TLR4/MyD88/NF-κB-Mediated Inflammation Contributes to Cardiac Dysfunction in Rats of PTSD
Post-traumatic stress disorder (PTSD) is related with myocardial injury and cardiac dysfunction, while the molecular mechanism has not been clear. This study investigated whether TLR4/MyD88/NF-κB-mediated inflammation involved in myocardial injury of PTSD. Adult male Wistar rats were exposed to single-prolonged stress (SPS), which was used broadly as a animal model of PTSD. Morris Water Maze (MWM) test and forced swimming test (FST) was carried out for behavioral testing. The protein expression of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) in the left ventricular of heart and TLR4/MyD88/NF-κB-mediated inflammation were examined. Our results showed that there were obvious increased in the protein expression of ANP and BNP in heart after exposure to SPS, SPS also significantly enhanced the serum level of IL-1β and TNF-α, and meanwhile, the TLR4/MyD88/NF-κB pathway were activated. These results demonstrated that the TLR4/MyD88/NF-κB pathway were involved in the myocardial injury of PTSD, which might be one of possible molecular mechanism contributed to the pathogenesis of cardiac dysfunction in PTSD.
KeywordsSingle-prolonged stress Post-traumatic stress disorder TLR4 MyD88 Nf-κb
This work was supported by Doctoral Scientific Research Fund from Liao Ning Province (No. 20170520016); Doctoral Scientific Research Fund from Shenyang Medical College (No. 20163047; No. 20171005 and No. 20181005). The authors thank the Laboratory of Cardiology in the First Hospital of China Medical University for valuable help in this experiment.
All authors mentioned in the paper have significantly contributed to the research. ML and YS conceived and designed the experiments, ML and JX performed the experiment and wrote the article, YS refined the article. All authors read and approved the final manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare no potential conflicts of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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