Drosophila under the lens: imaging from chromosomes to whole embryos
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Microscopy has been a very powerful tool for Drosophila research since its inception, proving to be essential for the evaluation of mutant phenotypes, the understanding of cellular and tissue physiology, and the illumination of complex biological questions. In this article we review the breadth of this field, making note of some of the seminal papers. We expand on the use of microscopy to study questions related to gene locus and nuclear architecture, presenting new data using fluorescence in-situ hybridization techniques that demonstrate the flexibility of Drosophila chromosomes. Finally, we review the burgeoning use of fluorescence in-vivo imaging methods to yield quantitative information about cellular processes.
Key wordschromosome Drosophila fluorescence microscopy FRAP GFP imaginal disc in-situ hybridization lacO arrays
We thank Dr. G. Ficz for providing the images of Pc-GFP and Ph-GFP expression in Drosophila embryos and larval tissues as well as for the FRAP curves presented here. We thank both Dr. Ficz and Dr. J. Post for helpful discussions.
- Bridges C (1935) Salivary chromosome maps: with a key to the banding of the chromosomes of Drosophila melanogaster. 26: 60–64.Google Scholar
- Grimaud C, Nègre N, Cavalli G (2006) From genetics to epigenetics: the tale of Polycomb group and trithorax group genes. Chromosome Res 14: 00–00 [refers to CHRO 1069].Google Scholar
- Kosman D, Reinitz J, Sharp D (1999) Automated assay of gene expression at cellular resolution. In Altman R, Dunker K, Hunter L, Klein T, eds., Proceedings of the 1998 Pacific Symposium on Biocomputing, pp. 6–17.Google Scholar
- Lifton R, Goldberg M, Karp R, Hogness D (1978) The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications. CSH Symp Quant Biol 42: 1047–1051.Google Scholar
- Miyawaki A (2006) New fluorescent probes and new perspective in bioscience. SPIE 6089: 18.Google Scholar
- Painter T (1934) Salivary chromosomes and the attack on the gene. J Hered 25: 465–476.Google Scholar