Chromosome Research

, Volume 17, Issue 3, pp 419–436 | Cite as

Cross-species chromosome painting in Cetartiodactyla: Reconstructing the karyotype evolution in key phylogenetic lineages

  • Anastasia I. Kulemzina
  • Vladimir A. Trifonov
  • Polina L. Perelman
  • Nadezhda V. Rubtsova
  • Vitaly Volobuev
  • Malcolm A. Ferguson-Smith
  • Roscoe Stanyon
  • Fengtang Yang
  • Alexander S. Graphodatsky
Article

Abstract

Recent molecular and morphological studies place Artiodactyla and Cetacea into the order Cetartiodactyla. Within the Cetartiodactyla such families as Bovidae, Cervidae, and Suidae are well studied by comparative chromosome painting, but many taxa that are crucial for understanding cetartiodactyl phylogeny remain poorly studied. Here we present the genome-wide comparative maps of five cetartiodactyl species obtained by chromosome painting with human and dromedary paint probes from four taxa: Cetacea, Hippopotamidae, Giraffidae, and Moschidae. This is the first molecular cytogenetic report on pilot whale, hippopotamus, okapi, and Siberian musk deer. Our results, when integrated with previously published comparative chromosome maps allow us to reconstruct the evolutionary pathway and rates of chromosomal rearrangements in Cetartiodactyla. We hypothesize that the putative cetartiodactyl ancestral karyotype (CAK) contained 25–26 pairs of autosomes, 2n = 52–54, and that the association of human chromosomes 8/9 could be a cytogenetic signature that unites non-camelid cetartiodactyls. There are no unambiguous cytogenetic landmarks that unite Hippopotamidae and Cetacea. If we superimpose chromosome rearrangements on the supertree generated by Price and colleagues, several homoplasy events are needed to explain cetartiodactyl karyotype evolution. Our results apparently favour a model of non-random breakpoints in chromosome evolution. Cetariodactyl karyotype evolution is characterized by alternating periods of low and fast rates in various lineages. The highest rates are found in Suina (Suidae+Tayasuidae) lineage (1.76 rearrangements per million years (R/My)) and the lowest in Cetaceans (0.07 R/My). Our study demonstrates that the combined use of human and camel paints is highly informative for revealing evolutionary karyotypic rearrangements among cetartiodactyl species.

Keywords

cetaceans Pecora hippopotamus breakpoint reuse Zoo-FISH chromosome evolution 

Abbreviations

2n

diploid number of chromosomes

BAC

bacterial artificial chromosome

BTA

Bos taurus

CAK

cetartiodactyl ancestral karyotype

CDR

Camelus dromedarius

DOP-PCR

degenerate oligonucleotide primer–polymerase chain reaction

FISH

fluorescence in-situ hybridization

GCA

Giraffa camelopardalis

GME

Globicephala melas

GTG

banding G banding by trypsin using Giemsa

HAM

Hippopotamus amphibius

HSA

Homo sapiens

MMO

Moschus moschiferus

Mya

million years ago

OJO

Okapia johnstoni

PAUP

phylogenetic analysis using parsimony

PTA

Pecari tajacu

R/My

rearrangements per million years

SSC

Sus scrofa

Supplementary material

10577_2009_9032_Fig1_ESM.jpg (1.3 mb)
Supplementary Fig. S1

FISH examples of localization of some human (HSA) and dromedary (CDR) probes onto studied species (JPG 1.29 MB)

10577_2009_9032_Fig2_ESM.jpg (572 kb)
Supplementary Fig. S2

FISH of dromedary (CDR) painting probes onto cow (BTA) and pig (SSC), with additional signals revealed (JPG 571 kb)

10577_2009_9032_Fig3_ESM.jpg (1.2 mb)
Supplementary Fig. S3

Supplementary figure S3 Schemes of possible chromosome rearrangements for two conserved associations: CDR 23/21/9/13 and CDR 4/31 (JPG 1.21 MB)

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Anastasia I. Kulemzina
    • 1
  • Vladimir A. Trifonov
    • 1
  • Polina L. Perelman
    • 1
    • 2
  • Nadezhda V. Rubtsova
    • 1
  • Vitaly Volobuev
    • 3
  • Malcolm A. Ferguson-Smith
    • 4
  • Roscoe Stanyon
    • 5
  • Fengtang Yang
    • 6
  • Alexander S. Graphodatsky
    • 1
  1. 1.Institute of Cytology and GeneticsRussian Academy of SciencesNovosibirskRussia
  2. 2.National Cancer InstituteFrederickUSA
  3. 3.Museum National d’Histoire NaturelleOrigine, Structure et Evolution de la BiodiversiteParisFrance
  4. 4.Department of Veterinary MedicineCambridge Resource Centre for Comparative GenomicsCambridgeUK
  5. 5.Department of Evolutionary BiologyUniversity of FlorenceFlorenceItaly
  6. 6.The Wellcome Trust Sanger InstituteWellcome Trust Genome CampusCambridgeUK

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