • 1
  • Matthew N. Nelson
    • 1
  • Wallace A. Cowling
    • 1
  • Anne-Marie Chèvre
    • 2
  1. 1.School of Plant Biology and Institute of AgricultureThe University of Western AustraliaCrawleyAustralia
  2. 2.UMR Amélioration des Plantes et Biotechnologies Végétales, INRALe Rheu CedexFrance

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