Chromosome Research

, 16:587 | Cite as

Centromere repositioning in the X chromosome of XO/XO mammals, Ryukyu spiny rat

  • Tsuyoshi Kobayashi
  • Fumio Yamada
  • Takuma Hashimoto
  • Shintaro Abe
  • Yoichi Matsuda
  • Asato KuroiwaEmail author


Two species of Ryukyu spiny rat, Tokudaia osimensis and Tokudaia tokunoshimensis, have an XO/XO sex chromosome constitution with no cytogenetically visible Y chromosome in both sexes. The single X chromosomes of T. osimensis and T. tokunoshimensis are submetacentric and subtelocentric, respectively. It was therefore suggested that a pericentric inversion event occurred in the X chromosome of either species. To identify X chromosome rearrangements that have occurred between the two species, we mapped 22 mouse cDNA clones of the X-linked genes on the chromosomes of the two species by direct R-banding FISH. The gene orders of the X chromosomes were conserved in the two species, whereas the position of the centromere on the X chromosome was different. This result indicates that the rearrangement which occurred in either of the X chromosomes after the two species diverged from a common ancestor involved not pericentric inversion but centromere repositioning.

Key words

CR comparative mapping FISH Tokudaia osimensis Tokudaia tokunoshimensis 


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Copyright information

© Springer 2008

Authors and Affiliations

  • Tsuyoshi Kobayashi
    • 1
  • Fumio Yamada
    • 2
  • Takuma Hashimoto
    • 3
  • Shintaro Abe
    • 4
  • Yoichi Matsuda
    • 1
    • 5
  • Asato Kuroiwa
    • 1
    • 5
    Email author
  1. 1.Laboratory of Animal Cytogenetics, Graduate School of ScienceHokkaido UniversityKita-kuJapan
  2. 2.Kansai Research CenterForestry and Forest Products Research InstituteMomoyamaJapan
  3. 3.Japan Wild Life Research CenterTaitoh-kuJapan
  4. 4.Naha Nature Conservation OfficeMinistry of the EnvironmentNaha OkinawaJapan
  5. 5.Laboratory of Animal Cytogenetics, Creative Research Initiative “Sousei”Hokkaido UniversityKita-kuJapan

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