Chromosome Research

, Volume 16, Issue 1, pp 89–107 | Cite as

Multidirectional cross-species painting illuminates the history of karyotypic evolution in Perissodactyla

  • Vladimir A. Trifonov
  • Roscoe Stanyon
  • Anastasia I. Nesterenko
  • Beiyuan Fu
  • Polina L. Perelman
  • Patricia C M O’Brien
  • Gary Stone
  • Nadezhda V. Rubtsova
  • Marlys L. Houck
  • Terence J. Robinson
  • Malcolm A. Ferguson-Smith
  • Gauthier Dobigny
  • Alexander S. Graphodatsky
  • Fengtang Yang
Article

Abstract

The order Perissodactyla, the group of odd-toed ungulates, includes three extant families: Equidae, Tapiridae, and Rhinocerotidae. The extremely rapid karyotypic diversification in perissodactyls has so far prevented the establishment of genome-wide homology maps between these three families by traditional cytogenetic approaches. Here we report the first genome-wide comparative chromosome maps of African rhinoceroses, four tapir species, four equine species, and humans. These maps were established by multidirectional chromosome painting, with paint probes derived from flow-sorted chromosomes of Equus grevyi, Tapirus indicus, and Ceratotherium simum as well as painting probes from horse and human. The Malayan tapir (Tapirus indicus), Baird’s tapir (T. bairdii), mountain tapir (T. pinchaque), lowland tapir (T. terrestris), and onager (E. hemionus onager), were studied by cross-species chromosome painting for the first time. Our results, when integrated with previously published comparative chromosome maps of the other perissodactyl species, have enabled the reconstruction of perissodactyl, ceratomorph, and equid ancestral karyotypes, and the identification of the defining evolutionary chromosomal rearrangements along each lineage. Our results allow a more reliable estimate of the mode and tempo of evolutionary chromosomal rearrangements, revealing a striking switch between the slowly evolving ceratomorphs and extremely rapidly evolving equids.

Key words

chromosomal evolution chromosome painting cladistics Equidae karyology Perissodactyla 

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Supplementary material

10577_2007_1201_MOESM1_ESM.doc (154 kb)
Supplementary Table S1Matrix of human-based (HSA) and horse-based (ECA) chromosomal characters. EPR, EHE, EAS, EZE, EGR, EBU, CSI, DBI, TIN, TBA, TTE, TPI represent Przewalski’s horse, onager, donkey, mountain zebra, Grevy’s zebra, Burchell’s zebra, white rhinoceros, black rhinoceros, Malayan tapir, Baird’s tapir, lowland tapir, and mountain tapir respectively. Partial chromosomes/chromosome arms are marked by inverted commas. “Inv” indicates inversion (DOC 154 kb)
10577_2007_1201_Fig1_ESM.jpg (215 kb)
Supplementary Figure S2Karyotype of Grevy’s zebra with human (HSA) and Malayan tapir (TIN) homologies. Human homologies that were not found in these experiments, but are inferred from previously published data are indicated by circles (JPEG 214 kb)

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Copyright information

© Springer 2008

Authors and Affiliations

  • Vladimir A. Trifonov
    • 1
    • 2
  • Roscoe Stanyon
    • 3
  • Anastasia I. Nesterenko
    • 1
  • Beiyuan Fu
    • 2
  • Polina L. Perelman
    • 1
    • 4
  • Patricia C M O’Brien
    • 2
  • Gary Stone
    • 4
  • Nadezhda V. Rubtsova
    • 1
  • Marlys L. Houck
    • 5
  • Terence J. Robinson
    • 6
  • Malcolm A. Ferguson-Smith
    • 2
  • Gauthier Dobigny
    • 7
  • Alexander S. Graphodatsky
    • 1
  • Fengtang Yang
    • 8
  1. 1.Institute of Cytology and GeneticsRussian Academy of SciencesNovosibirskRussia
  2. 2.Department of Veterinary MedicineCambridge Resource Centre for Comparative GenomicsCambridgeUK
  3. 3.Department of Animal Biology and GeneticsUniversity of FlorenceFlorenceItaly
  4. 4.National Cancer InstituteFrederickUSA
  5. 5.Conservation and Research for Endangered SpeciesZoological Society of San DiegoSan DiegoUSA
  6. 6.Evolutionary Genomics Group, Department of Botany and ZoologyUniversity of StellenboschMatielandSouth Africa
  7. 7.Institut de Recherche pour le Développement, Centre de Biologie et Gestion des PopulationsCampus International de Baillarguet, CS30016Montferrier-sur-LezFrance
  8. 8.The Wellcome Trust Sanger InstituteWellcome Trust Genome CampusCambridgeUK

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