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Chromosome Research

, Volume 16, Issue 8, pp 1143–1158 | Cite as

Comparing chromosomal and mitochondrial phylogenies of sportive lemurs (Genus Lepilemur, Primates)

  • Yves Rumpler
  • Stephanie Warter
  • Marcel Hauwy
  • Jean-Luc Fausser
  • Christian Roos
  • Dietmar Zinner
Article

Abstract

In recent years several new sportive lemur species (genus Lepilemur) have been described. In contrast to other lemur taxa, the genus shows comparatively high chromosomal variability, which, in addition to molecular data, can be used to infer phylogenetic relationships within the genus. By comparing R-banding patterns and fluorescence in-situ hybridization data, we detected chromosomal rearrangements that occurred during speciation within the genus. The analysis of these data with cladistic methods resulted in a dichotomic phylogenetic tree comparable to that obtained from mitochondrial sequence data. However, a phase of reticulation can not be excluded from the evolution of Lepilemur. Although some incongruences were detected, both phylogenies show similar patterns concerning relationships of the basal and terminal splits. We therefore hypothesize that both, chromosome rearrangements and molecular mutations, alone or in combination, contributed to the speciation process in sportive lemurs.

Key words

cytogenetics fluorescent in-situ hybridization Lepilemur mitochondria phylogeny sportive lemurs 

Abbreviations

CCD

charge-coupled device

DAPI

4′,6-diamidino-2-phenylindole

FISH

fluorescence in-situ hybridization

FITC

fluorescein isothiocyanate

HSA

Homo sapiens

MP

maximum parsimony

LAN

Lepilemur ankaranensis

LDO

Lepilemur dorsalis

LED

Lepilemur edwardsi

LEPI

ancestor of the genus Lepilemur

LLE

Lepilemur leucopus

LMI

Lepilemur microdon

LMT

Lepilemur mittermeieri

LRU

Lepilemur ruficaudatus

LSE

Lepilemur septentrionalis

MMR

Microcebus murinus

PAUP

phylogenetic analysis using parsimony

Notes

Acknowledgements

We thank the Direction des Eaux et Forêts of Antananarivo and Mahajanga, the Association Nationale pour la Gestion des Aires Protégées of Antananarivo and Mahajanga for their permission to do the field work. We thank the Faculty of Sciences of Antananarivo and the Institut Pasteur of Antananarivo for their support. We thank M. Lavaux for their technical assistance and V. Volobouev for his fruitful help with cladistic phylogeny. Grants came from A.E.E.C.L. and ULP-EA34280.

Supplementary material

10577_2008_1265_MOESM1_ESM.xls (26 kb)
(XLS 25.5 kb)

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Yves Rumpler
    • 1
  • Stephanie Warter
    • 1
  • Marcel Hauwy
    • 1
  • Jean-Luc Fausser
    • 1
  • Christian Roos
    • 2
  • Dietmar Zinner
    • 3
  1. 1.Institut d’EmbryologieUniversité Louis Pasteur, Faculté de Médecine-EA3428StrasbourgFrance
  2. 2.Primate Genetics and Gene Bank of PrimatesDeutsches PrimatenzentrumGöttingenGermany
  3. 3.Cognitive EthologyDeutsches PrimatenzentrumGöttingenGermany

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