Base excision repair in nucleosome substrates
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Eukaryotic cells must repair DNA lesions within the context of chromatin. Much of our current understanding regarding the activity of enzymes involved in DNA repair processes comes from in-vitro studies utilizing naked DNA as a substrate. Here we review current literature investigating how enzymes involved in base excision repair (BER) contend with nucleosome substrates, and discuss the possibility that some of the activities involved in BER are compatible with the organization of DNA within nucleosomes. In addition, we examine evidence for the role of accessory factors, such as histone modification enzymes, and the role of the histone tail domains in moderating the activities of BER factors on nucleosomal substrates.
Key wordsbase excision repair chromatin nucleosome
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- Chafin DR, Hayes JJ (2001) Site-directed cleavage of DNA by linker histone-Fe(II) EDTA conjugates. Meth Mol Biol 148: 275–290.Google Scholar
- Friedberg EC (2003) DNA damage and repair. Nature 421: 436–440.Google Scholar
- Lee KM, Hayes JJ (1998) Linker DNA and H1-dependent reorganization of histone-DNA interactions within the nucleosome. Biochemistry 37: 8622–8628.Google Scholar
- Li G, Levitus M, Bustamante C, Widom J (2005) Rapid spontaneous accessibility of nucleosomal DNA. Nature Struct Mol Biol 12: 46–53.Google Scholar
- Seeberg E, Eide L, Bjoras M (1995) The base excision repair pathway. Trends Biol Sci 20: 391–397.Google Scholar
- Shrader TE, Crothers DM (1989) Artificial nucleosome positioning sequences. Proc Natl Acad Sci USA 86: 7418–7422.Google Scholar