Chromosome Research

, Volume 16, Issue 8, pp 1143–1158 | Cite as

Comparing chromosomal and mitochondrial phylogenies of sportive lemurs (Genus Lepilemur, Primates)

  • Yves Rumpler
  • Stephanie Warter
  • Marcel Hauwy
  • Jean-Luc Fausser
  • Christian Roos
  • Dietmar Zinner


In recent years several new sportive lemur species (genus Lepilemur) have been described. In contrast to other lemur taxa, the genus shows comparatively high chromosomal variability, which, in addition to molecular data, can be used to infer phylogenetic relationships within the genus. By comparing R-banding patterns and fluorescence in-situ hybridization data, we detected chromosomal rearrangements that occurred during speciation within the genus. The analysis of these data with cladistic methods resulted in a dichotomic phylogenetic tree comparable to that obtained from mitochondrial sequence data. However, a phase of reticulation can not be excluded from the evolution of Lepilemur. Although some incongruences were detected, both phylogenies show similar patterns concerning relationships of the basal and terminal splits. We therefore hypothesize that both, chromosome rearrangements and molecular mutations, alone or in combination, contributed to the speciation process in sportive lemurs.

Key words

cytogenetics fluorescent in-situ hybridization Lepilemur mitochondria phylogeny sportive lemurs 



charge-coupled device




fluorescence in-situ hybridization


fluorescein isothiocyanate


Homo sapiens


maximum parsimony


Lepilemur ankaranensis


Lepilemur dorsalis


Lepilemur edwardsi


ancestor of the genus Lepilemur


Lepilemur leucopus


Lepilemur microdon


Lepilemur mittermeieri


Lepilemur ruficaudatus


Lepilemur septentrionalis


Microcebus murinus


phylogenetic analysis using parsimony



We thank the Direction des Eaux et Forêts of Antananarivo and Mahajanga, the Association Nationale pour la Gestion des Aires Protégées of Antananarivo and Mahajanga for their permission to do the field work. We thank the Faculty of Sciences of Antananarivo and the Institut Pasteur of Antananarivo for their support. We thank M. Lavaux for their technical assistance and V. Volobouev for his fruitful help with cladistic phylogeny. Grants came from A.E.E.C.L. and ULP-EA34280.

Supplementary material

10577_2008_1265_MOESM1_ESM.xls (26 kb)
(XLS 25.5 kb)


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Yves Rumpler
    • 1
  • Stephanie Warter
    • 1
  • Marcel Hauwy
    • 1
  • Jean-Luc Fausser
    • 1
  • Christian Roos
    • 2
  • Dietmar Zinner
    • 3
  1. 1.Institut d’EmbryologieUniversité Louis Pasteur, Faculté de Médecine-EA3428StrasbourgFrance
  2. 2.Primate Genetics and Gene Bank of PrimatesDeutsches PrimatenzentrumGöttingenGermany
  3. 3.Cognitive EthologyDeutsches PrimatenzentrumGöttingenGermany

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