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Chromosome Research

, Volume 16, Issue 1, pp 129–143 | Cite as

Phylogenomics of the dog and fox family (Canidae, Carnivora) revealed by chromosome painting

  • Alexander S. Graphodatsky
  • Polina L. Perelman
  • Natalya V. Sokolovskaya
  • Violetta R. Beklemisheva
  • Natalya A. Serdukova
  • Gauthier Dobigny
  • Stephen J. O’Brien
  • Malcolm A. Ferguson-Smith
  • Fengtang Yang
Article

Abstract

Canid species (dogs and foxes) have highly rearranged karyotypes and thus represent a challenge for conventional comparative cytogenetic studies. Among them, the domestic dog is one of the best-mapped species in mammals, constituting an ideal reference genome for comparative genomic study. Here we report the results of genome-wide comparative mapping of dog chromosome-specific probes onto chromosomes of the dhole, fennec fox, and gray fox, as well as the mapping of red fox chromosome-specific probes onto chromosomes of the corsac fox. We also present an integrated comparative chromosome map between the species studied here and all canids studied previously. The integrated map demonstrates an extensive conservation of whole chromosome arms across different canid species. In addition, we have generated a comprehensive genome phylogeny for the Canidae on the basis of the chromosome rearrangements revealed by comparative painting. This genome phylogeny has provided new insights into the karyotypic relationships among the canids. Our results, together with published data, allow the formulation of a likely Canidae ancestral karyotype (CAK, 2n = 82), and reveal that at least 6–24 chromosomal fission/fusion events are needed to convert the CAK karyotype to that of the modern canids.

Key words

Canidae Carnivora chromosome maps chromosome painting evolution genome phylogeny 

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Supplementary material

10577_2007_1203_Fig1_ESM.jpg (501 kb)
Supplementary Figure S1 Diploid G-banded karyotype of fennec fox and gray fox. Summary of hybridization pattern of domestic dog probes onto G-banded fennec fox and gray fox chromosomes (JPEG 501 kb)
10577_2007_1203_Fig2_ESM.jpg (770 kb)
Supplementary Figure S2 A single tree retrieved from PAUP analysis of 88 characters rooted on bears, with changes mapped onto the topology. Bootstrap values are provided above branches (JPEG 769 kb)
10577_2007_1203_MOESM3_ESM.xls (96 kb)
Supplementary Table S1 Matrix of chromosomal characters corresponding to presence (1) or absence (0) of segmental associations of dog chromosomal fragments (XLS 96 kb)

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Copyright information

© Springer 2008

Authors and Affiliations

  • Alexander S. Graphodatsky
    • 1
  • Polina L. Perelman
    • 1
    • 2
  • Natalya V. Sokolovskaya
    • 1
  • Violetta R. Beklemisheva
    • 1
  • Natalya A. Serdukova
    • 1
  • Gauthier Dobigny
    • 3
  • Stephen J. O’Brien
    • 2
  • Malcolm A. Ferguson-Smith
    • 4
  • Fengtang Yang
    • 5
  1. 1.Institute of Cytology and Genetics, SB RASNovosibirskRussia
  2. 2.Laboratory of Genomic DiversityNational Cancer InstituteFrederickUSA
  3. 3.Institut de Recherche pour le Développement, Centre de Biologie et Gestion des PopulationsCampus International de Baillarguet, CS30016Montferrier-sur-LezFrance
  4. 4.Cambridge Resource Centre for Comparative Genomics, Department of Veterinary MedicineUniversity of CambridgeCambridgeUK
  5. 5.The Wellcome Trust Sanger InstituteCambridgeUK

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