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Chromosome Research

, 15:115 | Cite as

Karyotype diversity and the origin of grapefruit

  • Ana Paula de Moraes
  • Walter dos Santos Soares Filho
  • Marcelo Guerra
Article

Abstract

Grapefruit is a group of citrus of recent origin, probably resulting from a cross between pummelo and sweet-orange. Aiming to investigate this putative origin and the genetic variability among grapefruit cultivars, the karyotype of six grapefruits, two pummelos, and one tangelo cultivar (grapefruit × tangerine) were analyzed using sequential CMA/DAPI double staining and FISH with rDNA probes. The karyotypes of grapefruit ‘Duncan’ and ‘Foster’ differ from those of ‘Flame’, ‘Henderson’, ‘Marsh’ and ‘Rio Red’. The former have two chromosomes with a single CMA+ band in both terminal regions (C type chromosome) and six chromosomes with only one CMA+ terminal band (D type), whereas the latter have three C and five D type chromosomes. All accessions investigated exhibited two chromosomes with 5S rDNA but a variable number of 45S rDNA. The two former grapefruits displayed four 45S rDNA sites, whereas the remaining grapefruit cultivars had five. The two pummelos showed identical karyotypes, homozygous for CMA+ bands and their four rDNA sites. From each pummelo chromosome pair one chromosome seems to be present in grapefruit karyotypes. The different grapefruit karyotypes might result from independent crosses between pummelos of different karyotypic constitution and sweet-oranges. The chromosome markers found in the tangelo ‘Orlando’ and the position of their two 45S rDNA confirm the grapefruit ‘Duncan’ and the tangerine ‘Dancy’ as their parents.

