Chromosome Research

, 17:11 | Cite as

Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria

  • María Teruel
  • Josefa Cabrero
  • Eugenia E. Montiel
  • Manuel J. Acosta
  • Antonio Sánchez
  • Juan Pedro M. Camacho
Article

Abstract

Acquisition of knowledge of the nature and DNA content of B chromosomes has been triggered by a collection of molecular techniques, one of which, microdissection, has provided interesting results in a number of B chromosome systems. Here we provide the first data on the molecular composition of B chromosomes in Locusta migratoria, after microdissection of the B and X chromosomes, DNA amplification by one (B) or two (X) different methods, and chromosome painting. The results showed that B chromosomes share at least two types of repetitive DNA sequences with the A chromosomes, suggesting that Bs in this species most likely arose intraspecifically. One of these repetitive DNAs is located on the heterochromatic distal half of the B chromosome and in the pericentromeric regions of about half of the A chromosomes, including the X. The other type of repetitive DNA is located interspersedly over the non-centromeric euchromatic regions of all A chromosomes and in an interstitial part of the proximal euchromatic half of the B chromosome. Chromosome painting, however, did not provide results sufficiently reliable to determine, in this species, which A chromosome gave rise to the B; this might be done by detailed analysis of the microdissected DNA sequences

Keywords

B chromosome chromosome painting locust Locusta migratoria microdissection 

Abbreviations

AFLP

amplified fragment length polymorphism

CMA3

chromomycin A3

DAPI

4′,6-diamidino-2-phenylindole

DOP-PCR

degenerate oligonucleotide primed PCR

Notes

Acknowledgements

We thank David Martinez for language corrections, Tatiana López for technical assistance, and W. Traut and two anonymous reviewers for their perceptive criticism. This study was supported by grants from the Spanish Ministerio de Ciencia y Tecnología (CGL2006-06307 and CGL2006-05308/BOS, cofunded by the European Regional Development Fund) and Plan Andaluz de Investigación (P06-CVI-1664, BIO 165 and BIO 220).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • María Teruel
    • 1
  • Josefa Cabrero
    • 1
  • Eugenia E. Montiel
    • 1
  • Manuel J. Acosta
    • 2
  • Antonio Sánchez
    • 2
  • Juan Pedro M. Camacho
    • 1
  1. 1.Departamento de GenéticaUniversidad de GranadaGranadaSpain
  2. 2.Departamento de Biología ExperimentalUniversidad de JaénAndalusiaSpain

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