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Chromosome Research

, 17:863 | Cite as

Unstable transmission of rice chromosomes without functional centromeric repeats in asexual propagation

  • Zhiyun Gong
  • Hengxiu Yu
  • Jian Huang
  • Chuandeng Yi
  • Minghong GuEmail author
Article

Abstract

During sexual propagation of primary trisomic 8, chromosome 8 breaks in some rice plants, resulting in a telotrisomic (2n+·8S) line. In this study, we observed that the extra short arm of chromosome 8 (·8S) can easily be lost in the telotrisomic, and we determined by fluorescence in-situ hybridization (FISH) analysis that the centromeric region of the extra ·8S did not contain the rice centromeric satellite repeat (CentO) and centromere-specific retrotransposon (CRR); however, the extra ·8S contained part of the CentO and CRR sequences in the initially preserved telotrisomic line. We confirmed by real-time quantitative PCR (RQ-PCR) analysis that the original functional centromere of the extra ·8S was lost. Using both FISH and RQ-PCR, the breakage point of the extra ·8S was found within the BAC clone a0070J19 sequence containing the first part of the short arm near the centromere region of chromosome 8 but without any CentO or CRR sequences. However, part of the DNA sequence within the a0070J19 BAC clone played a role in the new functional centromere, contributing to the morphological variations by asexually propagated plants of rice telotrisomics in the field. We conclude that CENH3, a key element in the eukaryotic kinetochore, may not always bind properly with the new functional centromere, resulting in loss of the extra ·8S during mitosis and the chromosome numbers returning to diploid levels in subsequent generations.

Keywords

rice neocentromere CENH3 asexual propagation 

Abbreviations

BAC

bacterial artificial chromosome

CentO

centromeric satellite repeat

CRR

centromere-specific retrotransposon

DAPI

4′,6-diamidinophenylindole

FISH

fluorescence in-situ hybridization

FITC

fluorescein isothiocyanate

PMC

pollen mother cell

RQ-PCR

real-time quantitative PCR

Notes

Acknowledgements

We are grateful to Zhukuan Cheng for critical reading of the manuscript and Yong Zhou for RQ-PCR analysis. This work was supported by grants from the National Natural Science Foundation of China (30600345 and 30770131).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Zhiyun Gong
    • 1
  • Hengxiu Yu
    • 1
  • Jian Huang
    • 2
  • Chuandeng Yi
    • 1
  • Minghong Gu
    • 1
    Email author
  1. 1.Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of EducationYangzhou UniversityYangzhouChina
  2. 2.Laboratory of Genetics, School of Basic Medicine & Biological SciencesSuzhou UniversitySuzhouChina

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