Chromosome Research

, Volume 18, Issue 2, pp 287–299 | Cite as

Integration of cytogenetic and genetic linkage maps of Lotus japonicus, a model plant for legumes

  • Nobuko Ohmido
  • Akiko Ishimaru
  • Seiji Kato
  • Shusei Sato
  • Satoshi Tabata
  • Kiichi Fukui
Article

Abstract

To construct a high-resolution pachytene chromosome map, we used the chromosome image analyzing system version 3 and fluorescence in situ hybridization. Two ribosomal RNA genes (45S rDNA and 5S rDNA), two major tandem repeat DNAs (LjTR1 and LjTR2), two major retroelements (LjRE1 and LjRE2), and 27 transformation-competent artificial chromosome clones were physically localized on Lotus japonicus (Miyakojima MG-20, 2n = 12) chromosomes. The distributions of heterochromatin and euchromatin along six chromosomes were compared based on the linkage map. Distortion between the recombination frequencies and physical chromosomal distance was recognized where the centromeric heterochromatic regions and constitutive heterochromatin are composed of the highest copy tandem repeat LjTR1 on the interstitial specific regions. Our study shows that the heterochromatin are composed of the specific repeated sequences, and the discrepancy between the recombination frequency and cytological information detected in L. japonicus chromosomes is due to the heterochromatin.

Keywords

Lotus japonicus L. CHIAS3 pachytene chromosome FISH integration map heterochromatin 

Abbreviations

CHIAS

chromosome image analyzing system

dCAPS

derived cleaved amplified polymorphic sequences

DAPI

4',6-diamidino-2-phenylindole

FISH

fluorescence in situ hybridization

LG

linkage group

LjRE

Louts japonicus retroelements

LjTR

Louts japonicus tandem repeat DNA

LTRs

long terminal repeats

PCR

polymerase chain reaction

rDNA

ribosomal RNA gene

SA

streptavidin

SSR

simple sequence repeat

TAC

transformation-competent artificial chromosome

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Nobuko Ohmido
    • 1
  • Akiko Ishimaru
    • 1
  • Seiji Kato
    • 2
  • Shusei Sato
    • 3
  • Satoshi Tabata
    • 3
  • Kiichi Fukui
    • 4
  1. 1.Graduate School of Human Development and EnvironmentKobe UniversityKobeJapan
  2. 2.Yamanashi Prefectural Agritechnology CenterYamanashiJapan
  3. 3.Kazusa DNA Research InstituteKisarazuJapan
  4. 4.Department of Biotechnology, Graduate School of EngineeringOsaka UniversitySuitaJapan

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