Key words

Citrus CMA/DAPI banding grapefruit karyotype evolution rDNA 

References

  1. Barrett HC, Rhodes AM (1976) A numerical taxonomic study of affinity relationships in cultivated Citrus and its close relatives. Syst Bot 1: 105–136.CrossRefGoogle Scholar
  2. Befu M, Kitajima A, Ling YX, Hasegawa K (2000) Classification of ‘Tosa-Butan’ pummelo (Citrus grandis [L.] Osb.), ‘Washington’ Navel orange (C. sinensis [L.] Osb.) and trifoliate orange (Poncirus trifoliata [L.] Raf.) chromosomes using young leaves. J Jpn Soc Hort Sci 69: 22–28.Google Scholar
  3. Befu M, Kitajima A, Hasegawa K (2001) Chromosome composition of some citrus species and cultivars based on the chromomycin A3 (CMA) banding patterns. J Jpn Soc Hort Sci 70: 83–88.Google Scholar
  4. Befu M, Kitajima A, Hasegawa K (2002) Classification of the Citrus chromosomes with same types of chromomycin A banding patterns. J Jpn Soc Hort Sci 71: 394–400.Google Scholar
  5. Bowman KD, Gmitter FG, Jr (1990) Caribbean Forbidden Fruit: grapefruit’s missing link with the past and bridge to the future? Fruit Var J 44: 41–44.Google Scholar
  6. Carvalho R, Soares Filho WS, Brasileiro-Vidal AC, Guerra M (2005) The relationship among lemons, limes and citron: a chromosomal comparison. Cytogenet Genome Res 109: 276–282.PubMedCrossRefGoogle Scholar
  7. Cornélio MTMN, Figueirôa ARS, Santos KGB, Carvalho R, Soares Filho WS, Guerra M (2003) Chromosomal relationships among cultivars of Citrus reticulata Blanco, its hybrids and related species. Plant Syst Evol 240: 149–161.CrossRefGoogle Scholar
  8. Frost HB (1925) The chromosomes of Citrus. J Wash Acad Sci 15: 1–2.Google Scholar
  9. Gmitter FG, Jr (1993) ‘Marsh’ grapefruit. Fruit Var J 47: 130–133.Google Scholar
  10. Gmitter FG, Jr (1995) Origin, evolution, and breeding of the grapefruit. Plant Breed Rev 13: 345–363.Google Scholar
  11. Gottlob-McHugh SG, Lévesque M, MacKenzie K, Olson M, Yarosh O, Johnson DA (1990) Organization of the 5S rRNA genes in the soybean Glycine max (L.) Merrill and conservation of the 5S rDNA repeat structure in higher plants. Genome 33: 486–494.PubMedGoogle Scholar
  12. Guerra M (1993) Cytogenetics of Rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining. Heredity 71: 234–241.Google Scholar
  13. Guerra M, Pedrosa A, Silva AEB, Cornélio MTM, Santos KGB, Soares Filho WS (1997) Chromosome number and secondary constriction variation in 51 accessions of a Citrus germplasm bank. Braz J Genet 20: 489–496.CrossRefGoogle Scholar
  14. Guerra M, Santos KGB, Silva AEB, Ehrendorfer F (2000) Heterochromatin banding patterns in Rutaceae-Aurantioideae – a case of parallel chromosomal evolution. Am J Bot 87: 735–747.PubMedCrossRefGoogle Scholar
  15. Hodgson RW (1967) Horticultural varieties of Citrus. In Reuter W, Webber HJ, Batchelor LD, eds., The Citrus Industry. History, World Distribution, Botany and Varieties, vol. I. University of California Press, pp. 431–591.Google Scholar
  16. Kumamoto J, Scora RW, Lawton HW, Clerx WA (1986) Mystery of the forbidden fruit: historical epilogue on the origin of the grapefruit, Citrus paradisi (Rutaceae). Econ Bot 41: 97–107.Google Scholar
  17. Matsuyama T, Akihama T, Ito Y, Omura M, Fukui K (1996) Characterization of heterochromatic regions in ‘Trovita’ orange (Citrus sinensis Osbeck) chromosomes by the fluorescent staining and FISH methods. Genome 39: 941–945.PubMedGoogle Scholar
  18. Miranda M, Ikeda F, Endo T, Moriguchi T, Omura M (1997) Comparative analysis on the distribution of heterochromatin in Citrus, Poncirus and Fortunella chromosomes. Chromosome Res 5: 86–92.PubMedCrossRefGoogle Scholar
  19. Moore GA (2001) Oranges and lemons: clues to the taxonomy of Citrus from molecular markers. Trends Genet 17: 536–540.PubMedCrossRefGoogle Scholar
  20. Moscone EA, Matzke MA, Matzke AJM (1996) The use of combined FISH/GISH in conjunction with DAPI counterstaining to identify chromosomes containing transgene inserts in amphidiploid tobacco. Chromosoma 105: 231–236.Google Scholar
  21. Pedrosa A, Schweizer D, Guerra M (2000) Cytological heterozygosity and the hybrid origin of sweet-orange [Citrus sinensis (L.) Osbeck]. Theor Appl Genet 100: 361–367.CrossRefGoogle Scholar
  22. Robinson TR (1933) The origin of the Marsh seedless grapefruit. J Heredity 24: 437–439.Google Scholar
  23. Scora RW, Kumamoto J, Soost RK, Nauer EM (1982) Contribution to the origin of the grapefruit Citrus paradisi (Rutaceae). Syst Bot 7: 170–177.CrossRefGoogle Scholar
  24. Webber HJ (1943) Cultivated varieties of citrus. In Webber HJ, ed., The Citrus Industry. History, World Distribution, Botany and Varieties, vol. I. University of California Press, pp. 475–668.Google Scholar
  25. Yamamoto M, Tominaga S (2003) High chromosomal variability of mandarins (Citrus spp.) revealed by CMA banding. Euphytica 129: 267–274.CrossRefGoogle Scholar
  26. Yang X, Kitajima A, Hasegawa K (2002) Chromosome pairing set and the presence of unreduced gametes explain the possible origin of polyploid progenies from the diploids ‘Tosa-Butan’ × ‘Suisho-Butan’ pummelo. J Jpn Soc Hort Sci 71: 538–543.CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Ana Paula de Moraes
    • 1
  • Walter dos Santos Soares Filho
    • 2
  • Marcelo Guerra
    • 1
  1. 1.Laboratório de Citogenética Vegetal, Departamento de BotânicaUniversidade Federal de PernambucoRecifeBrazil
  2. 2.Embrapa Mandioca e Fruticultura TropicalCruz das AlmasBrazil

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