Gene regulation and large-scale chromatin organization in the nucleus
Regulation of gene expression involves a number of different levels of organization in the cell nucleus. The main agents of transcriptional control are the cis-acting sequences in the immediate vicinity of a gene, which combine to form the functional unit or domain. Contacts between these sequences through the formation of chromatin loops forms the most basic level of organization. The activity of functional domains is also influenced by higher order chromatin structures that impede or permit access of factors to the genes. Epigenetic modifications can maintain and propagate these active or repressive chromatin structures across large genomic regions or even entire chromosomes. There is also evidence that transcription is organized into structures called ‘factories’ and that this can lead to inter-chromosomal contacts between genes that have the potential to influence their regulation.
Key wordschromatin chromosome functional domain gene regulation transcription
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- Burgess-Beusse B, Farrell C, Gaszner M et al. (2002) The insulation of genes from external enhancers and silencing chromatin. Proc Natl Acad Sci USA 99: 16433–16437.Google Scholar
- Carpenter A, Memedula S, Plutz M, Belmont A (2004) Common effects of acidic activators on large-scale chromatin structure and transcription. Mol Cell Biol 25: 958–968.Google Scholar
- Krajewski W, Becker PB (1998) Reconstitution of hyperacetylated, DNase I-sensitive chromatin characterized by high conformational flexibility of nucleosomal DNA. EMBO Rep 95: 1540–1545.Google Scholar
- Vogel J, von Heydebreck A, Purmann A, Sperling S (2005) Chromosomal clustering of a human transcriptome reveals regulatory background. BMC Bioinformatics 19: 6:230.Google Scholar