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Chromosome Research

, Volume 14, Issue 4, pp 451–464 | Cite as

Drosophila under the lens: imaging from chromosomes to whole embryos

  • Cornelia Fritsch
  • Ginette Ploeger
  • Donna J. Arndt-JovinEmail author
Article

Abstract

Microscopy has been a very powerful tool for Drosophila research since its inception, proving to be essential for the evaluation of mutant phenotypes, the understanding of cellular and tissue physiology, and the illumination of complex biological questions. In this article we review the breadth of this field, making note of some of the seminal papers. We expand on the use of microscopy to study questions related to gene locus and nuclear architecture, presenting new data using fluorescence in-situ hybridization techniques that demonstrate the flexibility of Drosophila chromosomes. Finally, we review the burgeoning use of fluorescence in-vivo imaging methods to yield quantitative information about cellular processes.

Key words

chromosome Drosophila fluorescence microscopy FRAP GFP imaginal disc in-situ hybridization lacO arrays 

Notes

Acknowledgements

We thank Dr. G. Ficz for providing the images of Pc-GFP and Ph-GFP expression in Drosophila embryos and larval tissues as well as for the FRAP curves presented here. We thank both Dr. Ficz and Dr. J. Post for helpful discussions.

Supplementary material

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Copyright information

© Springer 2006

Authors and Affiliations

  • Cornelia Fritsch
    • 1
  • Ginette Ploeger
    • 1
    • 2
  • Donna J. Arndt-Jovin
    • 1
    Email author
  1. 1.Department of Molecular BiologyMax Planck Institute for Biophysical ChemistryGöttingenGermany
  2. 2.Molecular Cytology, Swammerdam Institute for Life SciencesUniversity of AmsterdamAmsterdamThe Netherlands

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